We narrowed to 33,255 results for: IND

1. pGEXNKI-GST-3C-LIC

   Plasmid

   #108710

   Purpose

   Expression of a GST-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Use

   Tags

   GST-3C protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pet23a_RBPMS_FL

   Plasmid

   #59392

   Purpose

   Plasmid for bacterial overexpression of RBPMS

   Depositor

   Thomas Tuschl

   Article

   Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSW554 - pOp5:SP-mVenus-HDEL

   Plasmid

   #116006

   Purpose

   destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

   Depositor

   Sebastien Wolf

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pOp5:SP(ER)-mVenus-HDEL:tUBQ10::SulfR
   

   Use

   Tags

   HDEL and SP(ER)

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-4T1-Claspin-MΔN

   Plasmid

   #31624

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

   Insert

   Claspin (CLSPN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 899-1206 only

   Promoter

   Available Since

   Nov. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pETNKI-strepII-3C-LIC-kan

   Plasmid

   #108706

   Purpose

   Expression of a StrepII-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Use

   Tags

   StrepII-3C protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA4/TO CD16.7-h-PKD1 FLM R4114A

   Plasmid

   #83461

   Purpose

   Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (503)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   Polycystin-1 (PKD1 Human)
   

   Use

   Tags

   CD16.7

   Expression

   Mammalian

   Mutation

   Amino-acids 4078-4303, R4113A

   Promoter

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LB334 (Bcl-2 from -1287 to -1640)

   Plasmid

   #15241

   Depositor

   Linda Boxer

   Article

   Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

   Insert

   B-cell lymphoma/leukemia-2 (BCL2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Bacterial

   Mutation

   Bcl-2 fragment from -1287 to -1640 with P1

   Promoter

   Available Since

   Aug. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pCAG eGFP-Oct6

   Plasmid

   #203790

   Purpose

   Competitive assay of Oct4 and Oct6 binding to DNA motifs.

   Depositor

   Thorsten Wohland

   Article

   Mistri et al EMBO Rep. 2015 Sep;16(9):1177-91. doi: 1

   Insert

   Pou3f1 (Pou3f1 Mouse)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA1-GATA4-opposite orientation

   Plasmid

   #51928

   Purpose

   for T7 directed antisense production of mouse GATA4

   Depositor

   Jeffery Molkentin

   Article

   Liang et al J Biol Chem. 2001 Aug 10;276(32):30245-5

   Insert

   GATA4 reverse orientation (Gata4 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 28, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pQE60-mPU.1-ETS

    Plasmid

    #85733

    Purpose

    Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272, in M15 cells already pre-transformed with 2nd plasmid, pREP-4

    Depositor

    Gregory Poon

    Article

    Poon et al J Biol Chem. 2012 May 25;287(22):18297-3

    Insert

    ETS domain of transcription factor PU.1 (Spi1 Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV epArcLight

    Plasmid

    #85803

    Purpose

    genetically-encoded fluorescent voltage indicator

    Depositor

    Vincent Pieribone

    Article

    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

    Insert

    epArcLight
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; ecliptic pHluorin…

    Promoter

    CMV

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. mNeongreen-pGBD(PKN1 codon optimized)

    Plasmid

    #129634

    Purpose

    Visualization of active RhoA

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

    Insert

    RhoA binding doamin of PKN1(30 - 100) codon optimized
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVdel

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. HOXA9 (murine) FLAG pRC/CMV

    Plasmid

    #8514

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXA9 (Hoxa9 Mouse)
    

    Use

    Tags

    FLAG and His

    Expression

    Mammalian

    Mutation

    FLAG epitope tag replaces T7 tag in the pET back…

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pJM240 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-aacC1)

    Plasmid

    #110561

    Purpose

    miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insert

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    stRBS-aacC1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhaSR-PrhaBAD

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. PHR-GFP

    Plasmid

    #183926

    Purpose

    Optogenetic PHR domain coupled to GFP; binds to CIBN upon blue light exposure

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    PHR-GFP (CRY2 Mustard Weed, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET23a_mod_RBPMS_FL

    Plasmid

    #59393

    Purpose

    Plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    6xHis, FLAG, and HA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hisSUMO-Gli1

    Plasmid

    #87225

    Purpose

    Express hisSUMO tagged DNA binding domain of Gli1(mouse) in E. coli

    Depositor

    Gary Stormo

    Article

    Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

    Insert

    GLI1 (Gli1 Mouse)
    

    Use

    Tags

    hisSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. LB360 (Bcl-2 from -1287 to -1337)

    Plasmid

    #15242

    Depositor

    Linda Boxer

    Article

    Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

    Insert

    B-cell lymphoma/leukemia-2 (BCL2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Bacterial

    Mutation

    Bcl-2 fragment from -1287 to -1337 with P1

    Promoter

    Available Since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA-p160(1-200)

