We narrowed to 6,904 results for: crispr cas9 plasmids

1. pCDNA3.3 CAS9 IRES-Dsred

   Plasmid

   #129444

   Purpose

   expression of FnCas9 and DsRed

   Depositor

   Oskar Laur

   Insert

   FnCas9 IRES-Dsred
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR_Wheat_live_Cas9

   Plasmid

   #126880

   Purpose

   Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and BpiI sites for sgRNA modules.

   Depositor

   Sophien Kamoun

   Insert

   Wheat_live_Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas9

   Plasmid

   #42876

   Purpose

   Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide

   Depositor

   Luciano Marraffini

   Article

   Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   tracr/Cas9
   

   Use

   CRISPR; E.coli

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. p-dCas9-HDAC1-Hygro

   Plasmid

   #104409

   Purpose

   transient expression of dCas9-HDAC1 fusion protein

   Depositor

   Stephan Beck

   Insert

   HDAC1 (HDAC1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. p-dCas9-LSD1-Hygro

   Plasmid

   #104406

   Purpose

   transient expression of dCas9-LSD1 fusion protein

   Depositor

   Stephan Beck

   Insert

   LSD1 (KDM1A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   IRATp970A0364D (Source Bioscience) has a P405H ch…

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG1guide_RUBY_EggCas9

   Plasmid

   #225983

   Purpose

   To make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. The transgenics can be selected by red color appearance of plants

   Depositor

   Imran Siddiqi

   Insert

   Guide RNA against AtAGAMOUS1
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35s promoter to Drive RUBY and DD45p to drive Cas9

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti-dCas9-ZIM3-KRAB-BFP

   Plasmid

   #196712

   Purpose

   CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-KRAB(ZIM3)-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p-dCas9-dTET1-H1672Y-D1674A-Hygro

   Plasmid

   #104413

   Purpose

   transient expression of dCas9-dTET1 fusion protein

   Depositor

   Stephan Beck

   Insert

   dTET1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   H1672Y-D1674A

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. p-dCas9-JMJD2A-S288G-T289G-Hygro

   Plasmid

   #104418

   Purpose

   transient expression of dCas9-dJMJD2A fusion protein

   Depositor

   Stephan Beck

   Insert

   dJMJD2A
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   S288G-T289G

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMST665_BB3_L 23_gRNAempty_cas9_BbsI

    Plasmid

    #90278

    Purpose

    BB3_L 23_gRNAempty_cas9_BbsI; CRISPR plasmid with empty gRNA spacer to incorporate gRNA, Cas9

    Depositor

    Michael Sauer

    Article

    Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

    Insert

    Cas9
    

    Use

    A. niger

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEasyCas9

    Plasmid

    #184909

    Purpose

    Expresses the Streptococcus pyogenes Cas9 protein

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cas9-NAT

    Plasmid

    #64329

    Purpose

    Cas9 expression cassette carried by a yeast single-copy episome plasmid

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMCS-rybozyme-IRES-CAS9

    Plasmid

    #64668

    Purpose

    Plasmid encoding multiple cloning site, rybozyme and IRES-CAS9.

    Depositor

    Wataru Fujii

    Article

    Yoshioka et al Sci Rep. 2015 Dec 16;5:18341. doi: 10.10

    Inserts

    CAS9
    HDV ribozyme
    

    Use

    CRISPR

    Tags

    Flag, Internal ribosome entry site, and guide RNA…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9(BB)-2A-Puro (PX459)

    Plasmid

    #48139

    Purpose

    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

15. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

    Plasmid

    #167901

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR Ef1a-dCas9-BFP-KRAB

    Plasmid

    #217304

    Purpose

    lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter

    Depositor

    Luke Gilbert

    Insert

    dCas9-tagBFP-KRAB
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Ef1alpha

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTRE3G-VP64-dCas9-VP64-ZsG

    Plasmid

    #192668

    Purpose

    Dox-inducible expression of VP64-dCas9-VP64 with 2A ZsGreen1

    Depositor

    Jeffrey Miner

    Insert

    VP64-dCas9-VP64, ZsGreen1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    TRE3G

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIVTR(T7A)-Cas9n-RT-T2A-PuroR

    Plasmid

    #172296

    Purpose

    In vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editing

    Depositor

    Patrik Verstreken

    Insert

    Cas9n-RT-T2A-PuroR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    H840A (Cas9)

    Promoter

    T7 promoter A-initiating

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-U6-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #42230

    Purpose

    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Has Service

    Cloning Grade DNA

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cas9 sgRNA vector

    Plasmid

    #68463

    Purpose

    Cas9 sgRNA vector for cloning

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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