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We narrowed to 11,344 results for: cat.1

Showing: 5351 - 5400 of 11344 results
  1. pCMV-MTS-FLAG-LOV*

    Plasmid
    #202207
    Purpose
    Expresses the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cells
    Depositor
    Insert
    Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, aa 1-24
    Tags
    FLAG and LOV*
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pLenti-EF1a-MTS-FLAG-LOV*

    Plasmid
    #202208
    Purpose
    Plasmid for the generation of a lentiviral vector encoding the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cells
    Depositor
    Insert
    Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, aa 1-24
    Use
    Lentiviral
    Tags
    FLAG and LOV*
    Promoter
    Ef1a
    Available Since
    Aug. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pIBA2-SpyTag-MBP

    Plasmid
    #107422
    Purpose
    Control construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.
    Depositor
    Insert
    Maltose-binding protein (MalE)
    Tags
    OmpA signal peptide, SpyTag, and StrepII
    Expression
    Bacterial
    Promoter
    Tet
    Available Since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pATB_346

    Plasmid
    #226091
    Purpose
    Plasmid encodes an AT-FP with OprF-spacer signal peptide, ∆epitope∆β1∆connecting region linker, EhaA translocation unit enabling the display of a passenger of interest at the bacterial cell surface
    Depositor
    Insert
    default passenger (=insert) is cel5
    Tags
    His
    Expression
    Bacterial
    Promoter
    Cell-density inducible (Prox306)
    Available Since
    May 22, 2026
    Availability
    Academic Institutions and Nonprofits only
  5. pATB_336

    Plasmid
    #226085
    Purpose
    Plasmid encodes an AT-FP with CtxB-spacer signal peptide, ∆epitope∆β1∆connecting region linker, EhaA translocation unit enabling the display of a passenger of interest at the bacterial cell surface
    Depositor
    Insert
    default passenger (=insert) is cel5
    Tags
    His
    Expression
    Bacterial
    Promoter
    Cell-density inducible (Prox306)
    Available Since
    May 22, 2026
    Availability
    Academic Institutions and Nonprofits only
  6. GB1531

    Plasmid
    #215242
    Purpose
    TU for constitutive expression of Streptomyces phage phiC31 integrase. Catalyzes site-specific recombination between phiC31 attB and attP sites. Contains SV40 NLS. N. benthamiana codon optimized.
    Depositor
    Insert
    PNos:PhiC31:TNos
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB3

    Plasmid
    #201534
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Insert
    ilvA-relE_RBS3_CB3
    Use
    Synthetic Biology
    Mutation
    rhaS W190G
    Promoter
    PrhaBAD (rhamnose)
    Available Since
    Sept. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pEM047 [T7-assisted CROPseq sgRNA expression vector]

    Plasmid
    #224899
    Purpose
    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA
    Depositor
    Inserts
    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pTDpelB-NTwinStrep

    Plasmid
    #45940
    Purpose
    use to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequence
    Depositor
    Type
    Empty backbone
    Tags
    6xHis, PelB, and Twin-Strep
    Expression
    Bacterial
    Promoter
    Trc (lactose/IPTG inducible)
    Available Since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  10. pET30a-SSDH

    Plasmid
    #239573
    Purpose
    Expresses E. coli succinate semialdehyde dehydrogenase (SSDH/gabD) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.
    Depositor
    Insert
    Succinate-semialdehyde dehydrogenase (gabD E. coli)
    Tags
    His6
    Expression
    Bacterial
    Available Since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. pTDpelB-C_sfYFPTwinStrep

    Plasmid
    #45944
    Purpose
    expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequence
    Depositor
    Insert
    synthetic sfYFP (codon usage adapted to P.putida KT2440)
    Tags
    PelB export sequence and Twin-Strep-tag
    Expression
    Bacterial
    Promoter
    lacIq/Ptrc
    Available Since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  12. pTon1(acgfp1)

    Plasmid
    #233648
    Purpose
    Expression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.
    Depositor
    Insert
    target sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
    Use
    Amphibian expression, tet-on
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
  13. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2

    Plasmid
    #201533
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Insert
    ilvA-relE_RBS3_CB2
    Use
    Synthetic Biology
    Mutation
    rhaR L128+1bp (frameshift), ilvA G455C
    Promoter
    PrhaBAD (rhamnose)
    Available Since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
  14. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4

    Plasmid
    #201535
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Insert
    ilvA-relE_RBS3_CB4
    Use
    Synthetic Biology
    Mutation
    rhaS ΔM1-H5, PrhaSR Δ179bp
    Promoter
    PrhaBAD (rhamnose)
    Available Since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
  15. pCMV-TALEN-Rep

    Plasmid
    #45964
    Depositor
    Insert
    beta-Galactosidase
    Expression
    Mammalian
    Mutation
    duplicated aa 1-135
    Promoter
    CMV
    Available Since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
  16. pCMV-Rab-Rep

    Plasmid
    #45967
    Depositor
    Insert
    beta-Galactosidase
    Expression
    Mammalian
    Mutation
    duplicated aa 1-135
    Promoter
    CMV
    Available Since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 5351 - 5400 of 11344 results