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pwtCas9-bacteria

Plasmid

#44250

Purpose

aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

wild-type Cas9

Use

CRISPR

Expression

Bacterial

Promoter

pLtetO-1

Available Since

April 11, 2013

Availability

Academic Institutions and Nonprofits only

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pBV-Luc

Plasmid

#16539

Purpose

luciferase reporter plasmid with very low basal activity

Depositor

Bert Vogelstein

Article

He et al Cell. 1999 Oct 29. 99(3):335-45.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3 p53 WT

Plasmid

#69003

Purpose

mammalian expression of wild type human p53

Depositor

David Meek

Article

Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

Insert

p53 (TP53 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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Flag Snail WT

Plasmid

#16218

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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ES-FUCCI

Plasmid

#62451

Purpose

SMX_DEST vector with 3 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
minPGK::Cherry-hCdt1(30-120)-bGHpA
PGK::HygroR-bGpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Flag-METTL3

Plasmid

#53739

Purpose

For expression of METTL3 (methyltransferase-like 3) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Methyltransferase-like 3 (METTL3 Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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eGFP STAT1 WT

Plasmid

#12301

Depositor

Alan Perantoni

Article

Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

Insert

STAT1 (STAT1 Human)

Tags

eGFP

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.S

Plasmid

#103006

Purpose

non-standard AAV2 rep-AAV-PHP.S cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

Synthetic construct isolate AAV-PHP.S VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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7TFP

Plasmid

#24308

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

7xTcf-FFluc // SV40-PuroR

Use

Lentiviral

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ATR WT

Plasmid

#31611

Depositor

Aziz Sancar

Article

Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

Insert

ATR (ATR Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag FKHR

Plasmid

#13507

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

FKHR (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pCTcon2

Plasmid

#41843

Depositor

Dane Wittrup

Article

Chao et al Nat Protoc. 2006;1(2):755-68.

Insert

CD20

Tags

HA and c-myc

Expression

Yeast

Available Since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pLJC5-Tmem192-2xFlag

Plasmid

#102929

Purpose

Lentiviral expression of a lysosomal tag (Tmem192-Flag)

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

Tmem192 (TMEM192 Human)

Use

Lentiviral

Tags

Flag

Promoter

ubc

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-dCas9-p300-P2A-PuroR

Plasmid

#83889

Purpose

Lentiviral Sp dCas9-p300-P2A-PuroR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
pac from Streptomyces alboniger

Use

CRISPR and Lentiviral

Tags

Flag and nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EFS

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV5 BRGI-Flag

Plasmid

#19143

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

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7TGP

Plasmid

#24305

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

7xTcf-eGFP // SV40-PuroR

Use

Lentiviral

Mutation

EGFP is GFP with S65T and F64L mutations

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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Rictor_2 shRNA

Plasmid

#1854

Depositor

David Sabatini

Article

Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Insert

Rictor (RICTOR Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 7, 2006

Availability

Academic Institutions and Nonprofits only

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pDONR207 SARS-CoV-2 NSP3

Plasmid

#141257

Purpose

Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Has Service

Cloning Grade DNA

Insert

NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3β-FLAG-CBP-HA

Plasmid

#32908

Depositor

Tso-Pang Yao

Article

Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

Insert

CBP (Crebbp Mouse)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only

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CTV

Plasmid

#15912

Depositor

Klaus Rajewsky

Article

Thai et al Science. 2007 Apr 27. 316(5824):604-8.

Insert

CAG-STOP-eGFP-ROSA26TV

Use

Cre/Lox; /stop

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

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