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Showing: 2061 - 2080 of 6494 results

  1. pAAV2-CAG-mScarlet-I-Cdt1

    Plasmid
    #191100
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    mScarlet-I-Cdt1 (30-120)
    Use
    AAV and Synthetic Biology
    Tags
    mScarlet-I fused to residues 30-120 of human Cdt1…
    Expression
    Mammalian
    Mutation
    Optimized to human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191100-plasmid-map-sequence-id-380122

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  3. pAAV-EFS-DIO-GRAB_CRF1.0

    Plasmid
    #208662
    Purpose
    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    EFS
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    208662-plasmid-map-sequence-id-417680

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  6. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S

    Plasmid
    #106183
    Purpose
    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Has Service
    AAV1
    Insert
    SF-Venus-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S
    Promoter
    hSynapsin
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    106183-plasmid-map-sequence-id-214796

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  9. AAV Ef1a-mRuby3-WPRE

    Plasmid
    #135427
    Purpose
    Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.
    Depositor
    Rylan Larsen
    Insert
    mRuby3
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    Feb. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    135427-plasmid-map-sequence-id-271155

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  10. pAAV asyn S87A/S129A

    Plasmid
    #36070
    Depositor
    Hilal Lashuel, Patrick Aebischer
    Insert
    alpha-synuclein S87A/S129A (SNCA Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S87A/S129A
    Promoter
    CMV
    Available since
    Sept. 10, 2012
    Availability
    Academic Institutions and Nonprofits only

  13. pAAV.hSyn.FLEX.FAPdL5-POST-T2A-dTomato.FLEX.WPRE.SV40

    Plasmid
    #105982
    Purpose
    This AAV plasmid in the presence of Cre recombinase expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.
    Depositor
    Marcel Bruchez
    Article
    Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19
    Insert
    FLEX-FAPdL5-POST-T2A-dTomato.FLEX
    Use
    AAV and Cre/Lox
    Tags
    FAPdL5-POST, T2A-dTomato, and myc
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin 1
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    105982-plasmid-map-sequence-id-235274

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  14. pAAV-EF1α1.1-Chronos-tdTomato

    Plasmid
    #122099
    Purpose
    AAV-mediated expression of Chronos-tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    Chronos-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α (1.1 kb short version)
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122099-plasmid-map-sequence-id-242263

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  16. pAAV-SEO-CaMKII-EGFP

    Plasmid
    #177018
    Purpose
    For expression of EGFP in a single-floxed, excisable open reading frame (cre-off), under control of the CaMKIIa promoter
    Depositor
    Peter Jonas
    Insert
    EGFP
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa
    Available since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177018-plasmid-map-sequence-id-353742

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  17. pAav-TP53-T2A-BirA*

    Plasmid
    #115652
    Purpose
    rAAV-based donor template for genome engineering of the TP53 protein C-terminus containing a T2A-BirA* module and a selection cassette
    Depositor
    Sven Eyckerman
    Article
    Vandemoortele et al J Proteome Res. 2018 Dec 10. doi: 10.102
    Inserts
    TP53 (TP53 Human)
    TP53 (TP53 Human)
    T2A
    BirA*
    Use
    AAV
    Tags
    BirA* and T2A
    Expression
    Mutation
    Homology region 1 and Homology region 2
    Promoter
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    115652-plasmid-map-sequence-id-223051

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Showing: 2061 - 2080 of 6494 results