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p-cyclinB1-mcherry (1958)

Plasmid

#26063

Depositor

Jonathon Pines

Article

Gavet et al Dev Cell. 2010 Apr 20. 18(4):533-43.

Insert

Cyclin B1 (CCNB1 Human)

Tags

mcherry

Expression

Mammalian

Available Since

Sept. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pJSK659

Plasmid

#205128

Purpose

Expression of GFP-SnxA in mammalian cells

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

SnxA

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSAM_Ec

Plasmid

#102939

Purpose

Transposon mutagenesis and Tn-seq

Depositor

Matthew Mulvey

Article

Wiles et al PLoS Genet. 2013;9(8):e1003716. doi: 10.

Insert

mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase

Expression

Bacterial

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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sox10:mCherry

Plasmid

#98695

Purpose

I-SceI meganuclease-mediated mCherry transgenesis in zebrafish

Depositor

Thomas Look

Article

Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

Insert

mCherry

Use

Zebrafish expression

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLX302 Luciferase-V5 puro

Plasmid

#47553

Purpose

Lentiviral expression of V5-tagged Luciferase in mammalian cells

Depositor

Kevin Janes

Article

Kang et al Sci Signal. 2013 Aug 6;6(287):rs13. doi:

Insert

Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pG106 - KA

Plasmid

#178041

Purpose

Bacteroides - Escherichia coli shuttle vector with ErmF and ErmAM, Kan and Amp selection markers

Depositor

Janina Lewis

Article

Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

Type

Empty backbone

Use

Shuttle vector

Expression

Bacterial

Available Since

Feb. 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pT7-CalfluxVTN

Plasmid

#83926

Purpose

Bioluminescent Reporter of Intracellular Calcium

Depositor

Carl Johnson

Article

Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

Insert

CalfluxVTN

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

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BASIC_SEVA_67_pKD46.10

Plasmid

#178942

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available Since

March 1, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pJV0042

Plasmid

#205138

Purpose

Expression of 6xHis-EGFP-SnxA in bacteria

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

SnxA

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEM790

Plasmid

#242607

Purpose

T-DNA plasmid, Level 2 destination vector, replaces pMIN-VS1

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pBSDONR P1-P4

Plasmid

#71744

Purpose

ampicillin resistant derivative of pDONR221 P1-P4 for multisite GATEWAY cloning

Depositor

Roger Innes

Article

Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

Type

Empty backbone

Use

Gateway entry vector for use in multisite gateway…

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSHS212

Plasmid

#87346

Purpose

Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/C574S

Promoter

T7

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pSWITCH

Plasmid

#222402

Purpose

Expression in Streptococcus pyogenes

Depositor

Anne Botteaux

Article

Schiavolin et al Microbiol Spectr. 2024 Oct 3;12(10):e011

Type

Empty backbone

Expression

Bacterial

Available Since

July 2, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pMIN-Ri

Plasmid

#197784

Purpose

Level 2 destination vector: pMIN-Ri backbone

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-GFP1

Plasmid

#106408

Purpose

P-RAN-GFP1 (RANbody GFP1::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-GFP1

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA HA HP1 gamma

Plasmid

#24080

Depositor

Naoko Tanese

Article

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 gamma (CBX3 Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only

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BASIC_SEVA_27_pKD46.10

Plasmid

#178922

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available Since

Feb. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-postASAP

Plasmid

#178792

Purpose

Expresses voltage sensor targeted to dendrites and spines

Depositor

Rafael Yuste

Article

Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

Insert

FingR.PSD95 and ASAP2f

Expression

Mammalian

Mutation

mutations in amino acids L146G, S147T N149R, S150…

Promoter

CAG

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCAH01Sp::Cas9

Plasmid

#128160

Purpose

Ptet-controlled expression of S. pyogenes Cas9

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Insert

Sp Cas9

Expression

Bacterial

Promoter

Ptet

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pUF-Cas9-pre-sgRNA

Plasmid

#137845

Purpose

Cas9 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Type

Empty backbone

Use

CRISPR

Available Since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJPC062

Plasmid

#234534

Purpose

TRV2 expressing CinH

Depositor

Daniel Voytas

Article

Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

Insert

CinH Recombinase

Use

Recombinase

Expression

Plant

Promoter

CaMV 35S

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pEC2483 (pSpy1C)

Plasmid

#220248

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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DS-SPcas

Plasmid

#48645

Purpose

Bacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycin

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
tracrRNA precursor

Use

CRISPR

Promoter

proC and tracrRNA promoter

Available Since

Dec. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-H2A2B

Plasmid

#106412

Purpose

P-RAN-H2A2B (RANbody H2A2B::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-H2A2B

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCBL102-RUBY

Plasmid

#210379

Purpose

Expresses betalain biosynthesis genes under CaMV 35S promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

35S-RUBY

Expression

Plant

Available Since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMC-1-ccdB-6

Plasmid

#197811

Purpose

Level 0 J1-J6 destination vector, BbsI entry & CcdB selection

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-ccdB-9

Plasmid

#197812

Purpose

Level 0 J6-J9 destination vector, BbsI entry & CcdB selection

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-9-ccdB-11

Plasmid

#197813

Purpose

Level 0 J9-J11 destination vector, BbsI entry & CcdB selection

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pccGFPG83I

Plasmid

#73650

Purpose

Fluorescent reporter for transmembrane protein dimerization negative control.

Depositor

Alessandro Senes

Article

Armstrong et al Biochim Biophys Acta. 2016 Jul 21. pii:

Insert

glycophorin A- G83I

Expression

Bacterial

Mutation

glycine 83 to isoleucine

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-Venus-Flag (865)

Plasmid

#40998

Depositor

Jonathon Pines

Article

Sedgwick et al EMBO J. 2013 Jan 23;32(2):303-14. doi: 1

Type

Empty backbone

Tags

3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only