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Showing: 301 - 324 of 324 results

  10. mKate2-P2A-APEX2-GBP

    Plasmid
    #67650
    Purpose
    Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.
    Depositor
    Rob Parton
    Article
    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:
    Insert
    APEX2-GBP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67650-plasmid-map-sequence-id-116990

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  11. pLaG2-T7RNAPC

    Plasmid
    #216337
    Purpose
    Arabinose-inducible PBad promoter driving expression of the anti-eGFP nanobody LaG2 translationally fused to the C-terminal portion (residues 180-883) of T7RNAP
    Depositor
    Mark Styczynski
    Insert
    LaG2-T7RNAPC fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    g2658a, g3915a
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    216337-plasmid-map-sequence-id-456751

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  13. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

    Plasmid
    #162877
    Purpose
    Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM
    Depositor
    Frederic Meunier
    Article
    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p
    Insert
    pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
    Use
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Insert consists of AA1-170
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    162877-plasmid-map-sequence-id-321985

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  14. attB53_SNCB+_attB53

    Plasmid
    #183614
    Purpose
    RMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Inserts
    LaG17 GFP nanobody SynNotch tTA
    TRE-mCherry-SV40pA
    tagBFP
    Use
    Rmce
    Tags
    Myc and human CD8a signal sequence
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183614-plasmid-map-sequence-id-361603

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  15. attB53_SNCB-_attB53

    Plasmid
    #183615
    Purpose
    RMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Inserts
    LaG17 GFP nanobody SynNotch tTA
    TRE-mCherry-SV40pA
    tagBFP
    Use
    Rmce
    Tags
    Myc and human CD8a signal sequence
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    183615-plasmid-map-sequence-id-366916

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  20. pMT[Hygro]_GBP-3xFlag-DEST

    Plasmid
    #157813
    Purpose
    A gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selection
    Depositor
    Zoltan Lipinszki
    Article
    Torras-Llort et al Commun Biol. 2020 Aug 19;3(1):454. doi:
    Insert
    GFP-binding protein
    Use
    For drosophila cells
    Tags
    3xFlag
    Expression
    Insect
    Mutation
    Promoter
    metallothionein
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    157813-plasmid-map-sequence-id-311081

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Showing: 301 - 324 of 324 results