1,878 results

1. pJF6

   Plasmid

   #139040

   Purpose

   MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   gfp (includes STOP codon, PATC introns)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKS2301

   Plasmid

   #37834

   Depositor

   Gary Silverman

   Article

   Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

   Insert

   Pnhx-2cpl-1::YFP (cpl-1 Nematode)
   

   Use

   Tags

   YFP

   Expression

   Worm

   Mutation

   Promoter

   nhx-2

   Available Since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKS235

   Plasmid

   #191855

   Purpose

   Negative selection marker for MosSCI

   Depositor

   K Subramaniam

   Article

   Revathi et al MicroPubl Biol. 2022 Oct 20;2022:10.1791

   Insert

   peel-1 toxic protein for negative selection against extrachromosomal array
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   hsp-16.41

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJS145

   Plasmid

   #179159

   Purpose

   Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.

   Depositor

   Iva Greenwald

   Article

   Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

   Insert

   rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Flexon replacing 2nd intron of 2xNLS::GFP

   Promoter

   rps-27p

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHZ081

   Plasmid

   #27506

   Depositor

   Andrew Fire

   Article

   Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

   Insert

   pcol-10::lin-4::let-858_3'UTR (lin-4 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

   Plasmid

   #159813

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJH3449

   Plasmid

   #179576

   Purpose

   Pacr-5::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans B-MN/others and others expression of miniSOG-SL2 BFP

   Depositor

   Mei Zhen

   Article

   Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

   Insert

   tomm20::miniSOG (tomm-20 Nematode)
   

   Use

   Tags

   BFP

   Expression

   Worm

   Mutation

   Promoter

   Plgc-55B

   Available Since

   March 1, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pCM1.127

   Plasmid

   #21384

   Depositor

   Geraldine Seydoux

   Article

   Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

   Insert

   pie-1 promoter (short, no ATG) in pDONRP4P1R
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 19, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EFF-1::V5

   Plasmid

   #132961

   Purpose

   Expresses EFF-1 A full length protein in mammalian cells

   Depositor

   Benjamin Podbilewicz

   Article

   Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

   Insert

   EFF-1::V5
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAP2

    Plasmid

    #171208

    Purpose

    bacterial expression for purification of PGL-3, allowing cleavage for MBP tag using TEV protease

    Depositor

    Geraldine Seydoux

    Article

    Schmidt et al bioRxiv 2020.10.15.340570

    Insert

    pgl-3 (pgl-3 Nematode)
    

    Use

    Tags

    MBP::HIS::TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ChrII_ttTi5605_loxP_myo2_neoR

    Plasmid

    #194056

    Purpose

    Dual-selection cassette plasmid for knocking-in at the ChrII_ttTi5605 of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    ttTi5605
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    None

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159850

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJW2341

    Plasmid

    #186950

    Purpose

    30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker

    Depositor

    Jordan Ward

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. BSP605

    Plasmid

    #122250

    Purpose

    expression vector for mCerulean under EFT3 promoter

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    mCerulean
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    EFT3

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ChrV_oxTi365_loxP_myo2_neoR

    Plasmid

    #194057

    Purpose

    Dual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    oxTi365
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    None

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSR49

    Plasmid

    #69259

    Purpose

    phlh-8::tagBFP2

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    TagBFP2
    

    Use

    Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0, TagBFP2 contains 2 sing…

    Promoter

    hlh-8

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJA281

    Plasmid

    #22513

    Depositor

    Julie Ahringer

    Article

    Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

    Insert

    Pmex-5::mCherry (w introns w/o stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    March 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJH4542

    Plasmid

    #200779

    Purpose

    Ptwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptune

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    GCaMP6
    

    Use

    Tags

    mNeptune

    Expression

    Worm

    Mutation

    Promoter

    Ptwk-40

    Available Since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pCM1.53

    Plasmid

    #17250

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al WormBook. 2010 Feb 8:1-21. doi: 10.1895/

    Insert

    GFP-no stop in pDONR201
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBT3011

    Plasmid

    #122539

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a lysosomal localized gene (cup-5) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_CemOrange2::cup-5 (cup-5 Nematode, Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pnhx-2

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBT3003

    Plasmid

    #122537

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (pisy-1) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_CemOrange2::pisy-1 (pisy-1 Nematode, Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pnhx-2

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAA173

    Plasmid

    #21743

    Depositor

    David Sherwood

    Article

    Ziel et al Nat Cell Biol. 2009 Feb . 11(2):183-9.

    Insert

    pie-1>mCherry::PH domain (PLC-delta)
    

    Use

    Bacterial propagation

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAS1-gpa-2P::his-24::Cerulean

    Plasmid

    #124337

    Purpose

    Multicolor Worms for Neuronal ID

    Depositor

    Andrew Leifer

    Insert

    gpa-2, Cerulean (gpa-2 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 3xHA-BioID_pAS28

    Plasmid

    #118223

    Purpose

    expresses 3xHA-tagged BioID in C. elegans intestine

    Depositor

    Jessica Feldman

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    BioID (BirA mutant)
    

    Use

    Tags

    3x HA tag

    Expression

    Worm

    Mutation

    R118G

    Promoter

    ges-1p

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJA256

    Plasmid

    #21509

    Depositor

    Julie Ahringer

    Article

    Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

    Insert

    (Gly)5Ala::GFP(w introns)::tbb-2 3'UTR
    

    Use

    Multisite gateway

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMS158

    Plasmid

    #215679

    Purpose

    Empty repair plasmid for ChrIII split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    5'HA + MCS + lox2272 + rps-0p::5'ΔHygR
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    5' ∆HYGR encodes aa1-226

    Promoter

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCFJ355 - MCS(ttTi14024, X)

    Plasmid

    #34870

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    ttTi14024 targeting
    

    Use

    Worm targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]

    Plasmid

    #159799

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGC185

    Plasmid

    #19624

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Prpl-28<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<4xNLS-tdimer2(12)::let-858(3’)
    

    Use

    Flp/frt

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBT3014

    Plasmid

    #122540

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a late endosome localized gene (rab-7) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_CemOrange2::rab-7 (rab-7 Nematode, Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pnhx-2

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge