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We narrowed to 5,553 results for: pcDNA3.1+

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Showing: 5151 - 5200 of 5553 results

  5. pTE3334

    Plasmid
    #107537
    Purpose
    Expresses Mb crRNA and human codon optimized MbCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Mb crRNA
    hMbCpf1(RVR mutant)
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N576R, K582V, N586R
    Promoter
    CMV and human U6
    Available Since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107537-plasmid-map-sequence-id-207346

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  6. mCerulean-proα2(I)

    Plasmid
    #119836
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mCerulean between signal sequence and exon 6
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-5 replaced by fluorescent tag
    Promoter
    CMV
    Available Since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119836-plasmid-map-sequence-id-233866

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  8. pTE3330

    Plasmid
    #107534
    Purpose
    Expresses Lb crRNA and human codon optimized LbCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Lb crRNA
    hLbCpf1(RVR mutant)
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    G532R, K538V, Y542R
    Promoter
    CMV and human U6
    Available Since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107534-plasmid-map-sequence-id-207314

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  10. pcDNA3-sACE2v2.2-8his

    Plasmid
    #149663
    Purpose
    Mammalian expression plasmid for his-tagged sACE2 high affinity variant 2.2
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Short GSG linker and 8his tag
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    149663-plasmid-map-sequence-id-298413

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  14. pTE3336

    Plasmid
    #107541
    Purpose
    Expresses Mb crRNA and human codon optimized MbCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Mb crRNA
    hMbCpf1(RR mutant)
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N576R, K637R
    Promoter
    CMV and human U6
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107541-plasmid-map-sequence-id-207245

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  15. pTE3333

    Plasmid
    #107540
    Purpose
    Expresses Fn crRNA and human codon optimized FnCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Fn crRNA
    hFnCpf1(RR mutant)
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N607R, K671R
    Promoter
    CMV and human U6
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107540-plasmid-map-sequence-id-207239

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  16. pTE3331

    Plasmid
    #107536
    Purpose
    Expresses Fn crRNA and human codon optimized FnCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Fn crRNA
    hFnCpf1(RVR mutant)
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N607R, K613V, N617R
    Promoter
    CMV and human U6
    Available Since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107536-plasmid-map-sequence-id-207338

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  21. PKA-C-HiBiT

    Plasmid
    #211378
    Purpose
    Mammalian expressed PKA-C-HiBiT with 10xHis tag
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Human protein kinase cAMP-activated catalytic subunit alpha (PRKACA) transcript variant 1, with C-terminal HiBiT (PRKACA Human)
    Tags
    10x His-tag with thrombin site and HiBiT Tag
    Expression
    Mammalian
    Mutation
    Kozak sequence for mammalian expression
    Promoter
    T7 Promotor
    Available Since
    June 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    211378-plasmid-map-sequence-id-417162

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  23. AcrVA1_cpGR2(GGS5)-S103ΔELS_(GGS)2-(GGS)2

    Plasmid
    #246051
    Purpose
    Expression of AcrVA1-cpGR2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS and GGS2 linkers; enables chemogenetic control of MbCas12a
    Depositor
    Dominik Niopek
    Article
    Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk
    Insert
    AcrVA1_cpGR2-S103ΔELS
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Mutation
    ΔELS
    Available Since
    Dec. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    246051-plasmid-map-sequence-id-495343

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  24. Neo-2/15-TGFa-SPMn-SpyTag003

    Plasmid
    #246650
    Purpose
    Expresses Neo-2/15-TGFa-SPMn-SpyTag003 in mammalian cells for calcium-induced NeissLock protein ligation to epidermal growth factor receptor (EGFR)
    Depositor
    Mark Howarth
    Article
    Lim et al ACS Chem Biol. 2025 Sep 15. doi: 10.1021
    Insert
    Neo-2/15-TGFa-SPMn-SpyTag003
    Tags
    Signal peptide and SpyTag003
    Expression
    Mammalian
    Mutation
    Construct contains the tPA signal peptide, Neo2/…
    Promoter
    CMV promoter
    Available Since
    Oct. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    246650-plasmid-map-sequence-id-491288

