We narrowed to 4,348 results for: ado

1. JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)

   Plasmid

   #156405

   Purpose

   Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codon

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human wild type KRAS4A-WT (KRAS Human)
   

   Use

   Gateway cloning

   Tags

   mScarlet-I

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4.23 C3_29

   Plasmid

   #60309

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   ZFP36L2 enhancer (ZFP36L2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pENTR D-TOPO-C3_10

   Plasmid

   #60259

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   RFX6 enhancer (RFX6 Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR D-TOPO-C3_25

   Plasmid

   #60268

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   ROCK1 enhancer (ROCK1 Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ai14-luc

   Plasmid

   #87118

   Purpose

   AAV backbone plasmid including luc-pA knock-in donor and Ai14gRNA for HITI

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   U6-Ai14sgRNA-Luciferase-nEF-GFPKASH-hGHpA
   

   Use

   AAV, CRISPR, and Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4.23 C3_31

   Plasmid

   #60310

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   SLC2A2 enhancer (SLC2A2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pGL4.23 C3_21

   Plasmid

   #60296

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   CDKN2BAS enhancer (CDKN2B-AS1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pGL4.23 C3_19

   Plasmid

   #60302

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   THADA enhancer (THADA Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pET28b-Hf-RfxCas13d-His

   Plasmid

   #234480

   Purpose

   Plasmid for bacterial expression and purification of High-fidelity RfxCas13d protein (human codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

   Insert

   RfxCas13d
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Changed four alanine to valine in positions 134, …

   Promoter

   Available since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-multi-CRISPR-sgRipk1_#1-puro

    Plasmid

    #231984

    Purpose

    Knockout mouse Ripk1

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-multi-CRISPR-sgRipk1_#2-puro

    Plasmid

    #231983

    Purpose

    Knockout mouse Ripk1

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-HNF1B-FUS-IDR

    Plasmid

    #183240

    Purpose

    Expression of HNF1B fused to a intrinsically disordered regions of the FUS protein

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    HNF1 homeobox B (HNF1B Human)
    

    Use

    Tags

    Fusion to an IDR from the FUS protein and FLAG ta…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-HNF1A-delta-IDR2

    Plasmid

    #183238

    Purpose

    Expression of FLAG tagged HNF1A with deletion of a intrinsically disordered regions (AA545-573)

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    HNF1 homeobox A (HNF1A Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 545-573

    Promoter

    CMV

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGFP-nE-mHP1alpha

    Plasmid

    #181895

    Purpose

    Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    nE-mHP1alpha (Cbx5 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E

    Promoter

    CMV

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGFP-nE-mHP1alpha-delCTE

    Plasmid

    #181896

    Purpose

    Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates, C-terminal deletion

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    nE-mHP1alpha-delCTE (Cbx5 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGFP-FUSN-mHP1alpha

    Plasmid

    #181897

    Purpose

    Fluorescently tagged HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN)

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    FUSN-mHP1alpha (Cbx5 Mouse, Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGFP-PGL-1-mHP1alpha

    Plasmid

    #181899

    Purpose

    Fluorescently tagged HP1alpha fused to PGL-1

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PGL-1-mHP1alpha (Cbx5 Mouse, Nematode)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRGG-GFP-RGG-mHP1alpha

    Plasmid

    #181898

    Purpose

    Fluorescently tagged HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    RGG-GFP-RGG (Cbx5 Mouse, Nematode)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-PGK-flag-RAP1B-N17-puro

    Plasmid

    #156169

    Purpose

    Lentiviral vector for expression of flag-RAP1B-N17, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1B (RAP1B Bovine)
    

    Use

    Lentiviral

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    changed Serine 17 to Asparagine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-PGK-RAP2A-WT-puro

    Plasmid

    #156170

    Purpose

    Lentiviral vector for expression of RAP2A-WT, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    wild type form

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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