We narrowed to 4,335 results for: U6 gRNA

1. pKLV2-U6gRNA5(gKAT7-A10)-PGKpuro2ABFP-W

   Plasmid

   #159288

   Purpose

   Lentiviral vector expressing gRNA targeting KAT7 (gRNA ID. A10)

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   guide RNA targeting KAT7 (ID. A10) (KAT7 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC368: pAAV.CMV-Cas13e-VEGFA sgRNA2

   Plasmid

   #227800

   Purpose

   Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA2

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA 2 (Vegfa Synthetic)
   Cas13e
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC369: pAAV.CMV-Cas13e-VEGFA sgRNA3

   Plasmid

   #227801

   Purpose

   Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA3

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA 3 (Vegfa Synthetic)
   Cas13e
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2ABFP-W

   Plasmid

   #163175

   Purpose

   Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   WWTR1 (WWTR1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2ABFP-W

   Plasmid

   #163174

   Purpose

   Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   WWTR1 (WWTR1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 330-cherry-hCYP3A4-enhancer-L-sgRNA

   Plasmid

   #176840

   Purpose

   Targeting human CYP3A4 proximal enhancer left boundary. sgRNA expressing cells could be FACS sorted by cherry expression.

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   Human CYP3A4 proximal enhancer left boundary
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2ABFP-W

   Plasmid

   #163170

   Purpose

   Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   YAP1 (YAP1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2AmCherry-W

   Plasmid

   #163172

   Purpose

   Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   YAP1 (YAP1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV CMV-DIO-(mCherry-U6)-shRNA(scrambled)

   Plasmid

   #214733

   Purpose

   Expresses mCherry protein in infected and cre positive cells

   Depositor

   Félix Leroy

   Article

   de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

   Insert

   anti-Crh shRNA (scrambled) / mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-U6gRNA5(gKAT6A-A8)-PGKpuro2ABFP-W

    Plasmid

    #159290

    Purpose

    Lentiviral vector expressing gRNA targeting KAT6A (gRNA ID. A8)

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    guide RNA targeting KAT6A (ID. A8) (KAT6A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV2-U6gRNA5(gKAT6A-A7)-PGKpuro2ABFP-W

    Plasmid

    #159289

    Purpose

    Lentiviral vector expressing gRNA targeting KAT6A (gRNA ID. A7)

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    guide RNA targeting KAT6A (ID. A7) (KAT6A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA

    Plasmid

    #113691

    Purpose

    U6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA

    Plasmid

    #113692

    Purpose

    U6 SaCas9 gRNA expression cassette containing a gRNA targeting the CREB gene, followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Inserts

    SaCas9 (NEWENTRY )
    SaCas9 gRNA Cassette (NEWENTRY )
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPRE

    Plasmid

    #176273

    Purpose

    Expression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI sgRNA (sgTet) and BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. PiggyBac EF1a-eGFP-Puro U6 PPP2CA g2

    Plasmid

    #170823

    Purpose

    PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    Cas13d PPP2CA gRNA2
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PiggyBac EF1a-Tet-on 3G-Puro U6 BbsI

    Plasmid

    #159756

    Purpose

    PiggybacTransposon-based expression control of Tet-on 3G, Puro and Cas13d U6 gRNA backbone

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR221-H1-sgGFP1-U6-sgGFP2-7SK-sgGFP3

    Plasmid

    #87906

    Purpose

    sgRNA targeting GFP

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    sgGFP1, sgGFP2, sgGFP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1, U6, 7SK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. XLone-Puro Cas13d-eGFP U6 SOX17 g2

    Plasmid

    #155188

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA2

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    Cas13d SOX17 gRNA2
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS/U6/RNAi gamma-tubulin shRNA mouse

    Plasmid

    #70666

    Purpose

    Reduced the expression of gamma-tubulin in murine cells

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    5´-GGCTCATGATGGCCAACCACCAAGCTTGGTGGTTGGCCATCATGAGCCCTTTTTC-3´ 5´-CCGAGTACTACCGGTTGGTGGTTCGAACCACCAACCGGTAGTACTCGGGAAAAAGTTAA-3´ (Tubg1, Tubg2 Mouse)
    

    Use

    U6 promoter

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-U6(BsmBI)-EF1a-BFP-Nkx2-5

    Plasmid

    #117135

    Purpose

    Expression vector for Nkx2-5 and gRNA

    Depositor

    Kosuke Yusa

    Article

    Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

    Insert

    Nkx2-5 CDS (Nkx2-5 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    modified BFP

    Promoter

    human U6 promoter, human EF1a promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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