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  25. MPC1-AA-FLAG

    Plasmid
    #228904
    Purpose
    Expression of mouse MPC1 in which K45 and K46 motif sites were replaced with alanine blocking acetylation with a C terminal FLAG tag
    Depositor
    Ghazaleh Ashrafi
    Article
    Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do
    Insert
    MPC1 (Mpc1 Mouse)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    K45 and K46 motif sites were both replaced with a…
    Promoter
    CMV
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228904-plasmid-map-sequence-id-460265

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  26. TadA-8e-eIscBn-C-HMG-D

    Plasmid
    #227145
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain in C terminus driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-TadA-8e-linker-nOgeuIscB-v3-linker-HMG-D-bpNLS
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227145-plasmid-map-sequence-id-453986

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  27. eIscB-D

    Plasmid
    #227017
    Purpose
    Vector encoding human codon-optimized enhanced-activity OgeuIscB fusion with HMD-D domain and extra SV40 NLS driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    SV40 NLS-HMG-D-linker-OgeuIscB-v3-SV40-nucleoplasmin NLS
    Mutation
    D96R/E84R/V159R
    Promoter
    CMV
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227017-plasmid-map-sequence-id-453985

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  28. TadA-8e-eIscBn

    Plasmid
    #227018
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-TadA-8e-linker-nOgeuIscB-v3-bpNLS
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227018-plasmid-map-sequence-id-453983

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  29. hA3A*-IscBn

    Plasmid
    #227146
    Purpose
    Vector encoding human codon-optimized nOgeuIscB fusion with two copies of UGI and hA3A* variant driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-hA3A*-linker-nOgeuIscB-nucleoplasmin NLS-2XUGI-bpNLS
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227146-plasmid-map-sequence-id-453980

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  30. TadA-8e-eIscBn-N-HMG-D

    Plasmid
    #227143
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain in N terminus driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-HMG-D-linker-TadA-8e-linker-nOgeuIscB-v3-bpNLS
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227143-plasmid-map-sequence-id-453972

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  32. pCDNA3-2xMARS-PLD

    Plasmid
    #205237
    Purpose
    Expresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMF
    Depositor
    Jeremy Baskin
    Article
    Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816
    Inserts
    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    phospholipase D (PLD)
    Tags
    Nuclear Export Sequence and mCherry
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available Since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205237-plasmid-map-sequence-id-404742

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  38. Myc-Nix-S35A

    Plasmid
    #197562
    Purpose
    Mammalian expression of myc-tagged human Nix with an alanine substitution of serine-35. Based on Myc-Nix (#100795)
    Depositor
    Joseph Gordon
    Article
    Field et al Autophagy. 2025 Jul;21(7):1544-1555. doi
    Insert
    BCL2 interacting protein 3 like (BNIP3L Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Serine 35 changed to alanine
    Promoter
    CMV
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197562-plasmid-map-sequence-id-390527

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  44. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG E1322R

    Plasmid
    #166814
    Purpose
    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity
    Depositor
    Hao Wu
    Article
    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586
    Insert
    NLRP1 (NLRP1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E1322R
    Promoter
    CMV
    Available Since
    May 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    166814-plasmid-map-sequence-id-331962

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  45. pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R K1277E

    Plasmid
    #166811
    Purpose
    Mammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association, K1277E abolishes UPA-UPA contacts
    Depositor
    Hao Wu
    Article
    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586
    Insert
    NLRP1 (NLRP1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    LL1193EE, P1214R, K1277E
    Promoter
    CMV
    Available Since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166811-plasmid-map-sequence-id-328312

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  50. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG H1276G

    Plasmid
    #166812
    Purpose
    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity
    Depositor
    Hao Wu
    Article
    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586
    Insert
    NLRP1 (NLRP1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    H1276G
    Promoter
    CMV
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166812-plasmid-map-sequence-id-328391

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Showing: 5151 - 5200 of 5553 results