We narrowed to 3,040 results for: multiplex

1. pAAV-hSynapsin1-RdLight1

   Plasmid

   #125708

   Purpose

   Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian neurons.

   Depositor

   Lin Tian

   Article

   Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

   Has Service

   AAV9

   Insert

   RdLight1
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mutation

   Promoter

   hSynapsin1

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMVp-dsRed2-Triplex-28-gRNA1-28-pA

   Plasmid

   #55200

   Purpose

   Plasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   dsRed2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ-dSpRY-Act3.0

   Plasmid

   #158416

   Purpose

   CRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dSpRY-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJOG1368

   Plasmid

   #167301

   Purpose

   Level 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-Fluorocytosine

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Insert

   FCY-UPP fusion gene
   

   Use

   Synthetic Biology; Moclo level 0 vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMax_mOrange_A215C

   Plasmid

   #177828

   Purpose

   Site-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).

   Depositor

   Zachary Nagel, Leona Samson

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   mOrange_A215C
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A215C

   Promoter

   CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-RdLight1

   Plasmid

   #125707

   Purpose

   Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.

   Depositor

   Lin Tian

   Article

   Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

   Has Service

   AAV9

   Insert

   dLight1.3b
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJOG1336

   Plasmid

   #167303

   Purpose

   Level 0 module (3U+Ter) for further assemblies based on the Modular Cloning system. 3'UTR and flanking sequence of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Insert

   3'UTR and Terminator SlOLE2
   

   Use

   Synthetic Biology; Moclo level 0 vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJOG1334

   Plasmid

   #167302

   Purpose

   Level 0 module (Pro+5U) for further assemblies based on the Modular Cloning system. Promoter and 5'UTR of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Insert

   Promoter SlOLE2
   

   Use

   Synthetic Biology; Moclo level 0 vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. FASTHDR-Cterm-mTagBFP2-Blasticidin

   Plasmid

   #167207

   Purpose

   Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. SSTR3-DuET

    Plasmid

    #213378

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    SSTR3 (SSTR3 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. dpYPQ230-D156R-CBE4

    Plasmid

    #195369

    Purpose

    dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

    Insert

    hA3A/Y130F-(GGGGS)x6-dLbCas12a-D156R-UGI
    

    Use

    CRISPR; Gateway compatible ha3a/y130f-(ggggs)x6-d…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    ZmUBI1

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMax_GFP_C289T

    Plasmid

    #177826

    Purpose

    Site-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    GFP_C289T
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C289T

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. dpYPQ230-D156R-ABE5

    Plasmid

    #195371

    Purpose

    dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x8

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

    Insert

    ecTadA8e-(GGGGS)x8-dLbCas12a-D156R
    

    Use

    CRISPR; Gateway compatible ectada8e-(ggggs)x8-dlb…

    Tags

    5' and 3' NLS

    Expression

    Plant

    Mutation

    Promoter

    ZmUBI1

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3xFlag-tdMCP-Erk2 (pErk reporter)

    Plasmid

    #226167

    Purpose

    Used to transiently express the pErk reporter in LABEL-seq MAPK activity assays.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Insert

    3xFlag-tdMCP-Erk2 (MAPK1 Human)
    

    Use

    Tags

    3xFlag-tdMCP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJC-repo1.9kb-TALE4-VP64-P10

    Plasmid

    #104612

    Purpose

    Expresses TALE4 under the control of a 1.9kb repo enhancer element

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    1.9kb repo enhancer element (repo Fly)
    

    Use

    TALEN

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-CAG::Cytbow

    Plasmid

    #158995

    Purpose

    Multicolor cytoplasmic cell labeling with a genome-integrating piggyBac transposon vector (randomly expressing tdTomato, mTurquoise2 or mEYFP upon Cre recombination)

    Depositor

    Jean Livet

    Article

    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

    Insert

    Cytbow
    

    Use

    Tags

    The default expressed EBFP2 is fused to H2B

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. S1PR1-DuET

    Plasmid

    #213374

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    S1PR1 (S1PR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBUN411

    Plasmid

    #50581

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    3×FLAG-NLS-zCas9-NLS
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    maize codon optimized

    Promoter

    OsU3p and Ubi1p

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMax_BFP_A191G

    Plasmid

    #177823

    Purpose

    Site-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    tagBFP_A191G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A191G

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMax_mPlum_C207G/T208C

    Plasmid

    #177831

    Purpose

    Site-specific mutagenesis of mPlum for the purposes of creating a site-specific O6-MeG lesion for the purposes of measuring MGMT activity via Host Cell Reactivation (HCR).

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    mPlum_C207G/T208C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C207G + T208C

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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