3,614 results

1. pDmelOR-mRho.V5.mER.hOr47a

   Plasmid

   #126478

   Purpose

   Human codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells

   Depositor

   Bill Hansson

   Article

   Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

   Inserts

   Or47a (Or47a Fly)
   Orco (Orco Fly)
   

   Use

   Tags

   H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

   Expression

   Mammalian

   Mutation

   codon optimization for H. sapiens and codon optim…

   Promoter

   CMV

   Available since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT-Hrp48-ADARcd-E488Q-V5

   Plasmid

   #139685

   Purpose

   Expresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assays

   Depositor

   Michael Rosbash

   Article

   Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

   Insert

   Hrp48, ADAR (Adar Fly)
   

   Use

   Tags

   V5

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFD1-dU6:1gRNA

   Plasmid

   #49408

   Purpose

   expression of gRNA under control of the Drosophila U6:1 promoter

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   dU6-1:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6-1

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUASp YFP Rab5 WT

   Plasmid

   #53468

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab05 (Rab5 Fly)
   

   Use

   Tags

   YFP

   Expression

   Insect

   Mutation

   Promoter

   GAL4

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pCFD5-U6-3-t-attP40

   Plasmid

   #133561

   Purpose

   gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   attP40 region guide RNAs
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFJ910

   Plasmid

   #44481

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1, NeoR, MCS
   

   Use

   Multiple cloning site vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. K980

   Plasmid

   #129762

   Purpose

   Expresses Drosophila Kinesin-1 full-length for microtubule gliding assays

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUASp YFP Rabx6 WT

   Plasmid

   #53479

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rabx6 (RabX6 Fly)
   

   Use

   Tags

   YFP

   Expression

   Insect

   Mutation

   Promoter

   GAL4

   Available since

   May 12, 2014

   Availability

   Academic Institutions and Nonprofits only

9. doraWT_rescue_construct

   Plasmid

   #190609

   Purpose

   Puromycin-selectable expression of Dora (CG34401) in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Dorado (CG34401 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Actin-5c

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBac{3XP3::DsRed, Pactin::Ptrsps}

    Plasmid

    #86862

    Purpose

    Expresses DsRed in eyes and ubiquitous expression of P-transposase

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Insert

    pACNNTNPII-S129A
    

    Use

    Tags

    Expression

    Insect

    Mutation

    S129A

    Promoter

    Available since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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