We narrowed to 2,485 results for: pcas9

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pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)

Plasmid

#159658

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

Plasmid

#159657

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Dicer_2

Plasmid

#68808

Purpose

specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of

Depositor

Constance Ciaudo

Article

Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

Insert

sgRNA mouse Dicer1

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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AAEL007584-Cas9

Plasmid

#100706

Purpose

The promoter of AAEL007584 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

Depositor

Omar Akbari

Article

Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

Inserts

AAEL007584-hspcas9-GFP
Opie2-dsRed

Use

CRISPR

Tags

GFP and dsRED

Expression

Insect

Promoter

AAEL007584 and Opie2

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pJT45_GalL_nCDA1-BE3

Plasmid

#145038

Purpose

Expresses nCDA1-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1-BE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo U6-SpAi9L-U6SpDMDL

Plasmid

#78608

Purpose

Expresses gRNAs (for SpCas9) targeting 5’ of Ai9 stop cassette and Dmd intron 22

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

U6-SpAi9L-U6SpDMDL

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only
pCRII-Topo U6-SpAi9R-U6-SpDMDR

Plasmid

#78609

Purpose

Expresses gRNAs (for SpCas9) targeting 3’ of Ai9 stop cassette and Dmd intron 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

U6-SpAi9R-U6-SpDMDR

Use

Unspecified

Promoter

U6

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only
pJT79_GalL_nCDA1Δ192-BE3

Plasmid

#145045

Purpose

Expresses nCDA1Δ192-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ192-BE3

Use

CRISPR

Expression

Yeast

Mutation

pmCDA1(1-192AA); spCas9(D10A)

Promoter

GalL

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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Px458-Cas9n-Trp73-Exon4-3sgRNA

Plasmid

#88850

Purpose

CRISPR KO of Trp73

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp73 (Trp73 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
Px458-Cas9n-Trp73-Exon4-4sgRNA

Plasmid

#88851

Purpose

CRISPR KO of Trp73

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp73 (Trp73 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
pLKO1-puro-U6-sgRNA-LRTM2

Plasmid

#69237

Purpose

U6 driven SpCas9 sgRNA expression for LRTM2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

LRTM2 sgRNA (LRTM2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pSA127 A2UCOE-Cas9-BFP

Plasmid

#249151

Purpose

Overexpression of SpCas9-BFP and blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

A2UCOE-Cas9-TagBFP

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pMTKM02

Plasmid

#233474

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-Nat selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM04

Plasmid

#233476

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-G418 selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM06

Plasmid

#233478

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-Hyg selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM08

Plasmid

#233480

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-URA selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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lentiGuideFE-mSG-Puro

Plasmid

#226522

Purpose

Cas9 guide RNA scaffold with the F+E scaffold modification with mStayGold-P2A-Puro

Depositor

Neville Sanjana

Article

Caragine et al Nat Commun. 2025 Feb 13;16(1):1611. doi:

Insert

hU6_SpCas9gRNAF+E_EF1a_mStayGold_P2A_Puro

Use

CRISPR and Lentiviral

Tags

mStayGold

Expression

Mammalian

Promoter

U6,EF1a

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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EHMT1_exon 3_gRNA

Plasmid

#228809

Purpose

CRISPR targeting of human EHMT1

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

gRNA targeting EHMT1 exon 3 (EHMT1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pECNUS4_MP

Plasmid

#228358

Purpose

ABE8e-SpCas9-NG Multiplex Acceptor clone for insertion of up to six gRNA cassettes via Golden Gate with BpiI and T4 ligase, blue-white screening

Depositor

Eunyoung Chae

Article

Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

RPS5A

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-tevopreq1-FXR1-G266E

Plasmid

#225482

Purpose

Express epegRNA for FXR1

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

epegRNA for editing FXR1-G266E (FXR1 Human)

Use

CRISPR

Promoter

hU6

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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