9,092 results
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pcDNA3.1 PRDM16
Plasmid#15503DepositorInsertPRDM16 (Prdm16 Mouse)
ExpressionMammalianAvailable SinceDec. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH Yap S127A
Plasmid#86497PurposeMammalian expression of FLAG-tagged Yap containing S127A mutationDepositorInsertYes associated protein 1 (YAP1 Human)
TagsFLAGExpressionMammalianMutationS127APromoterEF1aAvailable SinceFeb. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLPCX-VEcadTL
Plasmid#45849DepositorAvailable SinceSept. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-HK2
Plasmid#23854DepositorInsertHK2 (HK2 Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXA1
Plasmid#153109PurposeExpression of Flag tagged human FOXA1 in mammalian cellsDepositorAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SARS-CoV-2 N
Plasmid#158079PurposeExpresses SARS-CoV-2 nucleocapsid protein in mammalian cellsDepositorInsertSARS-CoV-2 nucleocapsid (N )
ExpressionMammalianAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
YFP-tagged FRB (YR)
Plasmid#20148DepositorInsertYFP-FRB (YR)
ExpressionMammalianAvailable SinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-ER WT
Plasmid#49498PurposeExpresses HA-tagged ER wild-type in mammalian cellsDepositorAvailable SinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-GFP-LC3-RFP
Plasmid#84573PurposeExpresses GFP-LC3-RFP in mammalian cells to measure autophagic fluxDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseRetroviralTagsEGFP and mRFP1ExpressionMammalianAvailable SinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-NbALFA
Plasmid#159986PurposeFor the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmEGFP-NbALFA
Tags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mEGFP and NbALFAAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
DNMT3A-dCas9
Plasmid#100090PurposeDNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertDNMT3A (DNMT3A Human)
UseCRISPRTags3XFLag-NLS-DNMT3A-dCas9-NLSExpressionMammalianMutationincludes aa 602-912PromoterCMVAvailable SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR
Plasmid#42875PurposeA crRNA expression plasmid for targeting a specific sequence.DepositorInsertCRISPR-BsaI
UseCRISPR; E.coliExpressionBacterialAvailable SinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMJ920
Plasmid#42234DepositorInsertCas9
UseCRISPRTagsGFP, HA epitope, and NLS (nuclear localization si…ExpressionMammalianMutationcodon-optimized synthetic DNA sequencePromoterCMVAvailable SinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX330 spCas9-mSA
Plasmid#113096PurposepX330 vector carrying spCas9-mSA for gene editing in mammalian cell linesDepositorInsertCAS9-MSA
UseSynthetic BiologyTagsMSA (monomeric streptavidinMutationnoneAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-SAM
Plasmid#102559PurposePiggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.DepositorInsertsMS2-P65-HSF1_T2A_Hygro
dCAS9(D10A, N863A)-VP64_T2A_Blast
UseCRISPR; Piggybac transposonExpressionMammalianMutationD10A and N863A in Cas9 and N55K in MS2PromoterCAG and EF1AAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas9-sgAAVS1-2
Plasmid#129727PurposeExpressing SpCas9 and sgAAVS1-2DepositorInsertSpCas9 and sgAAVS1-2
UseCRISPRTagsT2A-mCherry-P2A-PuroExpressionMammalianPromoterhU6Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-Rpl22-HA
Plasmid#111811PurposeExpress Rpl22HA in astrocytesDepositorInsertRibosomal protein L22 (Rpl22 Mouse)
TagsHAAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Talin-CS
Plasmid#83377PurposeControl FRET probe for measuring tension on Talin moleculeDepositorInsertTalin1 (Tln1 Mouse)
UseRetroviralTagsEGFP-F40-tagRFP (tension sensor module)ExpressionMammalianMutationTension sensor module is inserted at C-terminal o…PromoterCMVAvailable SinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Mm.cargo(Cas9)
Plasmid#174865PurposeExpresses mouse Peg10 cargoRNA encoding SpCas9DepositorInsertSpCas9 flanked by MmPeg10 UTRs
TagsHA-SV40 NLS and SV40 NLSExpressionMammalianPromoterCMVAvailable SinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only
