We narrowed to 13,459 results for: 109

1. pPEPZ-sgRNAclone

   Plasmid

   #141090

   Purpose

   This vector is designed for efficient cloning of sgRNAs by Golden Gate assembly. The sgRNA insertion leads to replacement of mCherry, resulting in loss of red color of E. coli colony.

   Depositor

   Jan-Willem Veening

   Article

   Liu et al Cell Host Microbe. 2021 Jan 13;29(1):107

   Insert

   mCherry
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   p3

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDest27-mBIN1+13+17

   Plasmid

   #61097

   Purpose

   Express N-terminal GST tagged-mouse BIN1+13+17 in mammalian cells

   Depositor

   Robin Shaw

   Article

   Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

   Insert

   mouse BIN1 with exon 13 and 17, excluding exon 7, 11, 14-16
   

   Tags

   GST

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCMV7.1_3xFLAG-STK4

   Plasmid

   #172989

   Purpose

   constitutive expression of FLAG-tagged STK4

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   3xFLAG-STK4 (STK4 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJP-CoV2leader-nLuc-TSS

   Plasmid

   #175430

   Purpose

   Expresses nLuc with 5' leader of CoV2 in mammalian cells.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nLUC plus 5' CoV2 leader
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJP-HBB-nLuc

   Plasmid

   #175431

   Purpose

   Expresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nLUC plus 5' HBB leader
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEYFP-C1-hSERT

   Plasmid

   #70103

   Purpose

   mammalian expression of hSERT with N-terminal YFP fusion

   Depositor

   Harald Sitte

   Article

   Sucic et al J Biol Chem. 2010 Apr 2;285(14):10924-38

   Insert

   SERT (SLC6A4 Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFUSEss-CHIg-mG2b_M18

   Plasmid

   #105931

   Purpose

   Expression plasmid coding for heavy chain of mouse IgG2b antibody specific to antigen B of the ABO blood group system, clone M18.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

   Insert

   IgG2b (Ighg2b Mouse)
   

   Expression

   Mammalian

   Promoter

   hEF1-HTLV prom

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-Bmpr2ΔKinase

   Plasmid

   #163404

   Purpose

   Expresses BMPR-2 lacking the kinase domain in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Expression

   Mammalian

   Mutation

   The kinase domain (202-500 aa) is deleted

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSR357.24

   Plasmid

   #125109

   Purpose

   Expression of narL(1-159aa)-ydfI(153-213aa) chimera under PLtetO-1, output Promoter PydfJ115

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   narL(REC)-ydfI(DBD)159
   sfgfp
   tetR
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO-puro-tetON-shGFP

    Plasmid

    #110939

    Purpose

    Tet-inducible shRNA targeting GFP

    Depositor

    William Hahn

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

    Insert

    shGFP
    

    Use

    Lentiviral and RNAi

    Promoter

    H1/TO

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV7.1_3xFLAG-TAOK3

    Plasmid

    #172993

    Purpose

    constitutive expression of FLAG-tagged TAOK3

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-TAOK3 (TAOK3 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOD_C2616

    Plasmid

    #91090

    Purpose

    Module C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Promoter

    At7SL

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBlastR-12xLexAOp-mCherry-MiniWhite

    Plasmid

    #165917

    Purpose

    Blasticidin resistant 12xLexAOp mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable LexAOp Response Vector
    

    Use

    Synthetic Biology

    Available Since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pECFP-C1-hSERT

    Plasmid

    #70104

    Purpose

    mammalian expression of hSERT with N-terminal CFP fusion

    Depositor

    Harald Sitte

    Article

    Sucic et al J Biol Chem. 2010 Apr 2;285(14):10924-38

    Insert

    SERT (SLC6A4 Human)
    

    Tags

    ECFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmTurquoise-parkin

    Plasmid

    #110945

    Purpose

    Expresses the ubiquitin ligase parkin fused to mTurquoise

    Depositor

    Pablo Rivera-Fuentes

    Article

    Nguyen et al Org Biomol Chem. 2021 Mar 28;19(12):2681

    Insert

    E3 ubiquitin ligase parkin (PRKN Human)
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX459-sgRNA2-2-RPB1-C-term

    Plasmid

    #195109

    Purpose

    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 C-term. Puromycin selection (2A-fusion).

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    sgRNA against last exon of RPB1 (C-terminal)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pINDUCER21-puro_TAOK2

    Plasmid

    #172979

    Purpose

    doxycycline-inducible expression of TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    TAOK2 (TAOK2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX C_K nsp1 CoV2

    Plasmid

    #175516

    Purpose

    Expresses C_K for bioconjugation on nsp1

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    C_K nsp1 CoV2
    

    Tags

    GST tag

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_12xTEAD_luc2

    Plasmid

    #173004

    Purpose

    firefly luciferase reporter vector with 12x clustered TEAD binding sites

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    12xTEAD-luc2
    

    Use

    AAV and Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV7.1_3xFLAG-MOB1A

    Plasmid

    #172988

    Purpose

    constitutive expression of FLAG-tagged MOB1A

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-MOB1A (MOB1A Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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