We narrowed to 8,093 results for: gnal

1. Ypd1p-pET22b

   Plasmid

   #12655

   Depositor

   SeWon Suh

   Article

   Song et al J Mol Biol. 1999 Nov 5. 293(4):753-61.

   Insert

   YPD1 protein (YPD1 Budding Yeast)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pMT Draper-delta ITAM-GFP

   Plasmid

   #126655

   Purpose

   Inducible expression of Draper-delta ITAM-GFP mutant in S2 cells

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   Draper with ITAM Tyr residues mutated to Ala
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Cytoplasmic ITAM Tyr residues mutated to Phe

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMT FRB-Draper INT-GFP

   Plasmid

   #126665

   Purpose

   Inducible expression of a synthetic FRB-Draper receptor in S2 cells

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   FRB-Draper INT domain
   

   Use

   Tags

   Expression

   Insect

   Mutation

   K986N- see depositor comments below

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET His10-Draper delta ITAM

   Plasmid

   #126671

   Purpose

   Expression of His10-Draper delta ITAM mutant for purification from E. coli

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   Draper cytoplasmic ITAM residues mutated to Phe
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Cytoplasmic ITAM residues mutated to Phe

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GFP-ROCK1

   Plasmid

   #187296

   Purpose

   Express GFP ROCK1

   Depositor

   Alpha Yap

   Article

   Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

   Insert

   Rock1 (a.k.a. 1110055K06Rik, Rock-I) (Rock1 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ER-RpHLuorin2

   Plasmid

   #171717

   Purpose

   Luminal ER expression of ratiometric pHLuorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2 targeted to ER
   

   Use

   Tags

   KDEL ER-retention signal and Signal sequence of h…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hNFAT2-409:TALE:VP64:KRɸ

   Plasmid

   #160661

   Purpose

   Constitutive expression of a truncated version of hNFAT (aa 2-409) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.

   Depositor

   Roman Jerala

   Article

   Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

   Insert

   hNFAT2-409:TALE:VP64:KRɸ
   

   Use

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1(+)IRES GFP

   Plasmid

   #51406

   Purpose

   Expresses GFP from internal ribosome entry sequence, cloning component

   Depositor

   Kathleen_L Collins

   Article

   Schaefer et al J Immunol. 2008 Jun 15;180(12):7804-17.

   Insert

   IRES GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-MAPPER

   Plasmid

   #117721

   Purpose

   MAPPER Selectively Labels ER-PM Junctions

   Depositor

   Jen Liou

   Article

   Chang et al Cell Rep. 2013 Nov 14;5(3):813-25. doi:

   Insert

   MAPPER
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Halo-KDEL

    Plasmid

    #124316

    Purpose

    Expresses ER specific HaloTag protein

    Depositor

    Jin Wang

    Article

    Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

    Insert

    Halo tag
    

    Use

    Tags

    KDEL Seq

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. DYSF-3HA

    Plasmid

    #29767

    Depositor

    Steven Vogel

    Article

    Covian-Nares et al J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-

    Insert

    DYSF (DYSF Human)
    

    Use

    Tags

    3xHA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. hBACCS2

    Plasmid

    #67625

    Purpose

    Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    hBACCS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA3 Flag Jnk2a2

    Plasmid

    #13801

    Depositor

    Roger Davis

    Article

    Sluss et al Mol Cell Biol. 1994 Dec . 14(12):8376-84

    Insert

    Jnk2a2 (MAPK9 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

14. TagBFP2-CAAX

    Plasmid

    #116856

    Purpose

    plasma membrane marker

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    HRAS
    

    Use

    Tags

    TagBFP2

    Expression

    Mammalian

    Mutation

    Amino acids 172-189

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNDL-60

    Plasmid

    #161720

    Purpose

    In vitro transcription construct for production of cytosolic acvr2b-CIB1N mRNA

    Depositor

    Adam Cohen

    Article

    McNamara et al bioRxiv [Preprint]. 2024 Apr 12:2024.04.

    Insert

    cytosolic acvr2b-CIB1N (acvr2b Zebrafish)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGP-CMV-GCaMP6s-CAAX

    Plasmid

    #52228

    Purpose

    Plasma membrane targeted GCaMP6s, calcium sensor

    Depositor

    Tobias Meyer

    Article

    Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

    Insert

    GCaMP6s-CAAX
    

    Use

    Tags

    6xHis and CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

    Plasmid

    #60910

    Purpose

    dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    dSV40 Promoter

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5M-ADAM10

    Plasmid

    #31717

    Depositor

    Rik Derynck

    Article

    Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

    Insert

    ADAM10 (ADAM10 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLVX-puro_GCaMP6s

    Plasmid

    #164589

    Purpose

    Stable integration and expression of GCaMP6s in mammalian cells

    Depositor

    Joseph Hyser

    Article

    Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

    Insert

    GCaMP6s
    

    Use

    Lentiviral

    Tags

    6xHis, T7 tag, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

    Plasmid

    #60904

    Purpose

    The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge