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We narrowed to 6,530 results for: nls

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Showing: 4321 - 4360 of 6530 results

  1. 3xFLAG-dCas9/MSCV-EGFP

    Plasmid
    #82613
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g
    Insert
    3xFLAG-dCas9
    Use
    Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    82613-plasmid-map-sequence-id-298740

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  5. pYPQAT46

    Plasmid
    #223418
    Purpose
    T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for monocot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate ZmUbi1; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-Mb2Cas12a-RVRR-ZmUbi-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223418-plasmid-map-sequence-id-454166

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  18. pDIV300

    Plasmid
    #204968
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance marker
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    hph (hygromycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available Since
    Nov. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    204968-plasmid-map-sequence-id-454124

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  20. pCSN061

    Plasmid
    #101725
    Purpose
    Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.
    Depositor
    Rene Verwaal
    Article
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Inserts
    S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
    KanMX marker expression cassette.
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Promoter
    Heterologous TEF1 promoter from A. gossypii. and …
    Available Since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    101725-plasmid-map-sequence-id-199888

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  21. pTX209

    Plasmid
    #89269
    Purpose
    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01 and OsPDS-gRNA02
    Depositor
    Daniel Voytas
    Article
    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:
    Insert
    OsPDS-gRNA01 and OsPDS-gRNA02
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Plant
    Promoter
    Maize Ubiquitin1 promoter
    Available Since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    89269-plasmid-map-sequence-id-177895

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  22. 3xFLAG-dCas9/pMSCVneo

    Plasmid
    #134982
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134982-plasmid-map-sequence-id-430627

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  23. pCMV MN (eGFP)

    Plasmid
    #89932
    Purpose
    Expresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.
    Depositor
    Carl Novina
    Article
    Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.
    Insert
    MN
    Tags
    HA
    Expression
    Mammalian
    Mutation
    M.SssI residues 2-272 fragment
    Promoter
    CMV
    Available Since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    89932-plasmid-map-sequence-id-177997

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  24. pCT

    Plasmid
    #60620
    Purpose
    Plasmid encoding iCas9 and tracrRNA on pRS415 backbone
    Depositor
    Huimin Zhao
    Article
    Bao et al ACS Synth Biol. 2014 Sep 19.
    Inserts
    iCas9
    tracrRNA
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Yeast
    Mutation
    changed Aspartate 147 to Tyrosine, Proline 411 to…
    Promoter
    RPR1p and TEF1p
    Available Since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    60620-plasmid-map-sequence-id-99574

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  36. pTX208

    Plasmid
    #89268
    Purpose
    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01 and OsYSA-gRNA02
    Depositor
    Daniel Voytas
    Article
    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:
    Insert
    OsYSA-gRNA01 and OsYSA-gRNA02
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Plant
    Promoter
    Maize Ubiquitin1 promoter
    Available Since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    89268-plasmid-map-sequence-id-177893

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Showing: 4321 - 4360 of 6530 results