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Showing: 5201 - 5250 of 33749 results

  1. pelB-MBP-DipA-ss

    Plasmid
    #137068
    Purpose
    E. coli expression clone (T7lac promoter) for mature DipA (aa 21-342) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature DipA: lacks the DipA signal peptide
    Promoter
    T7lac
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137068-plasmid-map-sequence-id-291958

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  2. pelB-MBP-RevA-ss

    Plasmid
    #137064
    Purpose
    E. coli expression clone (T7lac promoter) for mature RevA (aa 25-160) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAF07416.1 (revA Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    lacks the RevA signal peptide and 5 residues (CKA…
    Promoter
    T7lac
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137064-plasmid-map-sequence-id-291953

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  3. pJW1592

    Plasmid
    #121059
    Purpose
    GFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    GFP^SEC^BioTag::AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…
    Expression
    Worm
    Available Since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    121059-plasmid-map-sequence-id-235964

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  6. pHR-mCherry-p2A-LIC1 C-terminal half

    Plasmid
    #74603
    Purpose
    expresses LIC1 in mammalian cells; aa 389-523, untagged but cells indicate expression with diffuse mCherry
    Depositor
    Ron Vale
    Article
    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554
    Insert
    full length human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
    Use
    Lentiviral
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    amino acids 389-523 of LIC1
    Promoter
    SFFV
    Available Since
    May 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    74603-plasmid-map-sequence-id-141268

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  7. pcDNA3.1/TO-myc-BirA*-cCE

    Plasmid
    #112713
    Purpose
    For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.
    Depositor
    Daniel Schoenberg
    Article
    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1
    Insert
    Rngtt (Rngtt Mouse)
    Tags
    BirA* (BirA R118G), HIV Rev nuclear localization …
    Expression
    Mammalian
    Mutation
    Silent mutation at Arg 132 (CGT to CGC), deleted …
    Promoter
    CMV
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    112713-plasmid-map-sequence-id-217067

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  8. pJW1594

    Plasmid
    #121061
    Purpose
    TagRFP-T^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    TagRFP-T^SEC^BioTag::AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…
    Expression
    Worm
    Available Since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    121061-plasmid-map-sequence-id-235980

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  12. pCW-MRTFA-HA

    Plasmid
    #247436
    Purpose
    siRNA resistant HA-tagged mouse MRTF-A cloned into the pCW backbone (Addgene #41393, with puromycin resistance) together with an IRES GFP to indicate doxycycline-inducible expression by fluorescence.
    Depositor
    Richard Treisman
    Article
    Nielsen et al J Cell Sci. 2025 Nov 7:jcs.264444. doi:
    Inserts
    MRTF-A (Mrtfa Mouse)
    IRES2:EGFP
    Use
    Lentiviral; Dox-inducible
    Tags
    HA
    Expression
    Mammalian
    Mutation
    siRNA resistant - no amino acid mutations
    Available Since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247436-plasmid-map-sequence-id-491537

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  13. pCW-Rho(G14V)-HA

    Plasmid
    #247437
    Purpose
    HA-tagged human RhoA(G14V) cloned into the pCW backbone (Addgene #41393, with puromycin resistance) together with an IRES GFP to indicate doxycycline-inducible expression by fluorescence.
    Depositor
    Richard Treisman
    Article
    Nielsen et al J Cell Sci. 2025 Nov 7:jcs.264444. doi:
    Inserts
    RhoA(G14V) (RHOA Human)
    IRES2:EGFP
    Use
    Lentiviral; Dox-inducible
    Tags
    HA
    Expression
    Mammalian
    Mutation
    G14V mutation (constitutive active mutant)
    Available Since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247437-plasmid-map-sequence-id-490945

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  16. pET28-His6-SUMO-Lyn-SH2-AviTag

    Plasmid
    #214207
    Purpose
    Bacterial expression of SH2 domain of the Lyn kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Lyn A (LYN Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214207-plasmid-map-sequence-id-422791

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  17. pET28-His6-TEV-Grb2-SH2-mCherry-AviTag

    Plasmid
    #214231
    Purpose
    Bacterial expression of SH2 domain of the Grb2 with N-terminal TEV-cleavable 6xHis tag, C-terminal mCherry, and C-terminal AviTag for Streptavidin bead functionalization for binder selections.
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Grb2 SH2 domain (GRB2 Human)
    Tags
    6xHis, AviTag, and mCherry
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214231-plasmid-map-sequence-id-424761

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  18. pET28-His6-SUMO-Lck-SH2-AviTag

    Plasmid
    #214203
    Purpose
    Bacterial expression of SH2 domain of the Lck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Lck kinase SH2 domain (LCK Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214203-plasmid-map-sequence-id-422773

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  19. pET28-His6-SUMO-Blk-SH2-AviTag

    Plasmid
    #214204
    Purpose
    Bacterial expression of SH2 domain of the Blk kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Blk kinase SH2 domain (BLK Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214204-plasmid-map-sequence-id-422774

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  20. pET28-His6-SUMO-Hck-SH2-AviTag

    Plasmid
    #214206
    Purpose
    Bacterial expression of SH2 domain of the Hck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    p60-HCK (HCK Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214206-plasmid-map-sequence-id-422790

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  24. pGEMHE-MmCENPT-RnHFD-4NS-GFP

    Plasmid
    #237432
    Purpose
    Expresses Mus musculus CENP-T with rat HFD and 4 mouse-specific residues in positions that show signs of adaptive evolution; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)
    Depositor
    Michael Lampson
    Article
    Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e
    Insert
    Mus musculus CENP-T with Rattus norvegicus HFD and 4 mouse-specific residues in HFD (Cenpt Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    432 A to T; 463 T to I, 470 E to L, 485 L to Q in…
    Promoter
    T7
    Available Since
    June 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    237432-plasmid-map-sequence-id-474252

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  37. pTwist-SARS-CoV-2 Spike-deltaC18 BA.4 (V3G,S658N)

    Plasmid
    #212504
    Purpose
    Encodes SARS-CoV-2 variant BA.4 Spike V3G,S658N for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike BA.4 (contains third aa mutation and without N658S) (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    See Depositor Comments.
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212504-plasmid-map-sequence-id-432821

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  42. pTwist-SARS-CoV-2 Spike-deltaC18 deltaL24P25P26 A27S (TCT)

    Plasmid
    #212489
    Purpose
    Encodes SARS-CoV-2 spike L24P25P26 deletion, A27S for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike L24P25P26 deletion, A27S (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    L24P25P26 deletion, A27S
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212489-plasmid-map-sequence-id-432811

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Showing: 5201 - 5250 of 33749 results