We narrowed to 9,811 results for: Met;

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pUCBB-ntH6-eGFP

Plasmid

#32557

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

6 His tag with Thrombin site

Expression

Bacterial

Promoter

Consitutive lac

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pSKA397

Plasmid

#80380

Purpose

mini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.

Depositor

Mustafa Khammash

Article

Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

Inserts

cat
metE

Use

Synthetic Biology; Mini-tn7 delivery vector

Expression

Bacterial

Promoter

cat promoter and cpcG2Δ59

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pINTO-N3::hTET3

Plasmid

#136364

Purpose

Expresses epitope-tagged human TET3 WT in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Tags

2xStrep-tagII, FLAG, and HA

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-USP11

Plasmid

#78849

Purpose

To purify GST-tagged recombinant USP11 protein

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2015 Sep;22(9):1463-7

Insert

USP11 (USP11 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pLentiCas9-P2A-BFP

Plasmid

#149447

Purpose

Expresses Cas9 and BFP in a lentiviral vector.

Depositor

Jonathan Chernoff

Article

Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

Insert

P2A-BFP

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

In frame with Cas9

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pRosa26-4xInsulator (JP6)

Plasmid

#99627

Purpose

IfgMosaic acceptor vector for Classical or Cas9 Gene targeting of the Rosa26 locus

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-huTET1CD-T2A-mCherry(PX458)

Plasmid

#129027

Purpose

targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA cloning site

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-MBD1

Plasmid

#47057

Purpose

Expresses methyl-CpG binding domain protein 1

Depositor

Huda Zoghbi

Article

Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

Insert

Methyl-CpG Binding Domain Protein 1 (MBD1 Human)

Use

Gateway Cloning

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
pMH4-SYN-EGFP-ER

Plasmid

#22285

Depositor

Thomas Oertner

Article

Holbro et al Proc Natl Acad Sci U S A. 2009 Sep 1;106

Insert

EGFP-ER

Tags

EGFP-ER

Expression

Mammalian

Mutation

EGFP alanine 206 changed to Lysin

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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pDN-Cherry-sgRNA

Plasmid

#221387

Purpose

episomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAs

Depositor

Deborah Hung

Article

Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

Insert

none, cloning vector for introduction of targeting sequences

Use

CRISPR

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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PP_070_pAAV_CAG_LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

Plasmid

#203228

Purpose

Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

Depositor

Adam Cohen

Article

Park et al Nat Commun. 2025 Feb 4;16(1):1333. doi:

Insert

Membrane-localized optopatch for neuronal dendrites

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCM130

Plasmid

#45828

Depositor

Mary Lidstrom

Article

Marx et al Microbiology. 2001 Aug;147(Pt 8):2065-75

Type

Empty backbone

Use

Low-background broad-host-range promoter-probe ve…

Promoter

none

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pc3.1-CAGGS-ASAP3-mCyRFP3

Plasmid

#193323

Purpose

A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3

Depositor

Michael Lin

Article

Kim et al Sci Rep. 2022 Mar 7;12(1):3678. doi: 10.

Insert

ASAP3-R3

Expression

Mammalian

Promoter

caggs

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only
plenti-X1-Neo-RPN1-HA

Plasmid

#139868

Purpose

Lentiviral expression of HA-tagged (C terminal) human RPN1

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

RPN1 (Ribophorin1) (RPN1 Human)

Use

Lentiviral

Tags

HA

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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GW1-mito-mCherry-roGFP2

Plasmid

#107220

Purpose

Mammalian expression plasmid to target the mCherry-roGFP2 sensor to the mitochondrial matrix.

Depositor

Mathew Tantama

Article

Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

Insert

mCherry-roGFP2

Tags

4xCox8

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-WIF1

Plasmid

#99533

Purpose

WIF1 expression plasmid

Depositor

Qian Tao

Article

Chan et al Lab Invest. 2007 Jul;87(7):644-50. Epub

Insert

WIF1 (WIF1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-WDR5

Plasmid

#59969

Purpose

Bacterial Expression GST-WDR5

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 (WDR5 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only
pHM6g.AQ_1489

Plasmid

#11539

Depositor

Sung-Hou Kim

Article

Liu et al Proteins. 2003 Nov 1. 53(2):326-8.

Insert

tRNA methyltransferase

Tags

HisTag-MBP, TEV cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pGAL4Kan

Plasmid

#32320

Depositor

Steve Stowers

Article

Stowers et al Fly (Austin). 2011 Oct 1;5(4).

Insert

GAL4 Kan

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(4/5FTrp)RS

Plasmid

#207620

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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