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pCDNA3.1-H-Luc_cyto

Plasmid

#113929

Purpose

Plasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 hSCN5A_deltaSIV

Plasmid

#145771

Purpose

expresses human Nav1.5 lacking the SIV motif in mammalian cells

Depositor

Hugues Abriel

Article

Jespersen et al Biochem Biophys Res Commun. 2006 Oct 6;3

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Use

Tags

none

Expression

Mammalian

Mutation

Stop codon at Serine 2013

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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GATA3-pCDNA3.1

Plasmid

#115271

Purpose

Expresses human GATA3 in mammalian cells

Depositor

Arne S. Schaefer

Insert

GATA3 (GATA3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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Pyronic/pcDNA3.1(-)

Plasmid

#51308

Purpose

Pyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.

Depositor

Luis Felipe Barros

Article

San Martin et al PLoS One. 2014 Jan 21;9(1):e85780. doi:

Insert

Pyronic

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mBeRFP

Plasmid

#175173

Purpose

large-stokes shift red fluorescent protein mBeRFP

Depositor

Bernardo Sabatini

Article

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

mBeRFP

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TYMS

Plasmid

#100545

Purpose

mammalian expression of thymidylate synthetase

Depositor

Yuh-Shan Jou

Article

Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

Insert

thymidylate synthetase (TYMS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MEF2C-HA

Plasmid

#32515

Depositor

Andrew Lassar

Article

Kozhemyakina et al Mol Cell Biol. 2009 Nov;29(21):5751-62.

Insert

Mef2c (Mef2c Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Orange_CaMBI_110

Plasmid

#124094

Purpose

orange calcium-modulated bioluminescent indicator, 110nM

Depositor

Michael Lin

Article

Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

Insert

Orange CaMBI 110

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Drp1 V2

Plasmid

#44599

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 variant 2 (Dnm1l Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NM23-H1

Plasmid

#10956

Depositor

Patricia Steeg

Article

Steeg et al J Natl Cancer Inst. 1988 Apr 6. 80(3):20

Insert

NM23 (NME1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-LSSmGFP

Plasmid

#186543

Purpose

LSSmGFP fluorescent protein (ex/em = 400/510 nm): cytosolic expression in mammalian cells

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Use

Tags

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-NEUROD1

Plasmid

#215031

Purpose

Mammalian expression of human NEUROD1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

neuronal differentiation 1 (NEUROD1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3'3xFlag

Plasmid

#208616

Purpose

Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag

Depositor

Quanfu Ma

Type

Empty backbone

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 acGFP

Plasmid

#128047

Purpose

Mammalian expression vector with N terminal AcGFP

Depositor

Oskar Laur

Type

Empty backbone

Use

Tags

AcGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ spike_del19

Plasmid

#155297

Purpose

Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles

Depositor

Raffaele De Francesco

Article

Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2

Insert

SARS-Cov2 spike_deleted (S SARS-Cov2)

Use

Generation of sars-cov-2-pseudotyped lentiviral p…

Tags

Expression

Mutation

Deletion in C-term 19 aa of spike

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1/FGFR2myc

Plasmid

#33248

Depositor

Alka Mansukhani, Claudio Basilico

Article

Mansukhani et al J Cell Biol. 2000 Jun 12. 149(6):1297-30

Insert

FGFR2 (Fgfr2 Mouse)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

KpnI site introduced at stop codon of FGFR2 to be…

Promoter

CMV

Available since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Dendra2

Plasmid

#103967

Purpose

Mammalian expression of fluorescent protein Dendra2 used for transfection efficiency control

Depositor

Qing Deng

Article

Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

Insert

Dendra2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-hMcl-1

Plasmid

#25375

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

MCL1 (MCL1 Human)

Use

Tags

V5 and His tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

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WT GBA pcDNA3.1

Plasmid

#188580

Purpose

Ectopic expression of wild-type GBA

Depositor

Anthony Schapira

Article

Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

Insert

GBA (GBA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Lifeact-mCherry

Plasmid

#67302

Purpose

Fluorescent indicator for F-actin

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

Lifeact-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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