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BB1_12_pMDH3

Plasmid

#98503

Purpose

BB1 with insert: promoter PMDH3

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

MDH3 promoter

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_12_pADH2

Plasmid

#98504

Purpose

BB1 with insert: promoter PADH2

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

ADH2 promoter

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_12_pFBA1-1

Plasmid

#98505

Purpose

BB1 with insert: promoter PFBA1-1

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

FBA1-1 promoter

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_12_pSHB17

Plasmid

#98506

Purpose

BB1 with insert: promoter PSHB17

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

SHB17 promoter

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_12_pTEF2

Plasmid

#98507

Purpose

BB1 with insert: promoter PTEF2

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

TEF2 promoter

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_12_pGUT1

Plasmid

#98511

Purpose

BB1 with insert: promoter PGUT1

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

GUT1 promoter

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_12_pTHI11

Plasmid

#98512

Purpose

BB1 with insert: promoter PTHI11

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

THI11 promoter

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_12_pFDH1

Plasmid

#98516

Purpose

BB1 with insert: promoter PFDH1

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

FDH1 promoter

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_34_ScCYC1tt

Plasmid

#98519

Purpose

BB1 with insert: terminator ScCYC1tt

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

ScCYC1tt

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_34_TDH3tt

Plasmid

#98520

Purpose

BB1 with insert: TDH3tt

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

TDH3tt

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_34_RPS2tt

Plasmid

#98521

Purpose

BB1 with insert: RPS2tt

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

RPS2tt

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_34_RPP1Btt

Plasmid

#98522

Purpose

BB1 with insert: RPP1Btt

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

RPP1Btt

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_34_RPS17Btt

Plasmid

#98523

Purpose

BB1 with insert: RPS17Btt

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

RPS17Btt

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_12_pGPM1

Plasmid

#98499

Purpose

BB1 with insert: promoter PGPM1

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

GPM1 promoter

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB1_12_pRPP1B

Plasmid

#98500

Purpose

BB1 with insert: promoter PRPP1B

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

RPP1B promoter

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_12_pPDC1

Plasmid

#98501

Purpose

BB1 with insert: promoter PPDC1

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

PDC1 promoter

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_12_pPOR1

Plasmid

#98502

Purpose

BB1 with insert: promoter PPOR1

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

POR1 promoter

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFP88

Plasmid

#83544

Purpose

Used to evaluate the expression output of RPL15A promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL15A promoter-yEGFP

Expression

Yeast

Promoter

RPL15A

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP77

Plasmid

#83538

Purpose

Used to evaluate the expression output of RPL8B promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL8B promoter-yEGFP

Expression

Yeast

Promoter

RPL8B

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFPH0

Plasmid

#83552

Purpose

Used to evaluate the expression output of TPS1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TPS1 promoter-yEGFP

Expression

Yeast

Promoter

TPS1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFP90

Plasmid

#83547

Purpose

Used to evaluate the expression output of TEF2 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF2 promoter-yEGFP

Expression

Yeast

Promoter

TEF2

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFP82

Plasmid

#83541

Purpose

Used to evaluate the expression output of YEF3 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

YEF3 promoter-yEGFP

Expression

Yeast

Promoter

YEF3

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFP80

Plasmid

#83540

Purpose

Used to evaluate the expression output of RPL3promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL3 promoter-yEGFP

Expression

Yeast

Promoter

RPL3

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFP78

Plasmid

#83539

Purpose

Used to evaluate the expression output of SSB1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

SSB1 promoter-yEGFP

Expression

Yeast

Promoter

SSB1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL3095

Plasmid

#41269

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-her6-Right (her6 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only
TAL3094

Plasmid

#41268

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-her6-Left (her6 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00364

Plasmid

#34480

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG30020 (CG30020 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
Neurl1-TOPO

Plasmid

#17281

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pET SUMO NL-FKBP-Cas9

Plasmid

#162730

Purpose

Bacterial expression, protein expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Tags

6xHis and SUMO

Expression

Bacterial

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
UG76-pLYS2-GFP-PKCbeta

Plasmid

#184762

Purpose

Marker for DAG. Yeast expression of human PKC beta under LSY2 promoter. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

pLYS2-GFP-PKCbeta (PRKCB Human, Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCXWB-EBNA1

Plasmid

#37624

Purpose

Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

EBNA-1 (EBNA-1 human herpesvirus 4)

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pYL156 (TRV RNA2)

Plasmid

#148969

Purpose

Modified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)

Depositor

Savithramma Dinesh-Kumar

Article

Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

Insert

Modified TRV RNA2

Use

T-dna vector

Mutation

see comments section below

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBR-Cas9ΔyqhD-HA500

Plasmid

#220369

Purpose

A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

Depositor

Yilan Liu

Article

Wen et al Microb Cell Fact. 2024 Dec 30;23(1):349.

