We narrowed to 11,283 results for: 110

1. pcDNA3-HA-eEF2K(S441A/S445A)

   Plasmid

   #110161

   Purpose

   Expression of human eEF2K (S441A/S445A) in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   Kruiswijk et al Sci Signal. 2012 Jun 5;5(227):ra40. doi:

   Insert

   eEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S441A/S445A

   Promoter

   CMV

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMEXC3H

   Plasmid

   #110080

   Purpose

   Analogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   3C cleavage site - His10 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-2X-P2A-Puro

   Plasmid

   #110840

   Purpose

   Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-2X
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A and 2 tandem NLS sequences in the XTEN linker

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p079 pPNT6

   Plasmid

   #11072

   Depositor

   Ramesh Shivdasani

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUltra-Exon1Q145-Myc-B

   Plasmid

   #110489

   Purpose

   Lentiviral vector to express mutant N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)

   Depositor

   Baoji Xu

   Article

   Xu et al PLoS One. 2017 May 11;12(5):e0177610. do

   Insert

   Homo sapiens huntingtin (HTT Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   partial long 3'UTR wild type huntingtin exon…

   Promoter

   UbC

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. GuideVector_BbsI

   Plasmid

   #110692

   Purpose

   For rapid cloning and expression of sgRNAs in mammalian cells. Contains copGFP for easy FACS sorting.

   Depositor

   Douglas Mahoney

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCC324

   Plasmid

   #110165

   Purpose

   miniMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)

   Depositor

   Iva Greenwald

   Article

   de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

   Insert

   ERK-KTR-mClover-T2A-mCherry-H2B
   

   Use

   Minimos transposon

   Tags

   mCherry and mClover

   Expression

   Worm

   Mutation

   R61K change made in ERK-KTR CDS

   Promoter

   lin-31 promoter/enhancer

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHDR-CDKN2A-Ex2-CMV-EGFP

   Plasmid

   #110734

   Purpose

   Recombination template for replacing CDKN2A Exon2 with CMV-EGFP

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   EGFP
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJM100 (pUC18T-miniTn7T-gm-araC-ParaBAD)

   Plasmid

   #110554

   Purpose

   miniTn7 delivery plasmid with araC-ParaBAD inducible promoter

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   araC-ParaBAD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   AraC-ParaBAD

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. ECFP-Rab5C-S35N

    Plasmid

    #110504

    Purpose

    Dominant Negative

    Depositor

    Bernardo Mainou, Terence Dermody

    Article

    Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

    Insert

    Rab5C-S35N (RAB5C Human)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    S35N

    Promoter

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJM251 (miniCTX1-araC-ParaBAD)

    Plasmid

    #110567

    Purpose

    Integration vector for Pseudomonas aeruginosa with araC-ParaBAD inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    araC-ParaBAD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araC-ParaBAD

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Designed VEGF-binding scFv G6des13

    Plasmid

    #110213

    Purpose

    Yeast Surface Display of G6des13

    Depositor

    Sarel Fleishman

    Article

    Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

    Insert

    G6des13
    

    Use

    Tags

    cmyc tag

    Expression

    Yeast

    Mutation

    L A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95F

    Promoter

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSP1594_2x_NLS

    Plasmid

    #110625

    Purpose

    Mammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9)

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    None
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. KLHL3 (KLHL3A-c003)

    Plasmid

    #110251

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KLHL3 (KLHL3 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 - lacO

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEN35-CDKN2A-Ex2-L

    Plasmid

    #110736

    Purpose

    Encodes Cas9 nickase and gRNA targeting CDKN2A Exon2

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    CDKN2A gRNA (CDKN2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. hCCR5-mTFP1

    Plasmid

    #110193

    Purpose

    Encodes for human CCR5 coding sequence tagged with the mTFP1 FP

    Depositor

    Sergi Padilla Parra

    Article

    Iliopoulou et al Nat Struct Mol Biol. 2018 Sep;25(9):814-

    Insert

    hCCR5 (CCR5 Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pStr-KDEL-IRES-ST-mCherry

    Plasmid

    #110727

    Purpose

    Expresses an Str-KDEL Hook for the RUSH system and separately Sialyltransferase-mCherry

    Depositor

    David Stephens

    Article

    McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

    Inserts

    Str-KDEL
    STase sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    Str Streptavidin binding peptide (SBP) and mCherr…

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV and IRES

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-P(Per2)-DIO-mCherry-Venus-NLS-D2

    Plasmid

    #110058

    Purpose

    Per2 transcription fluorescence reporter

    Depositor

    Eric Erquan Zhang

    Article

    Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

    Insert

    Per2 promoter and mCherry and Venus (Per2 Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    NLS-D2

    Expression

    Mammalian

    Mutation

    Promoter

    Per2

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. ins:GCaMP6s; cryaa:RFP

    Plasmid

    #110285

    Purpose

    A zebrafish insulin promoter driving the calcium indicator, GCaMP6s. Contains red eye marker, and is flanked with I-SceI sites.

    Depositor

    Nikolay Ninov

    Article

    Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

    Insert

    GCaMP6s
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    insulin

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-xFNLS-P2A-Puro

    Plasmid

    #110872

    Purpose

    Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xFNLS(3.7)
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

    Promoter

    EF1s

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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