We narrowed to 76,979 results for: Rest

1. pUC-ChREBPα

   Plasmid

   #211754

   Purpose

   Cloning vector for the expression of mouse ChREBPα

   Depositor

   Russell Goodman

   Article

   Singh et al Cell Metab. 2024 Jan 2;36(1):144-158.e7.

   Insert

   MLXIPL (Mlxipl Mouse)
   

   Use

   Cloning vector

   Available Since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223_HyPer7RgDAAO

   Plasmid

   #217746

   Purpose

   Donor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane.

   Depositor

   Seth Parker

   Article

   Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

   Insert

   HyPer7 D amino acid oxidase
   

   Tags

   Nuclear export signal

   Expression

   Mammalian

   Mutation

   Fused DAAO to the C-terminus of HyPer7 using a Gl…

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pINDUCER21-ERG

   Plasmid

   #51301

   Purpose

   Expression of ERG after doxycycline administration

   Depositor

   George Daley

   Article

   Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

   Insert

   v-ets avian erythroblastosis virus E26 oncogene homolog (ERG Human)
   

   Use

   Lentiviral

   Tags

   n/a

   Mutation

   full length ORF

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSW002-Pc-TorA(sp)-mNeonGreen

   Plasmid

   #205020

   Purpose

   Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   TorA-mNeonGreen
   

   Expression

   Bacterial

   Mutation

   mNeonGreen is codon optimized for expression in P…

   Promoter

   Pc

   Available Since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag-ZDHHC9-C169S

   Plasmid

   #231673

   Purpose

   Expresses human ZDHHC9-C169S protein

   Depositor

   Chunhong Ma

   Article

   Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

   Insert

   ZDHHC9 (ZDHHC9 Human)
   

   Tags

   3×Flag Tag

   Expression

   Mammalian

   Available Since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)

   Plasmid

   #114103

   Purpose

   fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO3) for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Petersen et al Front Neurol. 2022 Jan 20;12:792643. doi

   Insert

   sbGLuc-VChR1-EYFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   motoneuron-specific Homeobox 9 promoter

   Available Since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-TRE-DIO-Casp3-TEV

   Plasmid

   #183766

   Purpose

   This plasmid is for use with neuronal cell-type selective activity tagging. The Caspase3 expression requires both neural activity and Cre recombinase.

   Depositor

   William Wisden

   Article

   Yu et al Science. 2022 Jul;377(6601):63-72. doi:

   Insert

   AAV transgene - tetO promoter-flex/DIO-Caspase3-TEV
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Expression

   Mammalian

   Promoter

   tetO promoter

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. GSK3A_pLX307

   Plasmid

   #98341

   Purpose

   Lentiviral expression of GSK3A

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   GSK3A (GSK3A Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-BsmbI-LoxP-Ef1a-EM7-mCherry-Neo-PolyA-LoxP

   Plasmid

   #192822

   Purpose

   Golden Gate compatible plasmid with mCherry and Neo

   Depositor

   Mark Denham

   Article

   Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

   Insert

   mCherry and Neo
   

   Expression

   Mammalian

   Available Since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-TRE-FH-TET3 short

    Plasmid

    #166916

    Purpose

    Lentivirus for inducible overexpression of human TET3 short isoform lacking CXXC domain (NM_001366022.1)

    Depositor

    Roland Friedel

    Article

    Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

    Insert

    TET3 short isoform
    

    Use

    Lentiviral

    Tags

    HA Tag

    Expression

    Mammalian

    Mutation

    Changed nucleotide 18 (G) to (A) to delete FseI r…

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFRLuc-CoV2-FLucStop

    Plasmid

    #177617

    Purpose

    Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. In-frame stop codon inserted after FLuc (negative control).

    Depositor

    Junjie Guo

    Article

    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

    Insert

    SARS-CoV-2 FSE
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    In-frame stop codon added between FLuc and FSE

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRIP-CMV-puro-2A-BLM

    Plasmid

    #127641

    Purpose

    Over-expression of human BLM

    Depositor

    Nicolas Manel

    Article

    Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

    Insert

    BLM (BLM Human)
    

    Use

    Lentiviral

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

    Plasmid

    #68717

    Purpose

    Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

    Depositor

    Mark Huebener, Tobias Rose, Tobias Bonhoeffer

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Has Service

    AAV1

    Insert

    mRuby2-P2A-GCaMP6s
    

    Use

    AAV

    Mutation

    Cre-dependent expression from inverted open readi…

    Promoter

    CAG-FLEX

    Available Since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-HP1α-miRFP670-Cry2WT

    Plasmid

    #122442

    Purpose

    Expresses disordered protein HP1α fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    HP1 alpha (CBX5 Human)
    

    Use

    Lentiviral

    Tags

    Cry2WT and miRFP670

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. UQCRB_pLX307

    Plasmid

    #98381

    Purpose

    Lentiviral expression of UQCRB

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    UQCRB (UQCRB Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

    Plasmid

    #68720

    Purpose

    Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

    Depositor

    Mark Huebener, Tobias Rose, Tobias Bonhoeffer

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Has Service

    AAV1

    Insert

    mRuby2-P2A-GCaMP6s
    

    Use

    AAV

    Mutation

    Cre-dependent expression from inverted open readi…

    Promoter

    hSyn1-FLEX

    Available Since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. EMR2_pLX307

    Plasmid

    #98332

    Purpose

    Lentiviral expression of EMR2

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    EMR2 (ADGRE2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pODN-H2BC11-mR2

    Plasmid

    #183866

    Purpose

    Repair template for the C-terminal tagging of H2B histones with mRuby2 in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    H2BC11 homology arms with linker-mRuby2 (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mRuby2

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pODN-mR2-MYH10

    Plasmid

    #183869

    Purpose

    Repair template for the N-terminal tagging of myosin heavy chain 10 with mRuby2 in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    MYH10 homology arms with mRuby2-linker (MYH10 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mRuby2-linker

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBabe_puro_DEST_Flag_SOX2

    Plasmid

    #45270

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    MCOPS3 (SOX2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

    Plasmid

    #87827

    Purpose

    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Promoter

    CMV promoter, tetracycline operator

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. LoxP2-Mb2Tomato-2A (SO240)

    Plasmid

    #99614

    Purpose

    To clone gene of interest downstream of LoxP2-Mb2Tomato-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Mb2Tomato
    

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pSW002-Pc-TorT(sp)-mNeonGreen

    Plasmid

    #205021

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    TorT-mNeonGreen
    

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    Pc

    Available Since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pBabe_puro_DEST_Flag_FNDC3B

    Plasmid

    #45269

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    FNDC3B (FNDC3B Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pcDNA5 FRT TO GFP MPS1 K-R

    Plasmid

    #59819

    Purpose

    Allows the integration of GFP MPS1 K-R in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Tighe et al J Cell Biol. 2008 Jun 16;181(6):893-901.

    Insert

    Mps1 (TTK Human)
    

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    KD, catalytically inactive, D664A

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}

    Plasmid

    #184381

    Purpose

    This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

    Depositor

    Ori Bar-Nur

    Article

    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

    Insert

    Cas9, two gRNAs targeting dystrophin
    

    Use

    Lentiviral

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pBabe_puro_DEST_Flag_GPR160

    Plasmid

    #45274

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    GPR160 (GPR160 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

28. lenti-Cas9-VQR-GFP_activity_reporter

    Plasmid

    #87156

    Purpose

    This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).

    Depositor

    Daniel Bauer

    Article

    Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

    Insert

    GFP and sgRNA targeting GFP (NGA restricted)
    

    Use

    Lentiviral

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. RCAS-sgATG7

    Plasmid

    #75228

    Purpose

    An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh

    Depositor

    Noor Gammoh

    Article

    Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:

    Insert

    Autophagy Related 7 (Atg7 Mouse)
    

    Use

    Retroviral

    Promoter

    U6

    Available Since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pWzl_neo_DEST_flag_SOX2

    Plasmid

    #45309

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    MCOPS3 (SOX2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only