We narrowed to 7,128 results for: Trac;

1. pSEM255 - sgRNA mosTI Pmlc-2_GFP

   Plasmid

   #159835

   Purpose

   Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI Pmlc-2_GFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. FRB_LD 0_CS G_tTA-BFP

   Plasmid

   #58876

   Purpose

   MESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
   

   Use

   AAV

   Tags

   EBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. σ1-mCherry

   Plasmid

   #186578

   Purpose

   Expression of adaptor protein 1 (AP1) sigma subunit AP1S1 tagged with mCherry

   Depositor

   Stephen Royle

   Article

   Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

   Insert

   AP1S1 (AP1S1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. ECFP-ER-0

   Plasmid

   #55342

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   ER (CALR Human)
   

   Use

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   ER Signal Peptide

   Promoter

   CMV

   Available since

   Oct. 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

5. 3XFlagNICD4

   Plasmid

   #20186

   Depositor

   Raphael Kopan

   Article

   Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

   Insert

   Notch 4 Intracellular Domain (Notch4 Mouse)
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Contains murine Notch 4 Intracellular Domain (Val…

   Promoter

   CMV

   Available since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pECIA2

   Plasmid

   #47032

   Purpose

   This is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.

   Depositor

   Chris Garcia

   Article

   Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

   Type

   Empty backbone

   Use

   Secreted expression in drosophila culture

   Tags

   Fc(human IgG1), HRV 3C Protease Cleavage site, He…

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein (Copper-Inducible)

   Available since

   Aug. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-RiboL1-jGCaMP7c

   Plasmid

   #192619

   Purpose

   AAV transfer plasmid for neuronal expression of soma-targeted (RiboL1) jGCaMP7c.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   RiboL1-jGCaMP7c
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CD200RCD4d3+4-bio-linker-His

   Plasmid

   #36153

   Purpose

   A positive control receptor bait with bio-linker-His (rat CD4d3+4-bio-linker-his) and prey (rat CD200 receptor) EXPRESs plasmid

   Depositor

   Gavin Wright

   Article

   Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

   Insert

   CD200R (Cd200r1 Rat)
   

   Use

   Tags

   His tag, biotinylation peptide, and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPomyces-1

   Plasmid

   #61736

   Purpose

   Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA

   Depositor

   Huimin Zhao

   Article

   Cobb et al ACS Synth Biol. 2014 Dec 8.

   Inserts

   sSpCas9
   tracrRNA
   crRNA cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   BbsI-flanked lacZ cassette inserted in place of s…

   Promoter

   gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI

   Available since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-mNeonGreen-LEHD-NanoLuc

    Plasmid

    #98289

    Purpose

    caspase-9 BRET Sensor

    Depositor

    Maarten Merkx

    Article

    den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

    Insert

    mNeonGreen-LEHD-NanoLuc
    

    Use

    Tags

    His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-GCaMP3bright

    Plasmid

    #74423

    Purpose

    Express fluorescent calcium indicator GCaMP3bright in mammalian cells

    Depositor

    Katalin Torok

    Article

    Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

    Insert

    GCaMP3bright
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D392A

    Promoter

    CMV

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. cyto-YFP-FKBPx5

    Plasmid

    #103777

    Purpose

    expression of iPOLYMER component in mammalian cells; has nuclear exclusion sequence

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    cyto-YFP-FKBPx5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. dmBACCS2-IRES-dOrai

    Plasmid

    #67627

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.GFAP.(cyto).iATPSnFR2.S29W.A95K.HaloTag

    Plasmid

    #209659

    Purpose

    Expression of iATPSnFR2, high affinity

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.S29W.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S29W.A95K

    Promoter

    GFAP

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. cyto-CFP-FRBx5

    Plasmid

    #103776

    Purpose

    expresses iPOLYMER component in mammalian cells

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    cyto-CFP-FRBx5
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-SRGAP1

    Plasmid

    #187269

    Purpose

    Expression of SRGAP1-EGFP

    Depositor

    Alpha Yap

    Article

    Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

    Insert

    SRGAP1 (SRGAP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. GRK2 S670A

    Plasmid

    #45755

    Depositor

    Robert Lefkowitz

    Article

    Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

    Insert

    GRK2 S670A (GRK2 Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S670A

    Promoter

    CMV

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pEGFP-N1-human lyn–GFP

    Plasmid

    #35958

    Purpose

    Expresses human lyn protein codon optimized for zebrafish expression.

    Depositor

    Anna Huttenlocher

    Article

    Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

    Insert

    LYN (LYN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    cmv

    Available since

    April 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.GFAP.(cyto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209661

    Purpose

    Expression of iATPSnFR2, low affinity

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    A95A.A119L

    Promoter

    GFAP

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. IMS-HyPer2

    Plasmid

    #60248

    Purpose

    Genetically-encoded fluorescent hydrogen peroxide indicator HyPer2 targeted to mitochondrial intermembrane spase.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    IMS-HyPer2
    

    Use

    Tags

    Mitochondrial intermembrane space targeting signa…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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