We narrowed to 4,209 results for: abo

1. pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (E44A L47A D49A A50K Q51A; deltaWIPI2)

   Plasmid

   #223777

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   BNIP3 soluble part (BNIP3 Human)
   

   Use

   Tags

   Thrombin cleavage-GST

   Expression

   Insect

   Mutation

   E44A L47A D49A A50K Q51A; disrupts WIPI2 binding

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pcDNA3

   Plasmid

   #206093

   Purpose

   Cav2.2 calcium channel with a N-terminal GFP tag, exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   E314A, E664A, E1370A, E1658A

   Promoter

   CMV

   Available since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pCAGGS

   Plasmid

   #206098

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E314A, E664A, E1370A, E1658A

   Promoter

   CMV/B-actin

   Available since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDCAF3-IS5

   Plasmid

   #65220

   Purpose

   Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.

   Depositor

   Jeffrey Barrick

   Article

   Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.

   Inserts

   ndmA
   ndmB
   ndmC
   ndmD
   gst9
   chlR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Changed start codon from gtg to atg and Changed s…

   Promoter

   BBa_J23100

   Available since

   Jan. 8, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. PGL4.11-COL1A1mSMAD

   Plasmid

   #230917

   Purpose

   Tested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.

   Depositor

   Avi Rosenberg

   Article

   Yang et al Lab Invest. 2024 May;104(5):100336. doi:

   Insert

   Human COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase-luc2p

   Expression

   Bacterial and Mammalian

   Mutation

   COL1A1 promoter with mutant potential SMAD bindin…

   Promoter

   COL1A1

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUt-mNF-BACH1

   Plasmid

   #199219

   Purpose

   LPUtopia-7 matching RMCE donor plasmid with GFP-BACH1 Negative-Feedback circuit (mNF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 

   Depositor

   Gabor Balazsi

   Article

   Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

   Insert

   hTetR::P2A::EGFP::BACH1 (BACH1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV D2ir promoter

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUt-mPF-BACH1 Cas9-Resistant_AmpR

   Plasmid

   #199221

   Purpose

   LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Positive-Feedback circuit (mPF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 

   Depositor

   Gabor Balazsi

   Article

   Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

   Insert

   rtTA-Advanced::P2A::EGFP::BACH1 (BACH1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   silient mutation at nucleic acid 177 C changed to…

   Promoter

   tight TRE promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUt-mNF-BACH1 Cas9-Resistant

   Plasmid

   #199218

   Purpose

   LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Negative-Feedback circuit (mNF-BACH1 Cas9- resistant). Use BlastR for positive selection and HSV-TK for negative selection. 

   Depositor

   Gabor Balazsi

   Article

   Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

   Insert

   hTetR::P2A::EGFP::BACH1 (BACH1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   silient mutation at nucleic acid 177 C changed to…

   Promoter

   CMV D2ir promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKT-IDH1(R132H)-IRES-Katushka

   Plasmid

   #124257

   Purpose

   Expresses IDH1 with a R132H mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

   Insert

   Isocitrate dehydrogenase 1 (R132H) (IDH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Arginine 132 is mutated to Histidine. This mutati…

   Promoter

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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