We narrowed to 31,648 results for: REP
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Plasmid#197891PurposeCan be used to generate AAV virus that will express ChRWR/Venus in the presence of Cre and the leakage expression is significantly reduced without CreDepositorInsertChRWR/Venus
UseAAVMutationN/APromoterEF1aAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::UMSBP::sNluc2_ORF1
Plasmid#186765PurposeAn mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at the endogenous gene's start codon.DepositorInsertNanoluciferase
PromoterT7Available SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::ENO::sNluc2
Plasmid#186770PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) with enolase 5' and 3' UTRsDepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::sNluc0
Plasmid#186771PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0) with RPL15 5' and 3' UTRsDepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::hNluc
Plasmid#186772PurposeAn mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with RPL15 5' and 3' UTRsDepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::hNluc
Plasmid#186774PurposeAn mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with y-box binding protein 5' and 3' UTRsDepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGAPDH::sNluc1
Plasmid#187221PurposeA plasmid containing a planarian codon-optimized Nluc (sNluc1), driven by the planarian GAPDH promoter.DepositorInsertNanoluciferase
UseOverexpressionPromoterGAPDHAvailable SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc1
Plasmid#186754PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1, CAI = 0.925) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::ENO::sNluc1
Plasmid#186755PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with enolase 5' and 3' UTRs.DepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL10::sNluc1
Plasmid#186756PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL10 5' and 3' UTRs.DepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::sNluc1
Plasmid#186757PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL15 5' and 3' UTRs.DepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc0
Plasmid#186758PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0, CAI = 1.0) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc2
Plasmid#186759PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2, CAI =0.713) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc3
Plasmid#186760PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc3, CAI = 0.663) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc4
Plasmid#186761PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc4, CAI = 0.743) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc5
Plasmid#186762PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc5, CAI = 0.633) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::UMSBP::sNluc2_ORF2
Plasmid#186766PurposeAn mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at a start codon further downstream of the endogenous gene's start codon..DepositorInsertNanoluciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::sNluc2_STOP
Plasmid#186767PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) containing a premature stop codon.DepositorInsertNanoluciferase
MutationPremature stop codon at residue 16PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRps9-WT-IRES-GFP-HygR
Plasmid#190271PurposeExpress RPS9 WT mouse gene transcriptionally fused with eGFP. eGFP is translated from IRES.DepositorInsertribosomal protein RPS9
TagsIRES-eGFPExpressionMammalianPromoterCAGGS-CMVAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRps9-D95N-IRES-GFP-HygR
Plasmid#190272PurposeExpress RPS9 D95N-mutant mouse gene transcriptionally fused with eGFP. eGFP is translated from IRES.DepositorInsertribosomal protein RPS9
TagsIRES-eGFPExpressionMammalianMutationD95NPromoterCAGGS-CMVAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b MOSPD2 RD/LD [282-490]
Plasmid#186470PurposeExpression of human MOSPD2 (MSP domain from residue 282 to 490; mutant R404D/L406D, unable to bind FFAT motifs), fused to a 6HIS tag, in bacteriaDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
Tags6HISExpressionBacterialMutationMSP domain [282-490]Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry-SynaptoZip
Plasmid#177318PurposeAAV expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by human Synapsin promoterDepositorInsertmCherry-SynaptoZip (Vamp2 Rat)
UseAAVTagsMyc and mCherryExpressionMammalianPromoterhSynAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22gCfpUbiG4FER4P2A(#249)
Plasmid#184070Purposecyclofen-inducible GAL4 activation in zebrafish permanent transgenicDepositorInsertGal4bdFF-ERT2-P2A
UseZebrafish transgenesis (blue eyes)MutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Slc1a3-CreERT2 Targeting Vector
Plasmid#129409PurposeGene targeting vector to knock-in 2A-CreERT2 into the mouse Slc1a3 locus in place of a STOP codonDepositorInsertSlc1a3-2A-CreERT2 Targeting Vector (Slc1a3 Mouse)
UseCre/Lox and Mouse TargetingExpressionMammalianAvailable SinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
px335 Slc1a3 nickase 1
Plasmid#129410PurposeCas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 geneDepositorInsertsgRNA directing Cas9n to the proximity of Slc1a3 STOP codon (Slc1a3 Mouse)
UseCRISPR and Mouse TargetingExpressionMammalianAvailable SinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
px335 Slc1a3 nickase 2
Plasmid#129411PurposeCas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 geneDepositorAvailable SinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-RG-Linker-eGFP
Plasmid#176071PurposeEGFP fused to the C-terminus of a RG domain & a hygromycin resistance cassetteDepositorInsertRG
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1AAvailable SinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN1mcherry-NKD2EFMUT
Plasmid#174333Purposeencodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
TagsmCherry tagExpressionMammalianMutationNKD2132DFD134>AFA (substitutions of D132 and D…PromoterCMVAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4 3xFLAG Halo G-Linker nsp1
Plasmid#175426PurposeExpresses G-linker CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertG-linker nsp1
Tags3XFLAG HaloExpressionMammalianMutationG-linker in place of the RNase destabilization do…PromoterCMVAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4 3xFLAG Halo R119A K120A nsp1
Plasmid#175428PurposeExpresses R119A K120A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertnsp1 R119A K120A
Tags3XFLAG HaloExpressionMammalianMutationN-terminal mutants R119A K120APromoterCMVAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4 3xFLAG Halo E55A E57A K58A nsp1
Plasmid#175427PurposeExpresses E55A E57A K58A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertnsp1 E55A E57A K58A
Tags3XFLAG HaloExpressionMammalianMutationN-terminal mutants E55A E57A K58APromoterCMVAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4 3xFLAG Halo E36A E37A nsp1
Plasmid#175429PurposeExpresses E36A E37A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertnsp1 E36A E37A
Tags3XFLAG HaloExpressionMammalianMutationN-terminal mutants E36A E37APromoterCMVAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO-3XFLAG Halo R99A nsp1
Plasmid#175422PurposeExpresses R99A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertCoV2 R99A nsp1
Tags3XFLAG HaloExpressionMammalianPromoterCMVAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔEC gRNA resistant
Plasmid#163413PurposeExpresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
ExpressionMammalianMutationThe extracellular domain (27-150 aa) is deleted. …Available SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔTail gRNA resistant
Plasmid#163412PurposeExpresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
ExpressionMammalianMutationThe C-terminal tail domain (530-1038 aa) is delet…Available SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQlinkG2-PTPIP51(236-470)
Plasmid#170534PurposeExpresses Cleavable GST-tagged PTPIP51 TPR domain (236-470) in E. ColiDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
TagsGST tagExpressionBacterialPromoterT5 promoterAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFASTbac-HisGST-PTPIP51(36-470)
Plasmid#170531PurposeExpresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9DepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 )
TagsGST tag and His tagExpressionInsectPromoterPolyhedrinAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQlinkH-PTPIP51(236-470)
Plasmid#170530PurposeExpresses Cleavable His-tagged PTPIP51 TPR domain (236-470) in E. ColiDepositorInsertProtein tyrosine phosphatase interacting protein 51 (236-470) (RMDN3 Human)
TagsHis tagExpressionBacterialPromoterT5 promoterAvailable SinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
YF
Plasmid#171459PurposeExpresses YF, a FiCE of iCMM, in mammalian cellsDepositorInsertEYFP-FKBP12
ExpressionMammalianPromoterCMVAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
mYF
Plasmid#171460PurposeExpresses mYF, a CiCE of NiCMM, in mammalian cellsDepositorInsertEYFP(A207K)-FKBP12
ExpressionMammalianPromoterCMVAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
mActZ-FmCh
Plasmid#171465PurposeExpresses mActZ-FmCh, a FiCE of iCMM, in mammalian cellsDepositorInsertActA(30-262 aa)-Zyxin(2-380 aa)-FKBP12-mCherry
ExpressionMammalianPromoterCMVAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42 -SSRP1
Plasmid#170439PurposeExpress drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.DepositorAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUMO ACD11
Plasmid#170738PurposeThe plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.DepositorAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
PWPXLD-EGFR-P667A
Plasmid#133750PurposeEGFR with mutation at proline 667 converted to alanine and tagged with HA cloned into PWPXLD plasmidDepositorInsertMutant P667A EGFR-HA (EGFR Human)
UseLentiviralTagsHA tagExpressionMammalianMutationproline 667 converted to alanine (Please see depo…PromoterEF1-alphaAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_∆R13–DD-talin1 (1-2299)
Plasmid#166125PurposeFor expression of mouse talin-1 (residues 1-2299) with C-terminal deletion of dimerization domain and R13 (∆R13-DD) in mammalian cells. Contains C-terminal EGFP tag.DepositorInsert∆R13–DD-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationresidues 1-2299, deletion of dimerization domain …Available SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_∆DD-talin1 (1-2493)
Plasmid#166124PurposeFor expression of mouse talin-1 (residues 1-2493) containing C-terminal deletion of dimerization domain (∆DD) in mammalian cells. Contains C-terminal EGFP tag.DepositorInsert∆DD-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationresidues 1-2493, deletion of dimerization domain …Available SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGxxFP-CD55
Plasmid#153959PurposeUsed for EGxxFP assay with CD55 intron 3, exon 4, and intron 4 sequencesDepositorInsertCD55 (a 1,016-bp fragment from intron 3, exon 4, and intron 4)
UseA plasmid specific for egxxfp assayTagsN/AExpressionMammalianMutationNo mutationPromoterCAG promoterAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
prCOrev
Plasmid#153961PurposeUsed to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)DepositorInsert5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the opposite direction
UseRetroviral; A reporter plasmid detecting dna reco…TagsNLS-ZFExpressionMammalianMutationNo mutationPromoterLTRAvailable SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
prCO
Plasmid#153960PurposeUsed to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)DepositorInsert5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the same direction
UseRetroviral; A reporter plasmid detecting dna reco…TagsNLS-ZFExpressionMammalianMutationNo mutationPromoterLTRAvailable SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA BRCA2 R2896C
Plasmid#139511PurposePlasmid expressing a sgRNA to introduce BRCA2 R2896C using base editingDepositorInsertsgRNA to insert BRCA2 E2896C using base editing
ExpressionMammalianPromoterU6Available SinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only