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pBlastR-12xLexAOp-sfGFP-MiniWhite

Plasmid

#165916

Purpose

Blasticidin resistant 12xLexAOp sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable LexAOp Response Vector

Use

Synthetic Biology

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-5xQUAS-mCherry-MiniWhite

Plasmid

#165923

Purpose

Blasticidin resistant 5xQUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable QUAS Response Vector

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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KG#797

Plasmid

#110916

Purpose

Expresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neurons

Depositor

Kenneth Miller

Article

Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

Inserts

unc-129 promoter
STRN-1 (Sentryn)-GFP
unc-54 3' control region with 1 intron just upstream and 1 intron in control region

Tags

GFP (contains 3 artificial introns)

Expression

Bacterial

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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gRNA[bTub+DsxF].1046A

Plasmid

#112694

Purpose

express two gRNA targeting bTub & DsxF under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[bTub] & U6.3-gRNA[DsxF] (dsx Fly)

Use

CRISPR

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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plko-tet-on puromycin-shMTH1

Plasmid

#110918

Purpose

To knock down MTH1 (Mut T Homolog 1) after addition of doxycline

Depositor

Priyamvada Rai

Article

Patel et al Oncogene. 2015 May 14;34(20):2586-96. do

Insert

MTH1 (NUDT1 Human)

Use

Lentiviral

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 S334A

Plasmid

#96915

Purpose

Murine Atoh1 with S334A mutation

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Tags

2xFLAG

Expression

Mammalian

Mutation

Serine 334 changed to Alanine

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 S309A

Plasmid

#96916

Purpose

Murine Atoh1 with S309A mutation

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Tags

2xFLAG

Expression

Mammalian

Mutation

Serine 309 changed to Alanine

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 S325A

Plasmid

#96926

Purpose

Murine Atoh1 with S325A mutation

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Tags

2xFLAG

Expression

Mammalian

Mutation

Serine 325 changed to Alanine

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 S328A

Plasmid

#96927

Purpose

Murine Atoh1 with S328A mutation

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Tags

2xFLAG

Expression

Mammalian

Mutation

Serine 328 changed to Alanine

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 S331A

Plasmid

#96928

Purpose

Murine Atoh1 with S331A mutation

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Tags

2xFLAG

Expression

Mammalian

Mutation

Serine 331 changed to Alanine

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Atoh1 S339A

Plasmid

#96929

Purpose

Murine Atoh1 with S339A mutation

Depositor

Albert Edge

Article

Cheng et al J Biol Chem. 2016 Sep 30;291(40):21096-2

Insert

Atoh1 (Atoh1 Mouse)

Tags

2xFLAG

Expression

Mammalian

Mutation

Serine 339 changed to Alanine

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARL5B

Plasmid

#67463

Purpose

Mammalian expression of hArl5b with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5B (ARL5B Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARF5-V5-His6

Plasmid

#67414

Purpose

Expression of hArf5 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF5 (ARF5 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL6

Plasmid

#67380

Purpose

Expression of native hArl6 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL6 (ARL6 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL4C

Plasmid

#67381

Purpose

Expression of native hArl4c in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL8A

Plasmid

#67382

Purpose

Expression of native hArl8 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL14

Plasmid

#67385

Purpose

Expression of native hArl14 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL14 (ARL14 Human)

Expression

Bacterial

Mutation

bp 172 C to T; aa L58F

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL11

Plasmid

#67386

Purpose

Expression of native hArl11 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL11 (ARL11 Human)

Expression

Bacterial

Mutation

bp 261 G to A; silent mutation; bp 442 T to C; aa…

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARFRP1

Plasmid

#67387

Purpose

Expression of native hArfrp in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARFRP1 (ARFRP1 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-SARA

Plasmid

#67389

Purpose

Expression of native hSara in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only