We narrowed to 5,443 results for: 129

1. pNCST-AausFP2

   Plasmid

   #129500

   Purpose

   Expresses AausFP2 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AausFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MADR pDonor TagBFP2-3XFlag (cyto) WPRE

   Plasmid

   #129421

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of a TagBFP2-3XFlag cytoplasmic reporter protein

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   TagBFP2-3Flag
   

   Use

   Mosaic analysis for dual recombinase-mediated cas…

   Tags

   3X Flag epitope tag

   Expression

   Mutation

   Promoter

   none (3X polyA to mitigate episomal expression)

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNCST-AvicFP2

   Plasmid

   #129505

   Purpose

   Expresses AvicFP2 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AvicFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pExp-Calm

   Plasmid

   #129238

   Purpose

   Protein expression in E.coli

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   His and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKI-cjFOXP2-hum

   Plasmid

   #129757

   Purpose

   Gene targeting in marmoset cells

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

   Insert

   FOXP2 (LOC100411307 C. jacchus (common marmost))
   

   Use

   Marmoset targeting

   Tags

   Expression

   Mutation

   T301N/N323S

   Promoter

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pgRNA-guideless

   Plasmid

   #129464

   Purpose

   Derived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.

   Depositor

   Silvia Cardona

   Article

   Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

   Insert

   Guideless gRNA backbone
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119 (SpeI)

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-Asef2

   Plasmid

   #129610

   Purpose

   RhoGEF

   Depositor

   Dorus Gadella

   Article

   Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

   Insert

   Asef2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB-RTP1S-Integration

   Plasmid

   #129458

   Purpose

   piggyBac transposon vector that Inducibly (Tet-On) expresses RTP1S

   Depositor

   Sriram Kosuri

   Article

   Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

   Insert

   RTP1 (Rtp1 Mouse)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Removal of the first 36 amino acids of the protein

   Promoter

   TRE (Tet-On)

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-sgACSL3.C-Cas9-P2A-Puro

   Plasmid

   #129412

   Purpose

   Encoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminus

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   SpCas9 and sgACSL3.C (ACSL3 Human)
   

   Use

   CRISPR

   Tags

   P2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT2ADW_2paRAF

    Plasmid

    #129654

    Purpose

    For generating Tg mice expressing the 2P-activatable RAF system with P2A and full-length RAF1

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

    Insert

    2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. CytERM-mAvicFP1

    Plasmid

    #129510

    Purpose

    Expresses CytERM-mAvicFP1 in mammalian cells (for OSER assay)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    CytERM-mAvicFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. FUGAW

    Plasmid

    #129473

    Purpose

    3rd-generation lentiviral vector for hUbC-driven expression of GFP-aequorin

    Depositor

    Robert Gross

    Article

    Tung et al Neurophotonics. 2016 Apr;3(2):025001. do

    Insert

    GFP-aequorin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSL1433

    Plasmid

    #129523

    Purpose

    pDEF-SpCas9::GFP, Empty Vector for RGR and HDR Template, PyBiP-minimal promoter, 3rd Generation

    Depositor

    Scott Lindner

    Article

    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

    Insert

    SpCas9 (Other)
    

    Use

    Plasmodium, rodent-infectious species

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSC201

    Plasmid

    #129387

    Purpose

    A variant of the pSCrhaB2 vector with the oriR6K and mob genes from pGPΩ-TP. Used for homologous recombination of the rhamnose-inducible system into the chromosome of Burkholderia cenocepacia K56-2.

    Depositor

    Silvia Cardona

    Article

    Ortega et al J Bacteriol. 2007 May;189(9):3639-44. do

    Insert

    oriR6K and oriT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    N/A

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. NLS-QUE37C/pLBS CAG

    Plasmid

    #129322

    Purpose

    ATP measurement, nucleus, lentivirus

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN-37C
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCherry-FGD5

    Plasmid

    #129613

    Purpose

    RhoGEF

    Depositor

    Dorus Gadella

    Article

    Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

    Insert

    FGD5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-pEF1a-FLEX-HA-VHH-KASH-WPRE

    Plasmid

    #129704

    Purpose

    Express HA-KASH for nuclei labeling and purification

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    HA-VHH-KASH
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS hTSP-2

    Plasmid

    #12994

    Depositor

    Jack Lawler

    Insert

    TSP-2 (THBS2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pGL3-Basic-ACSL3-sfGFP(C) HDR template

    Plasmid

    #129413

    Purpose

    HDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFP

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    ACSL3 HDR template (ACSL3 Human)
    

    Use

    CRISPR and TALEN; Endogenous tagging hdr template

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MADR pDonor TagBFP2-V5-HRAS G12VWPRE

    Plasmid

    #129420

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    TagBFP2-V5-HRAS G12V (HRAS Entacmaea quadricolor (TagBFP), Human)
    

    Use

    Mosaic analysis for dual recombinase-mediated cas…

    Tags

    TagBFP2-V5 tag

    Expression

    Mutation

    G12V

    Promoter

    none (3x polyA to mitigate episomal expression)

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pUC-STITCH

    Plasmid

    #129535

    Purpose

    STITCH (Synthetic Toplogical Insulator with TetO for CHromatin-engineering) with homology arms for MYC locus insertion.

    Depositor

    Hideyuki Okano

    Article

    Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

    Insert

    STITCH
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Clim-DC2-NMM-tdtomato

    Plasmid

    #129557

    Purpose

    pClimDC-NMM:tdT cell membrane marker

    Depositor

    Inaki Ruiz-Trillo

    Article

    Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

    Insert

    N-Myristoylation motif (NMM) fused to tdtomato
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pZCAN

    Plasmid

    #129115

    Purpose

    Z. rouxii centromeric vector containing ClonNAT resistance

    Depositor

    Paolo Giudici

    Article

    Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ORF31-2xStrep pCDNA4

    Plasmid

    #129744

    Purpose

    Expresses KSHV ORF31 with Cterm 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

    Insert

    ORF31 (ORF31 Kaposi's sarcoma-associated herpesvirus (HHV-8))
    

    Use

    Tags

    2xStrep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pRSET QUEEN7mu R92A

    Plasmid

    #129367

    Purpose

    mutant of QUEEN-7mu

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN7mu R92A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRSET QUEEN7mu R99A

    Plasmid

    #129370

    Purpose

    mutant of QUEEN-7mu

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN7mu R99A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRSET-QUEEN 7mu A81D

    Plasmid

    #129351

    Purpose

    mutant of QUEEN-7mu

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN 7mu A81D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRSET-QUEEN 7mu E83A

    Plasmid

    #129353

    Purpose

    mutant of QUEEN-7mu

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN 7mu E83A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pETM6_Pfrm_BsMdh_BmHps_BmPhi

    Plasmid

    #129109

    Purpose

    Expresses Bacillus subtilis Mdh and Bacillus methanolicus Hps & Phi under Pfrm promoter

    Depositor

    Eleftherios Terry Papoutsakis

    Article

    Rohlhill et al ACS Synth Biol. 2017 Aug 18;6(8):1584-15

    Insert

    BsMDH BmHPS BmPHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pN1-NLS-Q2m

    Plasmid

    #129319

    Purpose

    QUEEN ATP sensor for 25degC, nucleus

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

    Insert

    QUEEN-2m
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge