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Plasmid#159870PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVE-FH-IN
Plasmid#70071Purpose2nd gen lentiviral vector. Cre-removable and tet/dox controllable expression of FLAG-HA-tagged transgene in mammalian cellsDepositorTypeEmpty backboneUseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2309 - [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
Plasmid#159861PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2443 - [1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159867PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLPC-CHES1L
Plasmid#53743Purposelong isoform of human CHES1DepositorInsertCHES1 (FOXN3 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2453 - [1-2] ENTR - Fluor - ce-mCardinal(900bp PATCs, NLS, no_atg, no_stop)
Plasmid#159863PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-mCardinal(900bp PATCs, NLS, no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAEL007584-Cas9
Plasmid#100706PurposeThe promoter of AAEL007584 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL007584-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectMutationPromoterAAEL007584 and Opie2Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2306 - [1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
Plasmid#159848PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2307 - [1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
Plasmid#159856PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
mlp-hCHES1sh
Plasmid#51845PurposeshRNA against CHES1DepositorInsertshRNA against CHES1 (FOXN3 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWZL-rCHES1
Plasmid#51847Purposeencodes a CHES1 mRNA that is resistant to shRNA against CHES1DepositorInsertCHES1 (FOXN3 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
NFlhb-t2a-GFP
Plasmid#194357Purposeover expression vector for killifish lhb tagged with GFPDepositorInsertluteinizing hormone subunit beta
UseLentiviralTagst2a-GFPExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2308 - [1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
Plasmid#159852PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-PIM2mut3
Plasmid#53742Purposepromoter region of PIM2 lacking the 2 forkhead binding sitesDepositorInsertPIM2 (PIM2 Human)
UseLuciferase; Reporter vectorTagsExpressionMutationdeletion of 2 forkhead binding sites (bp# 395-497…PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV/D377Y-mPCSK9
Plasmid#58376PurposeExpresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosisDepositorInsertproprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
UseAAVTagsExpressionMammalianMutationD377YPromoterHCRApoE/hAATAvailable sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
Plasmid#159816PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1137
Plasmid#84827PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized GFP
UseTagsExpressionBacterial and WormMutationPromoterPeft-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1415
Plasmid#84828PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 intronsDepositorInsertC. elegans codon optimized GFP
UseTagsExpressionBacterial and WormMutationPromoterPeft-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1320
Plasmid#84829PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 intronsDepositorInsertC. elegans codon optimized GFP
UseTagsExpressionBacterial and WormMutationPromoterPeft-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV/D374Y-hPCSK9
Plasmid#58379PurposeExpresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosisDepositorInsertproprotein convertase subtilisin/kexin type 9 (PCSK9 Human)
UseAAVTagsExpressionMammalianMutationD374YPromoterHCRApoE/hAATAvailable sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
DDX41-GFP
Plasmid#175494PurposeTotal RNA was isolated from TF-1 cells and converted to cDNA using SuperScript III (Invitrogen). The full DDX41 coding sequence was cloned into pcDNA3.1 (Invitrogen) with an EGFP c-terminal insert.DepositorInsertDDX41 (DDX41 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Zscan4 promoter
Plasmid#69249PurposeLuciferase reporter for Dux4 activation of Zscan4 transcriptionDepositorInsertZscan4 (ZSCAN4 Human)
UseLuciferaseTagsExpressionMutationThe two single nucleotide mismatches found betwee…PromoterZscan4Available sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1955
Plasmid#84830PurposeMinimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized tdTomato
UseTagsExpressionBacterial and WormMutationPromoterPeft-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-EGFP_opt
Plasmid#106334PurposeGateway Entry vector for EGFP (codon optimized for efficient gene synthesis)DepositorInsertEGFP
UseGateway entry vectorTagsExpressionBacterial and MammalianMutationcodon optimized for gene synthesis without affect…PromoterAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-BAP1-D672G-GFP
Plasmid#78919PurposeMammalian expression of BAP1 with D672G and an EGFP fusionDepositorInsertBAP1 (BAP1 Human)
UseTagsEGFP and HAExpressionMammalianMutationD672GPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-LynG
Plasmid#206867PurposeExpression of LynG probe - LynAnchor-eGFPDepositorInsertLynG
UseRetroviralTagseGFPExpressionMammalianMutationContains Lyn amino acids 1-16PromoterAvailable sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-BAP1-F81V-GFP
Plasmid#78912PurposeMammalian expression of BAP1 with F81V and an EGFP fusionDepositorInsertBAP1 (BAP1 Human)
