8,734 results

1. NYESO beta/alpha into TRAC HDRT Source (pTR 169)

   Plasmid

   #112021

   Purpose

   DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   NYESO beta/alpha into TRAC HDRT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-C1-TBP

   Plasmid

   #26674

   Depositor

   Sui Huang

   Article

   Chen et al Mol Biol Cell. 2002 Jan . 13(1):276-84.

   Insert

   TBP (TBP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-PfV-Sapphire-IRES-DSRed

   Plasmid

   #116934

   Purpose

   Mammalian lenti vector for expression of the 40nm GEM and DSRed

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   Pfv
   

   Use

   Lentiviral

   Tags

   Sapphire

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB_tre_dCas9_VP160

   Plasmid

   #126031

   Purpose

   PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Inserts

   dCas9 fusion with VP160
   Hygromycin resistance
   

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   EF1 alpha and TRE

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSFV3

   Plasmid

   #92072

   Purpose

   Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.

   Depositor

   Henrik Garoff

   Article

   Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

   Type

   Empty backbone

   Use

   Empty expression plasmid, for run-off transcripti…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-HNRNPA1C-mCh-Cry2WT

   Plasmid

   #101226

   Purpose

   hnRNPA1(186-320) fused to mCh-Cry2WT

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

   Insert

   hnRNPA1(186-320) (HNRNPA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)

   Plasmid

   #31476

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   SFFV d14 GFP donor EF1s I-SceI
   

   Use

   Lentiviral

   Tags

   HA and NLS

   Expression

   Mutation

   GFP has 14 amino acids deleted from c terminus

   Promoter

   Available since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXL010-Wnt dual (GFP-Fire) reporter

   Plasmid

   #40588

   Depositor

   Sean Palecek

   Article

   Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

   Insert

   Wnt reporter sequences
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Wnt promoter

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCerulean3-N1

   Plasmid

   #54730

   Purpose

   Localization: N1 Cloning Vector, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Type

   Empty backbone

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. mito-dendra2

    Plasmid

    #55796

    Purpose

    photo-activatable mitochondrial marker for expression in mammalian cells

    Depositor

    David Chan

    Article

    Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10

    Insert

    mito-Dendra2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-CjCas9

    Plasmid

    #89754

    Purpose

    Expression of CjCas9 with His tag in E.coli

    Depositor

    Seokjoong Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    6XHis, HA, and SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT3-EF1a-c-Met

    Plasmid

    #31784

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    Met (MET Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-U6-sgRNA-CMV-GFP

    Plasmid

    #85451

    Purpose

    Express sgRNA in mammalian cells

    Depositor

    Hetian Lei

    Article

    Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

    Insert

    pCMV-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pCMV-EGFP

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-FLEX-DTR-GFP

    Plasmid

    #124364

    Purpose

    conditional expression of a diphtheria toxin receptor (DTR)–GFP fusion protein

    Depositor

    Eiman Azim, Thomas Jessell

    Article

    Azim et al Nature. 2014 Apr 17;508(7496):357-63. do

    Has Service

    AAV2

    Insert

    DTR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Chicken B-actin

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV4-ApoE3

    Plasmid

    #87086

    Purpose

    Expresses ApoE3 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    Apolipoprotein E3 (APOE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGADT7-GW

    Plasmid

    #61702

    Purpose

    Gateway compatible Y2H vector - AD

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCV-Myc-IRES-RFP

    Plasmid

    #35395

    Depositor

    Martine Roussel

    Article

    Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

    Inserts

    Myc (Myc Mouse)
    RFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pNEU-hMbPylRS-4xU6M15

    Plasmid

    #105830

    Purpose

    Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells

    Depositor

    Irene Coin

    Article

    Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464

    Inserts

    Humanized MbPylRS
    tRNAM15
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    (Y271A, Y349F)MbPylRS

    Promoter

    CMV and U6

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-GFP

    Plasmid

    #32395

    Depositor

    John T Gray

    Article

    Gray et al Methods Mol Biol. 2011;807:25-46.

    Insert

    GFP
    

    Use

    AAV; Adeno-associated virus

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGWB401

    Plasmid

    #74795

    Purpose

    Gateway cloning compatible binary vector for simple cloning of gene.

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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