We narrowed to 22,775 results for: crispr

1. pSQT1313

   Plasmid

   #53370

   Purpose

   multiplex gRNA expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   None
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYL-kasOp-FnCas12a3

   Plasmid

   #177875

   Purpose

   Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

   Depositor

   Yunzi Luo

   Article

   Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

   Insert

   Streptomyces codon optimized the FnCas12a mutant EP16
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   kasOp*

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. ampl43

   Plasmid

   #161830

   Purpose

   pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

   Depositor

   Saeed Tavazoie

   Article

   Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCpf1b-sp

   Plasmid

   #122188

   Purpose

   Bacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection marker

   Depositor

   Cheng-Cai Zhang

   Article

   Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBECAb-apr

   Plasmid

   #122001

   Purpose

   A cytidine deaminase-mediated base-editing plasmid in Acinetobacter baumannii

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFC332

   Plasmid

   #87845

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   hph (hygromycin resistance marker)
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC1802_pmax-RBFOX1N-dCasRx-C

   Plasmid

   #118635

   Purpose

   Transient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoter

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   RBFOX1N-dCasRx-C
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330GFP-hU6-gRNA-hSpCas9

   Plasmid

   #128385

   Purpose

   GFP positive gRNA/hSpCas9 construct

   Depositor

   Budd A. Tucker

   Article

   Giacalone et al Curr Protoc Stem Cell Biol. 2018 Feb 28;

   Insert

   GFP, gRNA and hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFC333

   Plasmid

   #87844

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   ble (bleomycin resistance marker)
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-TO-nmdCas9-3XGFP

    Plasmid

    #64109

    Purpose

    Expression of Nm dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Nm dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. gRNA Cloning Vector Bbs I ver. 2

    Plasmid

    #85586

    Purpose

    An empty sgRNA expression vector. sgRNA sequence can be cloned into its Bbs I site. Its gRNA scaffold has efficient ver. 2 structure.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Sci Rep. 2016 Jul 28;6:30485. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-EFS-MCP-HALOnls

    Plasmid

    #121937

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    MCP-HALOnls
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9 (PX165)

    Plasmid

    #48137

    Purpose

    Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGG>nls-hCas9-nls-GFP

    Plasmid

    #99141

    Purpose

    Cas9-GFP fusion protein under the regulation of chicken beta-actin promoter

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

    Insert

    Cas9-GFP
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. sgOpti

    Plasmid

    #85681

    Purpose

    Lentiviral vector to express sgRNAs. The cloning site is BsmBI.

    Depositor

    David Sabatini, Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRS-XylS-dCas9

    Plasmid

    #220196

    Purpose

    mini-Tn7 dCas9 expression vector - XylS/pM promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    XylS/pM

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. p415-GalL-Cas9-CYC1t

    Plasmid

    #43804

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GalL

    Available since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-sgRNA(MS2)

    Plasmid

    #102560

    Purpose

    Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-TO-nmdCas9-3XmCherry

    Plasmid

    #64110

    Purpose

    Expression of Nm dCas9-3XmCherry in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Nm dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLH-spsgRNA2

    Plasmid

    #64114

    Purpose

    Vector for expression of Sp sgRNA2 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp sgRNA2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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