UseTagsEGFP and HAExpressionMammalianMutationF81VPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-BAP1-A95D-GFP
Plasmid#78914PurposeMammalian expression of BAP1 with A95D and an EGFP fusionDepositorInsertBAP1 (BAP1 Human)
UseTagsEGFP and HAExpressionMammalianMutationA95DPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-BAP1-H169Q-GFP
Plasmid#78917PurposeMammalian expression of BAP1 with H169Q and an EGFP fusionDepositorInsertBAP1 (BAP1 Human)
UseTagsEGFP and HAExpressionMammalianMutationH169QPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-BAP1-C91G-GFP
Plasmid#78916PurposeMammalian expression of BAP1 with C91G and an EGFP fusionDepositorInsertBAP1 (BAP1 Human)
UseTagsEGFP and HAExpressionMammalianMutationC91GPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-BAP1-S63C-GFP
Plasmid#78911PurposeMammalian expression of BAP1 with S63E and an EGFP fusionDepositorInsertBAP1 (BAP1 Human)
UseTagsEGFP and HAExpressionMammalianMutationS63CPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-CHES1L
Plasmid#51836Purposeencodes the long isoform of human CHES1 and a Flag tagDepositorInsertCHES1 (FOXN3 Human)
UseRetroviralTagsFlagExpressionMutationPromoterAvailable sinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA-BAP1-S172R-GFP
Plasmid#78918PurposeMammalian expression of BAP1 with S172R and an EGFP fusionDepositorInsertBAP1 (BAP1 Human)
UseTagsEGFP and HAExpressionMammalianMutationS172RPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-BAP1-C91W-GFP
Plasmid#78913PurposeMammalian expression of BAP1 with C91W and an EGFP fusionDepositorInsertBAP1 (BAP1 Human)
UseTagsEGFP and HAExpressionMammalianMutationC91WPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ889
Plasmid#84826PurposeMinimos transposon with Psmu-1:smu-1:GFP:smu-1 UTR and cbr-unc-119 selectionDepositorInsertsmu-1 (smu-1 Nematode)
UseTagsGFPExpressionBacterial and WormMutationPromoterPsmu-1Available sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-BAP1-N102S-GFP
Plasmid#78915PurposeMammalian expression of BAP1 with N102S and an EGFP fusionDepositorInsertBAP1 (BAP1 Human)
UseTagsEGFP and HAExpressionMammalianMutationN102SPromoterCMVAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
Control-Control-LRG-GFP
Plasmid#225873PurposeTwo copies of guide RNA targeting a control sequenceDepositorInsertControl
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
miR5 HsATP10B
Plasmid#204481Purposetransfer plasmid for lentiviral vector production with miR for Hs ATP10BDepositorInsertATP10B (ATP10B Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe-CHES1-ER
Plasmid#51846Purposefusion protein between CHES1 and the ligand binding domain of estrogen receptorDepositorInsertCHES1 (FOXN3 Human)
UseRetroviralTagsERExpressionMutationPromoterAvailable sinceAug. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-CHES1H164A
Plasmid#51844PurposeFlag-CHES1L mutant with ponctual mutation on histidine 164DepositorInsertCHES1 (FOXN3 Human)
UseRetroviralTagsFlagExpressionMutationPromoterAvailable sinceAug. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-CHES1L-dC
Plasmid#51839PurposeFlag-CHES1L mutant lacking the C-terminal and NLS domainsDepositorInsertCHES1 (FOXN3 Human)
UseRetroviralTagsFlagExpressionMutationcontains CHES1 amino acids 1-205PromoterAvailable sinceAug. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-CHES1L-dN
Plasmid#51843PurposeFlag-CHES1L mutant lacking the N-terminal domainDepositorInsertCHES1 (FOXN3 Human)
UseRetroviralTagsFlagExpressionMutationcontains CHES1 amino acids 110-490PromoterAvailable sinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-dCas9
Plasmid#112233PurposeLentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2DepositorInsertsdCas9
Porumycin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationChanged: Aspartic acid 10 to Glycine, Histidine 8…PromoterEFS-NSAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo
Plasmid#183411PurposepiggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.DepositorInsertTet-on 3G
UseCRISPR and Synthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterEF1AAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetON-GFP
Plasmid#115495PurposeLentiviral plasmid for tet-inducible expression of GFP (generated from plasmid #20321)DepositorInserteGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP13A3 D498N
Plasmid#195850PurposeExpresses D498N mutant Homo sapiens ATP13A3DepositorInsertATPase 13A3 D498N (ATP13A3 Human)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_CDC50A
Plasmid#203694Purposeexpresses CDC50ADepositorInsertTMEM30A (TMEM30A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP10B - EmGFP
Plasmid#204474Purposetransfer plasmid for lentiviral vector production expressing Hs ATP10B WT fused with EmGFPDepositorInsertsUseLentiviralTagsEmGFPExpressionMutationPromoterCMVAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP10B_E210A
Plasmid#204475Purposetransfer plasmid for lentiviral vector production expressing Hs ATP10B E210A mutantDepositorInsertATP10B (ATP10B Human)
UseLentiviralTagsExpressionMutationE210APromoterCMVAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP10B_R153X
Plasmid#204476Purposetransfer plasmid for lentiviral vector production expressing Hs ATP10B R153X mutantDepositorInsertATP10B (ATP10B Human)
UseLentiviralTagsExpressionMutationR153XPromoterCMVAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only