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pQE30-His-IF2

Plasmid

#124124

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Initiation factor 2 (infB Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-GFP1-10

Plasmid

#78591

Purpose

Express mCherry-GFP1-10 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

GFP 1-10

Tags

mCherry2

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT/T0-Flag-PALB2

Plasmid

#71114

Purpose

mammalian expession of FLAG-PALB2

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pTK-Snail

Plasmid

#36976

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Snail (Snai1 Mouse)

Use

Lentiviral

Available Since

May 31, 2012

Availability

Academic Institutions and Nonprofits only
HA-merlin

Plasmid

#32836

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Merlin (NF2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only
pYpet-PBD

Plasmid

#22781

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

PAK binding domain (PAK1 Human)

Tags

Ypet

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-ERK2-MEK1_fusion

Plasmid

#39194

Depositor

Melanie Cobb

Article

Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

Insert

ERK2-MEK1 Fusion (MAP2K1, Mapk1 Rat, Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
human gamma-catenin pcDNA3

Plasmid

#16827

Depositor

Eric Fearon

Article

Caca et al Cell Growth Differ. 1999 Jun . 10(6):369

Insert

gamma-catenin (JUP Human)

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pCAG-All-in-one-hCascade

Plasmid

#134919

Purpose

Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.

Depositor

Tomoji Mashimo

Article

Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

Insert

Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCW57.1-DUX4-CA

Plasmid

#99281

Purpose

All-in-one lentivector with tet-inducible DUX4 (codon sequences altered)

Depositor

Stephen Tapscott

Article

Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

Insert

DUX4 (codon altered) (DUX4 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

codon altered

Promoter

TRE

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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AgDD

Plasmid

#78289

Purpose

create protein aggregates in living cells

Depositor

Thomas Wandless

Article

Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

Insert

NES-L106P-sfGFP (FKBP1A Human)

Tags

MLALKLAGLDI

Expression

Mammalian

Promoter

pCAG

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mRuby3-18aa-Tubulin

Plasmid

#74256

Purpose

Mammalian expression of N-terminal fusion of mRuby3 to Tubulin

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

Tubulin

Tags

mRuby3

Expression

Mammalian

Promoter

CAG and CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-UBF

Plasmid

#26672

Depositor

Sui Huang

Article

Chen et al J Cell Biol. 2001 Apr 2. 153(1):169-76.

Insert

UBF1 (UBTF Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only
pLV-Ftractin-mRuby3-p2A-mTurquoise-MLC-IRES-Blast

Plasmid

#85146

Purpose

lentiviral expression of a stoichiometric F-actin and myosin light chain reporter

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Ftractin-mRuby3-P2A-mTurquoise-MLC

Use

Lentiviral

Tags

mRuby3, mTurquoise

Promoter

EF1a

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pD40-His/V5-c-Myc

Plasmid

#45597

Depositor

Rosalie Sears

Article

Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

Insert

c-Myc (Myc Mouse)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_tBFP_PGK_mCitrine

Plasmid

#188386

Purpose

Lentiviral vector - tBFP reporter for Gal4-based SNIPRs with a constitutive mCitrine;

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

Gal4UAS_tBFP_PGK_mCitrine

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TetOn-dCas9-KRAB

Plasmid

#115545

Purpose

Doxycycline inducible dCas9-KRAB targeting construct for AAVS1 locus

Depositor

Rene Maehr

Article

Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

Type

Empty backbone

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Par6 wt

Plasmid

#11748

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

PAR6 (Pard6a Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pU6-AAVS1-5-1kbdonor

Plasmid

#234735

Purpose

PseCAST crRNA targeting AAVS1-5, with 1 kb donor transposon

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

AAVS1-5 crRNA

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pDS257

Plasmid

#34971

Purpose

Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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