We narrowed to 8,683 results for: sgRNA

1. pIKU801-CrPEPC1

   Plasmid

   #92342

   Purpose

   Expresses sgRNA for targeting CrPEPC1 gene, paromomycin resistance

   Depositor

   I-Son Ng

   Article

   Kao et al Bioresour Technol. 2017 May 4. pii: S096

   Insert

   sgRNA-CrPEPC1
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtU6-26

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTarget12f1

   Plasmid

   #171183

   Purpose

   Constitutive expression of sgRNA without donor DNA

   Depositor

   Kohsuke Honda

   Article

   Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

   Insert

   sgRNA scaffold in the CRISPR/Cas12f1 system
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119(SpeI) promoter

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-TO-MCP-3XBFPnls

   Plasmid

   #75383

   Purpose

   MCP-3XBFPnls

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   MCP
   

   Use

   Lentiviral

   Tags

   3XBFP

   Expression

   Mammalian

   Promoter

   CMV-TO

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX459_gRNA-AAVS1_hspCas9

   Plasmid

   #193309

   Purpose

   Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   not applicable

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFD3-frame_selector_0

   Plasmid

   #127553

   Purpose

   Drosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-in

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   sgRNA frame 0
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   dU6:3

   Available Since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFD3-frame_selector_1

   Plasmid

   #127554

   Purpose

   Drosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-in

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   sgRNA frame 1
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   dU6:3

   Available Since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFH102

   Plasmid

   #128207

   Purpose

   sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. LBJJ382

   Plasmid

   #117518

   Purpose

   Also known as LBJJ429. PCR template for sgRNA-EF with polyT, 67bp or 192bp terminator

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:ACTA:pU6-26:sgRNA-EF:t192bp:TTAC:BpiI
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtU6-26

   Available Since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAK005

   Plasmid

   #128218

   Purpose

   improved sgRNA backbone for tRNA-gRNA array, Position 5

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for tRNA-gRNA array, Position 5
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAK003

    Plasmid

    #128216

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 3

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 3
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAK004

    Plasmid

    #128217

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 4

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 4
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAK006

    Plasmid

    #128219

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 6

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 6
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAK002

    Plasmid

    #128215

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 2

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 2
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAK008

    Plasmid

    #128225

    Purpose

    classic sgRNA backbone for tRNA-gRNA array, Position 2

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    classic sgRNA backbone for tRNA-gRNA array, Position 2
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAK009

    Plasmid

    #128226

    Purpose

    classic sgRNA backbone for tRNA-gRNA array, Position 3

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    classic sgRNA backbone for tRNA-gRNA array, Position 3
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCas9-J23109

    Plasmid

    #113150

    Purpose

    Plasmid constitutively expressing Cas9 protein

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Insert

    Cas9
    

    Expression

    Bacterial

    Available Since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX459_gRNA pAS single_hspCas9

    Plasmid

    #193311

    Purpose

    Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    not applicable

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pgRNA-guideless

    Plasmid

    #129464

    Purpose

    Derived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Guideless gRNA backbone
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    BBa_J23119 (SpeI)

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3s-Cas9-HF1

    Plasmid

    #104173

    Purpose

    Cas9-HF1 mammalian expression

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

    Insert

    Cas9-HF1
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    CMV

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. p3s-eCas9-1.1

    Plasmid

    #104172

    Purpose

    eCas9-1.1 mammalian expression

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

    Insert

    eCas9-1.1
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    CMV

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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