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We narrowed to 5,579 results for: dre

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Showing: 5401 - 5450 of 5579 results

  2. pcDNA.3.1.ADRB2-NP

    Plasmid
    #134376
    Purpose
    Nanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    ADRB2-NP (ADRB2 Human)
    Tags
    Flag, natural peptide of nanoluciferase, and sign…
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    Aug. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134376-plasmid-map-sequence-id-306396

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  3. 3` CC: Hygro – H2B-GFP-N – loxP – mScarlet

    Plasmid
    #219565
    Purpose
    3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome
    Depositor
    Andrea Ventura
    Article
    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586
    Insert
    HygroR_2A_H2B-GFP-N_loxP_mScarlet
    Use
    Unspecified
    Promoter
    EF-1a
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    219565-plasmid-map-sequence-id-438331

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  5. 3` CC: Hygro – GFP-N – loxP – mScarlet

    Plasmid
    #219564
    Purpose
    3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosome
    Depositor
    Andrea Ventura
    Article
    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586
    Insert
    3` CC: Hygro – GFP-N – loxP – mScarlet
    Use
    Unspecified
    Promoter
    EF1-a
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    219564-plasmid-map-sequence-id-438335

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  7. pGEX6P1‐hsRNASEH2BCA

    Plasmid
    #108692
    Purpose
    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme
    Depositor
    Andrew Jackson, Martin Reijns
    Article
    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9
    Inserts
    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A (RNASEH2A Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Shine Dalgarno sequence upstream of start codon a…
    Promoter
    tac
    Available Since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    108692-plasmid-map-sequence-id-208976

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  9. pGEX6P1‐hsRNASEH2BCA(D34A/D169A)

    Plasmid
    #108693
    Purpose
    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzyme
    Depositor
    Andrew Jackson, Martin Reijns
    Article
    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9
    Inserts
    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A-D34A/D169A
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Shine Dalgarno sequence upstream of start codon, …
    Promoter
    tac
    Available Since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    108693-plasmid-map-sequence-id-208987

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  13. pAAV-EF1a-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

    Plasmid
    #220612
    Purpose
    Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-KORD-P2A-ArgiNLS-AausFP1
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    220612-plasmid-map-sequence-id-441117

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  16. pFastBac-GFP-dynein-2(D1091–Q4307)

    Plasmid
    #64064
    Purpose
    Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.
    Depositor
    Andrew Carter
    Article
    Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi
    Insert
    Cytoplasmic dynein-2 (DYNC2H1 Human)
    Tags
    GFP, His tag, and ZZ tag
    Expression
    Insect
    Mutation
    Deleted amino acids 1-1090, extra C-terminal vali…
    Promoter
    Polyhedrin
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    64064-plasmid-map-sequence-id-110915

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  18. pAAV-hSyn-DIO {hCAR}off-{hM4Di-mCherry}on-W3SL

    Plasmid
    #111397
    Purpose
    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON hM4Di-mCherry (Gi-coupled DREADD for neuronal silencing), W3SL cassette (for maximize cloning capacity)
    Depositor
    Adam Kepecs
    Article
    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi
    Inserts
    hCAR (CXADR Human)
    hM4Di-mCherry
    Use
    AAV
    Tags
    Myc and mCherry
    Expression
    Mammalian
    Promoter
    hSynapsin
    Available Since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    111397-plasmid-map-sequence-id-212158

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  19. pAAV-EF1a-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

    Plasmid
    #220613
    Purpose
    Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    220613-plasmid-map-sequence-id-441115

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  20. pAAV-EF1a-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

    Plasmid
    #220614
    Purpose
    Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
    Use
    AAV and Cre/Lox
    Tags
    3x HA; ArgiNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    220614-plasmid-map-sequence-id-441114

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  24. cryS96

    Plasmid
    #67519
    Purpose
    αB-crystallin minipeptide fused to ELP (S96).
    Depositor
    Andrew MacKay
    Article
    Wang et al J Control Release. 2014 Oct 10;191:4-14.
    Insert
    mini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Synthetic, Human)
    Tags
    elastin-like polypeptide (ELP) S96
    Expression
    Bacterial
    Mutation
    mini-αB-crystallin contains residues 73–92 of aB-…
    Promoter
    T7
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only

  27. pAAV-hSyn-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

    Plasmid
    #220608
    Purpose
    Neuron-specific, Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
    Use
    AAV and Cre/Lox
    Tags
    3x HA; ArgiNLS
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220608-plasmid-map-sequence-id-441203

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Showing: 5401 - 5450 of 5579 results