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pHA-ΔN331-Kin1B

Plasmid

#31603

Depositor

Anthony Brown

Article

Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

Insert

ΔN331 kinesin-1B (Kif5b Mouse)

Tags

HA-tag

Expression

Mammalian

Mutation

deleted amino acids 1-331

Promoter

CMV

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pRU52

Plasmid

#167678

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUB4-2::spCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRMH120

Plasmid

#40593

Purpose

Expresses Gal4BD-CRY2PHR and Gal4AD-CIB1 for light-inducible protein expression

Depositor

Chandra Tucker

Article

Hughes et al Methods. 2012 Aug 15.

Inserts

CRY2 (CRY2 Mustard Weed)
CIB1 (CIB1 Mustard Weed)

Expression

Yeast

Promoter

ADH1 and TEF

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pRU51

Plasmid

#167677

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUB4-2::spCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM28521

Plasmid

#105359

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

EC1.2en-EC1.1p fusion promoter

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pGBKT7-RmMYB108

Plasmid

#173183

Purpose

Expresses Rosa multiflora MYB108 for transcriptional activation studies in yeast.

Depositor

Daidi Che

Article

Dong et al Front Plant Sci. 2021 Jul 27;12:696919.

Insert

RmMYB108

Expression

Yeast

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pHW-CIBN-MP-HRW-CRY2-VHH(GFP)

Plasmid

#126024

Purpose

express RFP-tagged LARIAT for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-MP; CRY2 PHR-VHH(GFP)

Tags

RFP

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAL189

Plasmid

#22278

Purpose

Expresses Intersectin DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

IntersectinDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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OSCA1.2-PP-GFP

Plasmid

#135390

Purpose

Expresses OSCA1.2 in mammalian cells

Depositor

Ardem Patapoutian

Article

Jojoa-Cruz et al Elife. 2018 Nov 1;7. pii: 41845. doi: 10

Insert

OSCA1.2 (AT4G22120 Mustard Weed)

Tags

EGFP tag connected to the gene via a precision pr…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pETM6-At4CL-CmCHS-CmCHI

Plasmid

#73395

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
CmCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pJOG603

Plasmid

#105356

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

promotor RPS5a (At3g11940; 1700bp)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSumo-His6- Sumo-AtTPL(1-209)

Plasmid

#177856

Purpose

Bacterial Expression of TPL

Depositor

Karsten Melcher

Article

Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

Insert

TPL (TPL Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHA-ΔN332-Kin1C

Plasmid

#31608

Depositor

Anthony Brown

Article

Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

Insert

ΔN332 kinesin-1C (Kif5c Mouse)

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pR540

Plasmid

#207885

Purpose

template for 2 gRNA amplification

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

scaffold-pU6

Expression

Bacterial

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCS021

Plasmid

#207917

Purpose

Level 0 for gRNA in E2 module (5' CCAT-3' ACTA)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pKE013

Plasmid

#207916

Purpose

Level 0 for gRNA in E1 module (5' CTGC-3' CCAT)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBIP1::eYFP

Plasmid

#135231

Purpose

UPR signaling reporter (upregulates eYFP expression under UPR conditions)

Depositor

Armin Djamei

Article

Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

Insert

pBiP1

Tags

eYFP

Expression

Plant

Promoter

BiP1

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSA10(gfcB-His-CD)

Plasmid

#176194

Purpose

expresses 3 overlapping genes (gfcB-6xHis, gfcC, gfcD) from this one operon

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcBCD

Tags

GfcB-6xHis

Expression

Bacterial

Promoter

Ptac

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBH31(gfcD)

Plasmid

#176199

Purpose

Encodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasm

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Tags

pelC leader-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBH31(gfcD-10xHis)

Plasmid

#176200

Purpose

Encodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasm

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Tags

pelC leader-10xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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