We narrowed to 49,484 results for: Son

1. pDD268

   Plasmid

   #132523

   Purpose

   mNG^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein, Daniel Dickinson

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   mNG-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized mNG

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter

   Plasmid

   #61762

   Purpose

   Dual Color betaGlobin reporter to study splicing kinetics in vivo

   Depositor

   Daniel Larson

   Article

   Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

   Insert

   b-Globin
   

   Use

   Tags

   24 X MS2 hairpin cassette in 3'UTR of b-Glob…

   Expression

   Mammalian

   Mutation

   Promoter

   Dox inducible Tet-ON promoter

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Akaluc-N1

   Plasmid

   #120371

   Purpose

   Akaluc luciferase mammalian expression in -N1 backbone

   Depositor

   Jordan Green

   Article

   Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

   Insert

   Akaluc (human optimized)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Human codon optimized

   Promoter

   CMV

   Available since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDonor_RB-TnV2

   Plasmid

   #213907

   Purpose

   Barcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sites

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   mariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMS79 (eft-3p::Cas9 + sgRNA)

   Plasmid

   #154839

   Purpose

   Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

   Depositor

   Patrick Phillips

   Article

   Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+) Drp1 V2

   Plasmid

   #44599

   Depositor

   David Chan

   Article

   Loson et al Mol Biol Cell. 2013 Jan 2.

   Insert

   Drp1 variant 2 (Dnm1l Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. Nme2sgRNA_pLKO

   Plasmid

   #119926

   Purpose

   Nme2Cas9 U6-driven sgRNA cassette

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   NmeCas9 sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC025

   Plasmid

   #227597

   Purpose

   Lentiviral backbone used when cloning pooled prime editing guide RNA libraries already containing a tevopreQ1 RNA motif

   Depositor

   Brittany Adamson

   Article

   Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1(+) mFis1 V1

   Plasmid

   #44600

   Depositor

   David Chan

   Article

   Loson et al Mol Biol Cell. 2013 Jan 2.

   Insert

   mFis1 variant 1 (Fis1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAC026

    Plasmid

    #227598

    Purpose

    Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)

    Depositor

    Brittany Adamson

    Article

    Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. EC64422

    Plasmid

    #191768

    Purpose

    CRISPR mutagenesis in Medicago truncatula

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:221-252. doi

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-FRT-TO-N-Flag-8His

    Plasmid

    #157677

    Purpose

    N-terminal tagging vector

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Flag-8His
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pcDNA5-FRT-TO-eGFP

    Plasmid

    #157733

    Purpose

    mammalian expression of untagged eGFP under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(-) MID49 4xMyc Hisx6

    Plasmid

    #44596

    Depositor

    David Chan

    Article

    Loson et al Mol Biol Cell. 2013 Jan 2.

    Insert

    MiD49 (Mief2 Mouse)
    

    Use

    Tags

    4xMyc and 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(-) MID51 4xMyc Hisx6

    Plasmid

    #44598

    Depositor

    David Chan

    Article

    Loson et al Mol Biol Cell. 2013 Jan 2.

    Insert

    MiD51 (Mief1 Mouse)
    

    Use

    Tags

    4xMyc and 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. tol2-CAG::ChR2-YFP

    Plasmid

    #59740

    Purpose

    Optogenetic excitation of ES cell-derived neurons (and other cells, such as muscle)

    Depositor

    Ivo Lieberam

    Article

    Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

    Insert

    Channelrhodopsin-2
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    CAG

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

17. AAV-FLEX-VAMP2:HRP

    Plasmid

    #60659

    Purpose

    Labels synaptic vesicles for electron microscopy

    Depositor

    Scott Sternson

    Article

    Atasoy et al Nat Neurosci. 2014 Dec;17(12):1830-9. do

    Insert

    VAMP2:HRP
    

    Use

    AAV and Cre/Lox

    Tags

    Myc

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA5-FRT-TO-C-8His-Flag

    Plasmid

    #157678

    Purpose

    C-terminal tagging vector

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    8His-Flag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pcDNA3.1(+) mMff isoform 5

    Plasmid

    #44601

    Depositor

    David Chan

    Article

    Loson et al Mol Biol Cell. 2013 Jan 2.

    Insert

    mMff isoform 5 (Mff Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBS-PtH2B

    Plasmid

    #133390

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    No promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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