We narrowed to 4,675 results for: cre encoding plasmid

1. pGP-AAV-CAG-flex-iGABASnFR2(no bind)-WPRE

   Plasmid

   #218882

   Purpose

   AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2(no bind)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   S99A F102G R168P

   Promoter

   CAG

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-AAV-CAG-flex-iGABASnFR2n-WPRE

   Plasmid

   #218881

   Purpose

   AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2n
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   S99A F104H R168P

   Promoter

   CAG

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKM464 (deletions)

   Plasmid

   #108322

   Purpose

   Orbit integrating plasmid to delete target gene.

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Insert

   Bxb1 attB site
   

   Use

   Orbit integration plasmid for creating knockouts …

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-DIO-JEDI-1P-Kv-WPRE

   Plasmid

   #202608

   Purpose

   Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   JEDI-1P-Kv
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDHJS4 AS+

   Plasmid

   #78242

   Purpose

   One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).

   Depositor

   Tao-shih Hsieh

   Article

   Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

   Inserts

   attB
   attP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pDHJS4 BS-

   Plasmid

   #78245

   Purpose

   One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).

   Depositor

   Tao-shih Hsieh

   Article

   Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

   Inserts

   attB
   attP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pDHJS4 BS+

   Plasmid

   #78244

   Purpose

   One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).

   Depositor

   Tao-shih Hsieh

   Article

   Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

   Inserts

   attB
   attP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pDHJS4 AS-

   Plasmid

   #78243

   Purpose

   One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).

   Depositor

   Tao-shih Hsieh

   Article

   Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

   Inserts

   attB
   attP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 6, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pDHJS4 AN-

   Plasmid

   #78247

   Purpose

   One of four plasmids needed to create the mismatched quadruple Holliday junction substrate (MM-QHJS).

   Depositor

   Tao-shih Hsieh

   Article

   Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

   Inserts

   attB
   attP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pDHJS4 AN+

    Plasmid

    #78246

    Purpose

    One of four plasmids needed to create the mismatched quadruple Holliday junction substrate (MM-QHJS).

    Depositor

    Tao-shih Hsieh

    Article

    Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

    Inserts

    attB
    attP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pPICZ-pre-pro-alphaf-E2-Crimson

    Plasmid

    #117658

    Purpose

    Test intracellular localisation of E2-Crimson and study the secretion efficiency

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-pro-alphaf-E2-Crimson
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    This plasmid encodes the fluorescent protein E2-C…

    Promoter

    pAOX1

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-SARS2-S-D614 (C-flag)

    Plasmid

    #156420

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS CoV2)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)

    Plasmid

    #169188

    Purpose

    To express His-SUMO-nsp12 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp12 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pK27Sumo_His-SUMO-nsp8 (SARS-CoV-2)

    Plasmid

    #169187

    Purpose

    To express His-SUMO-nsp8 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOGS539_haPD-1

    Plasmid

    #226716

    Purpose

    Plasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    High affinity PD-1 microbody
    

    Use

    Tags

    HA tag and Mating factor alpha secretion signal

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOGS539_rPD-L1nb

    Plasmid

    #226717

    Purpose

    Plasmid enabling yeast-mediated expression and secretion of recombinant PD-L1 nanobody (rPD-L1nb)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Recombinant PD-L1 nanobody
    

    Use

    Tags

    HA tag, Histidine tag, and Mating factor alpha se…

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS-SARS2-S-G614 (C-flag)

    Plasmid

    #156421

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS CoV2)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. K84M kinase-dead Nuak1-3xflag

    Plasmid

    #97219

    Purpose

    Plasmid expressing kinase-dead mutant Nuak1

    Depositor

    Huda Zoghbi

    Article

    Lasagna-Reeves et al Neuron. 2016 Oct 19;92(2):407-418. doi:

    Insert

    Nuak1 (NUAK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Kinase-dead K84M

    Promoter

    T7

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCOLADuet-1_nsp10 (SARS-CoV-2)

    Plasmid

    #169158

    Purpose

    To express SARS-CoV-2 nsp10 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Methionine

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-noSV40-humanCOL2A1enhancer

    Plasmid

    #173961

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    Enhancer region from COL2A1 gene
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    None

    Promoter

    SV40 promoter

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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