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J23100_PheS*_codon

Plasmid

#247070

Purpose

express codon optimized PheS* gene with constitutive promoter BBa_J23100

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

mutated (A294G) E.coli PheS of which codon is changed

Expression

Bacterial

Available Since

March 6, 2026

Availability

Academic Institutions and Nonprofits only

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pAT005

Plasmid

#247636

Purpose

to generate mRNA for membrane mStaygold(mBaoJin) for zebrafish

Depositor

Gokul Upadhyayula

Article

Tschanz et al MicroPubl Biol. 2025 Nov 25;2025:10.1791

Insert

2x Lyn Kinase membrane localization sequence

Use

Zebrafish mrna expression using sp6

Tags

mStayGold(BJ)

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pDQM139

Plasmid

#247638

Purpose

to generate mRNA for membrane mGold2t for zebrafish

Depositor

Gokul Upadhyayula

Article

Tschanz et al MicroPubl Biol. 2025 Nov 25;2025:10.1791

Insert

2x Lyn Kinase membrane localization sequence

Use

Zebrafish mrna expression using sp6

Tags

mGold2t

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pDQM138

Plasmid

#247637

Purpose

to generate mRNA for membrane mGold2s for zebrafish

Depositor

Gokul Upadhyayula

Article

Tschanz et al MicroPubl Biol. 2025 Nov 25;2025:10.1791

Insert

2x Lyn Kinase membrane localization sequence

Use

Zebrafish mrna expression using sp6

Tags

mGold2s

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pDQM049

Plasmid

#247635

Purpose

to generate mRNA for membrane mStayGold(E138D) for zebrafish

Depositor

Gokul Upadhyayula

Article

Tschanz et al MicroPubl Biol. 2025 Nov 25;2025:10.1791

Insert

2x Lyn Kinase membrane localization sequence

Use

Zebrafish mrna expression using sp6

Tags

mStayGold(E138D)

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pAT002

Plasmid

#248842

Purpose

to generate mRNA for membrane mNeonGreen for zebrafish

Depositor

Gokul Upadhyayula

Article

Tschanz et al MicroPubl Biol. 2025 Nov 25;2025:10.1791

Insert

2x Lyn Kinase membrane localization sequence

Use

Zebrafish mrna expression using sp6

Tags

mNeonGreen

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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EML8543_pEML41003

Plasmid

#237864

Purpose

A suicide vector for Agrobacterium with kanamycin resistance.

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Type

Empty backbone

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGRNA1_105nt

Plasmid

#247075

Purpose

L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets pheS*_codon

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

type I-E CRISPR cas crRNA

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMut3_pm32B

Plasmid

#247071

Purpose

L-arabinose inducible pmCDA-XTEN32-CasB(Cas11)-GSSG-UGI

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

PmCDA-XTEN32-Cas11(CasB)-UGI

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pMut12_apo64C

Plasmid

#247072

Purpose

L-arabinose inducible rAPOBEC-XTEN64-CasC(Cas7)-GSSG-UGI

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

rAPOBEC-XTEN64-CasC-UGI

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGRNA2I_LacZB1

Plasmid

#247076

Purpose

L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets Ecoli LacZ B1 site

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

type I-E CRISPR cas crRNA

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pOP576

Plasmid

#85943

Purpose

Temperature sensitive plasmid based on pDK46. Expresses dCas9 from proD. Serves as a control for pOP587 - pOP594

Depositor

Timothy Lu

Article

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

dCas9

Expression

Bacterial

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pOP549

Plasmid

#85941

Purpose

Bi-stable switch, similar to pOP512 but with a lambdaCI OR_1 operator in ptrc-2 promoter driving tetR.

Depositor

Timothy Lu

Article

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

Bi-stable switch

Expression

Bacterial

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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memGRKct-Venus

Plasmid

#234745

Purpose

Mammalian expression of plasma membrane anchored (GAP43) GRKct-Venus

Depositor

Nevin Lambert

Article

Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

Insert

memGRKct-Venus

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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mRuby2-MOA

Plasmid

#234747

Purpose

Mammalian expression of mitochondria marker mRuby2-MOA

Depositor

Nevin Lambert

Article

Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

Insert

mRuby2-MOA

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCF

Plasmid

#216326

Purpose

PUlaA promoter driving expression of sfgfp

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

Superfolder green fluorescent protein

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pUlaR

Plasmid

#216327

Purpose

T7 promoter driving expression of ulaR

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

L-Ascorbate-6-P-responsive transciptional repressor

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBad_UlaABC

Plasmid

#216328

Purpose

Arabinose-inducible PBad promoter driving expression of ulaA, ulaB, and ulaC

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

UlaABC

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSuccessor

Plasmid

#228391

Purpose

intended to be co-transformed along with pDawn-Ag43 to generate successor strain (SS-GFP) for multipattern biofilm lithography

Depositor

Ingmar Riedel-Kruse

Article

Jin et al Nat Commun. 2024 Nov 1;15(1):9443. doi:

Insert

pConst23100-sfGFP__pLambda-DHFR

Use

Synthetic Biology

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDifferentiation

Plasmid

#228393

Purpose

intended to be co-transformed along with pDawn-Ag43 to generate differentiation strain (DS-mRuby2) for multipattern biofilm lithography

Depositor

Ingmar Riedel-Kruse

Article

Jin et al Nat Commun. 2024 Nov 1;15(1):9443. doi:

Insert

pLambda-luxR+LVAtag__pLux-mRuby2

Use

Synthetic Biology

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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