Inserts

SpCas9
sgRNA for yqhD in P. putida KT2440

Use

CRISPR; Broad host range

Expression

Bacterial

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP

Plasmid

#215055

Purpose

Expresses EGFP in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

EGFP

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBTK614

Plasmid

#110608

Purpose

BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

dCas9

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Human_TFAM_NoMTS_pET28

Plasmid

#34705

Depositor

David Chan

Article

Ngo et al Nat Struct Mol Biol. 2011 Oct 30;18(11):

Insert

TFAM (TFAM Human)

Tags

6XHis

Expression

Bacterial

Mutation

Encodes residues 43–246, corresponding to full-le…

Promoter

T7

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
px330_Rosa_sgRNA

Plasmid

#97007

Purpose

Expresses Cas9 and Rosa26 locus specific sgRNA

Depositor

Russell Ray

Article

Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

Insert

Rosa26 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Flag-full length mSesn2 (FL: A+B+C)

Plasmid

#111796

Purpose

Mammalian Expression of mSesn2

Depositor

Jun Hee Lee

Article

Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

Insert

mSesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Full length

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p-eGFP-beta 2 CP

Plasmid

#13298

Depositor

John Cooper

Article

Schafer et al J Cell Biol. 1998 Dec 28. 143(7):1919-30

Insert

capping protein beta 2 subunit (Capzb Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pLDLR-Luc (aka: pES7)

Plasmid

#14940

Depositor

Axel Nohturfft

Article

Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

LDLR promoter (LDLR Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

May 21, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM705 Gs

Plasmid

#109350

Purpose

Wildtype Gαs

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

GαS (GNAS Human)

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-V5-KDM3A

Plasmid

#82331

Purpose

lentiviral expression of KDM3A

Depositor

Luke Gaughan

Article

Wade et al Nucleic Acids Res. 2015 Jan;43(1):196-20

Insert

KDM3A (KDM3A Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

two silent mutations to make it siRNA resistant

Available Since

March 14, 2017

Availability

Academic Institutions and Nonprofits only
ELF1_pet28a

Plasmid

#131649

Purpose

Bacterial expression of ETS gene ELF1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF1 (ELF1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLC-242

Plasmid

#73189

Purpose

Template plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

Depositor

Swaine Chen

Article

Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

Insert

chloramphenicol (cat Although Ampicillin gene is present, it was inactivated while cloning.)

Expression

Bacterial

Promoter

chloramphenicol promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-HA3-HPS1

Plasmid

#133930

Purpose

Expresses 3xHA-HPS1 construct in mammalian cells

Depositor

Juan Bonifacino

Article

Martina et al J Biol Chem. 2003 Aug 1;278(31):29376-84

Insert

HPS1 (HPS1 Human)

Tags

3xHA tag

Expression

Mammalian

Promoter

CMV I.E.

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.11-Cd36-promoter

Plasmid

#138572

Purpose

Firefly luciferase reporter fused with a 1296 bp fragment of mouse Cd36 promoter

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Promoter

SV40

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBR_Cas9_sp1

Plasmid

#154851

Purpose

Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome

Depositor

John van der Oost

Article

Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.

Inserts

Spcas9 gene
sgRNA scaffold

Use

CRISPR

Tags

6xHis

Mutation

Codon harmonized variant for expression in Rhodob…

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Neurl1-Flag pcDNA3.1

Plasmid

#17282

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pQHA-USP11 WT puroR

Plasmid

#46749

Purpose

Retroviral vector that expresses wild type HA-tagged wild type human USP11

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP11 (USP11 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
1432 pcDNA3 flag MAGI1c

Plasmid

#10714

Depositor

William Sellers

Article

Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

Insert

MAGI-1 (Magi1 Mouse)

Tags

flag

Expression

Mammalian

Available Since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 5,812 results for: 129