We narrowed to 30,665 results for: ica;

1. pNOC_hfnCas12a_crRNA NR2

   Plasmid

   #176248

   Purpose

   pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR and Synthetic Biology; Expression in micro…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNOC_hfnCas12a_crRNA NR1

   Plasmid

   #176247

   Purpose

   pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR and Synthetic Biology; Expression in micro…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNOC_hfnCas12a-Nlux_crRNA NR3

   Plasmid

   #176246

   Purpose

   pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNOC_hfnCas12a_crRNA NR3

   Plasmid

   #176249

   Purpose

   pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR and Synthetic Biology; Expression in micro…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. hsa-cdk2 3'UTR sicheck2 mut

   Plasmid

   #121124

   Purpose

   luciferase assay

   Depositor

   Qing Deng

   Article

   Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

   Insert

   hsa-cdk2 3'UTR mutated 199 binding (CDK2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   miR-199 seed binding site mutated

   Promoter

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tol2-lyzC-mcherry-2a-CDK2-D145N

   Plasmid

   #121132

   Purpose

   neutrophil specific expression

   Depositor

   Qing Deng

   Article

   Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

   Insert

   dre-D145N CDK2 DN (cdk2 Zebrafish)
   

   Use

   Tags

   mcherry-2a

   Expression

   Bacterial

   Mutation

   D145N point mutation DN

   Promoter

   neutrophil specific lyzC

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. dre-cdk2 3'UTR sicheck2 199 mut

   Plasmid

   #121122

   Purpose

   luciferase assay

   Depositor

   Qing Deng

   Article

   Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

   Insert

   dre-cdk2 3'UTR mutated 199 binding (cdk2 Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   miR-199 seed binding site mutated

   Promoter

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLPC-NMYC-mTRF1deltaBLM

   Plasmid

   #64163

   Purpose

   Retroviral vector expressing mouse TRF1 lacking BLM-binding motifs with N-terminal Myc tag

   Depositor

   Titia de Lange

   Article

   Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

   Insert

   mTRF1 (Terf1 Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Deleted amino acids 313-316 and 339-342 (both BLM…

   Promoter

   CMV

   Available since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1-CHRFAM7ADelta2bp-YFP

   Plasmid

   #62637

   Purpose

   mammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with YFP fused into M3-M4 loop

   Depositor

   Henry Lester

   Article

   Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

   Insert

   CHRFAM7A (CHRFAM7A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   there is a 2-bp deletion in exon 6 of CHRFAM7A an…

   Promoter

   CMV

   Available since

   March 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-DDX11-Flag-WT

    Plasmid

    #120727

    Purpose

    Expression of a Flag-tagged version of DDX11 WT in mammalian cells

    Depositor

    Francesca Pisani

    Article

    Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

    Insert

    DDX11 (DDX11 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-AAV-syn-jGCaMP8m-WPRE

    Plasmid

    #162375

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG PSAM4-5HT3 HC IRES EGFP

    Plasmid

    #119740

    Purpose

    Chemogenetic activator expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Insert

    PSAM4 a75HT3 HC (homo) IRES eGFP (CHRNA7, HTR3A Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F, R420Q, R424D, R428A

    Promoter

    CAG

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGP-AAV-syn-jGCaMP8s-WPRE

    Plasmid

    #162374

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONRp5-p2 SspB-mCherry-CShroom3

    Plasmid

    #170977

    Purpose

    C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.

    Depositor

    Miki Ebisuya

    Article

    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

    Insert

    CShroom3 (Shroom3 Mouse)
    

    Use

    Gateway cloning

    Tags

    SspB and mCherry

    Expression

    Mutation

    Isoform X2, deleted aminoacids 1 - 1388

    Promoter

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGP-AAV-syn-jGCaMP8f-WPRE

    Plasmid

    #162376

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RUSH reporter-HMGB1-SBP-GFP

    Plasmid

    #172357

    Purpose

    Monitoring of HMGB1 subcellular localization by fluorescence videomicroscopy

    Depositor

    Guido Kroemer

    Article

    Liu et al Sci Rep. 2017 Nov 2;7(1):14915. doi: 10.

    Insert

    high mobility group box 1 (HMGB1 Human)
    

    Use

    Lentiviral

    Tags

    SBP-GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV GFAP jGCaMP7b

    Plasmid

    #171118

    Purpose

    Astrocyte AAV-mediated expression of bright ultrasensitive protein calcium sensor under the GFAP promoter

    Depositor

    Thomas Oertner

    Article

    Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

    Insert

    jGCaMP7b
    

    Use

    AAV

    Tags

    6 His Tag, T7 Tag, and Xpress Tag

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-15b_LC3B

    Plasmid

    #73949

    Purpose

    For recombinant expression of human LC3B/MAP1LC3B in E. coli

    Depositor

    Dieter Willbold

    Article

    Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

    Insert

    LC3B (MAP1LC3B Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-hSyn-ASAP3-WPRE

    Plasmid

    #132331

    Purpose

    expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-N1_MeCP2(deltaNIC)

    Plasmid

    #110191

    Purpose

    Expression of mouse MeCP2 (deltaNIC) in mammalian cells

    Depositor

    Adrian Bird

    Article

    Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

    Insert

    MeCP2_e2 delta NIC (mouse cDNA) - EGFP (Mecp2 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta N and C termini. Intervening region replace…

    Promoter

    CMV

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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