    Plasmid

    #43

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 myb binding protein (Mybbp1a Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pET28a His6Flag2AUF1(p42)

    Plasmid

    #135941

    Purpose

    Expresses AUF1p42 in E. coli

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
    

    Use

    Tags

    Flag2 and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pVL94 - pOp4:VND7

    Plasmid

    #116016

    Purpose

    destination vector for GreenGate cloning method, "Effector line", crossed with a "Driver line" the GOI, here VND7, will be expressed upon Dex-induction

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPN461

    Plasmid

    #137877

    Purpose

    PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRi; mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    POGZ (POGZ Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRS306Sup35R1

    Plasmid

    #17715

    Depositor

    Susan Lindquist

    Article

    Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

    Insert

    pRS306Sup35R1 (SUP35 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    An pRS306 based integrating plasmid designed for …

    Promoter

    Available Since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3myc-PR-LBD

    Plasmid

    #89113

    Purpose

    mammalian expression of human progesterone receptor ligand binding domain amino acid residues 632-933 with N-terminal myc tag

    Depositor

    Elizabeth Wilson

    Article

    Liu et al J Biol Chem. 2011 Aug 26;286(34):29951-6

    Insert

    human progesterone receptor ligand binding domain (PGR Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    contains Ligand Binding Domain (632-933)

    Promoter

    CMV

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

25. pJS278

    Plasmid

    #134139

    Purpose

    Bacterial expression of human Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-Precission-human Ska1 MTBD (SKA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    aa 132-255, R155A

    Promoter

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRP_8xgRNA-FLuc

    Plasmid

    #135937

    Purpose

    Firefly luciferase reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minCMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pETDUET1-hUCH37 (ISF2)-NFRKB(39-156)

    Plasmid

    #61938

    Purpose

    Co-expression of hUCH37 (ISF2) and hNFRKB (39-156) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF2) (UCHL5 Human)
    hNFRKB (39-156) (NFRKB Human)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 156, residues 2-38 dele…

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pET151-GFP-PP-RPN2 (925-953)

    Plasmid

    #73744

    Purpose

    Expression of GFP-PP-RPN2 (925-953) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Use

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 932-953 of RPN2

    Promoter

    T7

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

29. pET28a His6Flag2AUF1(p40)

    Plasmid

    #135940

    Purpose

    Expresses AUF1p40 in E. coli

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
    

    Use

    Tags

    Flag2 and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPN460

    Plasmid

    #137878

    Purpose

    PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRa;mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    POGZ (POGZ Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGEX-4T1-Claspin-BPII

    Plasmid

    #31632

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

    Insert

    Claspin (CLSPN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 492-622 of claspin

    Promoter

    Available Since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

32. pTet02

    Plasmid

    #78284

    Purpose

    mRFP1 expressed behind an ATc-inducible construct

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    Tet inducible mRFP1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pWJPrP1

    Plasmid

    #22109

    Depositor

    Susan Lindquist

    Article

    Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

    Insert

    mouse PrP ORF insert (Prnp Mouse)
    

    Use

    Construction of targeting vector

    Tags

    Expression

    Mutation

    mutations at aa 108 and 111 to make 3F4 epitope (…

    Promoter

    Available Since

    Oct. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

34. sg resistant gamma-tubulin

    Plasmid

    #104433

    Purpose

    For expressing gamma-tubulin in cells expressing γTUBULIN single guide RNA

    Depositor

    Maria Alvarado-Kristensson

    Article

    Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    changed t bp28 to c; g bp30 to a; c bp33 to a; …

    Promoter

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pENTR4-RBPMS

    Plasmid

    #59387

    Purpose

    Entry vector with RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET151-GFP-PP-RPN2 (918-932)

    Plasmid

    #73748

    Purpose

    Expression of GFP-PP-RPN2 (918-932) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Use

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 918-932 of RPN2

    Promoter

    T7

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

37. pTXB1-xlH2B(1-114)_K114A

    Plasmid

    #118217

    Purpose

    Bacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    xlH2B(1-114)
    

    Use

    Tags

    Mxe-GyrA intein fused to chitin binding domain

    Expression

    Bacterial

    Mutation

    Lysine 114 to alanine

    Promoter

    T7

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LentiV_Neo_SIK3_T142Q

    Plasmid

    #108110

    Purpose

    Lentiviral expression plasmid of human SIK3 cDNA (gatekeeper mutation) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    SIK3 (SIK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    change Threonine 142 to Glutamine

    Promoter

    EFS promoter

    Available Since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pETNKI-his-3C-LIC-Amp

    Plasmid

    #108704

    Purpose

    Expression of a His-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    His-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. FgH1tUTG_huMcl-1.2

    Plasmid

    #85531

    Purpose

    Inducible expression of guide RNA (huMcl-1.2) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    hu Mcl-1.2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1t

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge