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pMH-06

Plasmid

#158434

Purpose

pBAD33 vector encoding MotA, Dendra2-MotB

Depositor

Francesco Pedaci

Article

Heo et al Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.

Inserts

MotA
MotB

Tags

Dendra2

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-03

Plasmid

#158433

Purpose

pBAD33 vector encoding MotA, yPET-MotB

Depositor

Francesco Pedaci

Article

Heo et al Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.

Inserts

MotA
MotB

Tags

yPET

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pSEVA83-GG

Plasmid

#208016

Purpose

for cloning homology arms, with GFP as counter selection marker

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Insert

msfGFP

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-shCIAP2-B

Plasmid

#44132

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

CIAP2

Use

Lentiviral and RNAi

Available Since

March 29, 2013

Availability

Academic Institutions and Nonprofits only
pWT065c

Plasmid

#107913

Purpose

W2m.3.2-1 plasmid. Contains (TCF-LEF)7-BE3-P2A-Luc and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

(TCF-LEF)7-BE3-P2A-Luc

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pC247

Plasmid

#65890

Purpose

Strong P2 activator plasmid for two-strain system

Depositor

Matthew Bennett

Article

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Insert

RhlI (rhlI )

Tags

LAA tagged

Expression

Bacterial

Promoter

Prhl/lac-s

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAW209

Plasmid

#113188

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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KaiB-FLAG

Plasmid

#41885

Depositor

Michael Rust

Article

Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

Insert

Kai B

Tags

FLAG

Expression

Bacterial

Promoter

t7 promoter

Available Since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
Ypet-RBD

Plasmid

#105291

Purpose

To measure Rho activity by binding and FRET analysis

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

RBD (Rtkn Mouse)

Tags

Ypet

Expression

Mammalian

Mutation

aa encoding 7-89

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-TadA-TadA*

Plasmid

#125661

Purpose

Expresses the TadA-TadA* dimer in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

TadA-TadA*

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO-shcIAP1A

Plasmid

#44129

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

cIAP1

Use

Lentiviral and RNAi

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
NOR gate-V2.0

Plasmid

#44452

Depositor

Drew Endy

Article

Bonnet et al Science. 2013 May 3;340(6132):599-603. d

Insert

NOR-gate

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM12A

Plasmid

#87061

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM12A (Trim12a Mouse)

Expression

Mammalian

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pNR160

Plasmid

#79542

Purpose

Output plasmid: contains 5-state register made up of TP901 and BxbI recognition sites.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Type

Empty backbone

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM12C

Plasmid

#87080

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM12C (Trim12c Mouse)

Expression

Mammalian

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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KvAvs3

Plasmid

#188792

Purpose

Avs3 from Klebsiella variicola PF-1

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

KvAvs3

Expression

Bacterial

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA2-LA-nanoLuc-RA

Plasmid

#128692

Purpose

Donor plasmid that promotes integration of the NanoLuc coding sequence at the apobb.1 locus

Depositor

Steven Farber

Article

Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

Insert

apobb.1 NanoLuc

Use

Multisite-gateway assembly

Tags

NanoLuc

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-FLAG-hG9a

Plasmid

#33023

Depositor

Martin Walsh

Article

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Insert

hG9a

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

5'LTR

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pFF746

Plasmid

#61451

Purpose

aTc-inducible SCRIBE_galK(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(ON)

Use

Synthetic Biology

Promoter

pTetO

Available Since

March 6, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1504

Plasmid

#62358

Purpose

RPS2 HA-TEV-AVI pop-in/pop-out plasmid

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

RPS2 fragment

Use

Yeast targeting

Tags

HA-TEV-AVI

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pDCAF-ABC

Plasmid

#113652

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmA and ndmB and ndmC

Use

Synthetic Biology

Available Since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDA12

Plasmid

#113663

Purpose

Cloning vector for bacterial expression with Pdhfr promoter

Depositor

Miguel Valvano

Article

Aubert et al Infect Immun. 2008 May;76(5):1979-91. do

Type

Empty backbone

Expression

Bacterial

Promoter

Pdhfr

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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NaAtm1

Plasmid

#78308

Purpose

Atm1 type ABC Exporter

Depositor

Douglas Rees

Article

Lee et al Science. 2014 Mar 7;343(6175):1133-6. do

Insert

Atm1 type ABC Exporter

Tags

6x Histidine Tag

Promoter

T7 Promoter

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only
PB-PGK-FKBP-Ztetv-Cit-tvhc-DHFR

Plasmid

#116052

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

FKBP-Ztetv-Cit-tvhc-DHFR

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pJW1514

Plasmid

#62384

Purpose

pCR2.1 linker-tev-linker-3X-FLAG::KanMX

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

tev 3x FLAG

Use

Yeast c-terminal tagging vector

Tags

linker-tev-linker-3xflag

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pNR284

Plasmid

#79550

Purpose

Output plasmid: contains 16-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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p6110 pSG5-HPV31-E8^E2C(HA)

Plasmid

#24741

Depositor

Peter Howley

Article

Powell et al J Virol. 2010 May;84(9):4451-60. doi: 10

Insert

HPV31 E8^E2C (HA)

Tags

HA tag in hinge region

Expression

Mammalian

Available Since

June 30, 2010

Availability

Academic Institutions and Nonprofits only
mTagBFP2-CytERM-N-17

Plasmid

#55289

Purpose

Localization: Endoplasmic Reticulum, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

CytERM

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pYFP.LIC.HXGPRT

Plasmid

#83128

Purpose

Plasmid contains LIC cassette upstream of an in-frame copy of YFP and HXGPRT selectable marker cassette for endogenous tagging on the gene locus.

Depositor

Vernon Carruthers

Article

Huynh et al Eukaryot Cell. 2009 Apr;8(4):530-9. doi:

Insert

YFP

Use

Toxoplasma gondii

Tags

YFP

Promoter

Endogenous Gene

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pFF828

Plasmid

#61456

Purpose

AHL-inducible msd_lacZ(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd_lacZ(ON)

Use

Synthetic Biology

Promoter

pLuxR

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pC220

Plasmid

#65877

Purpose

Medium P2 activator plasmid for two-strain system

Depositor

Matthew Bennett

Article

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Insert

RhlI (rhlI )

Expression

Bacterial

Mutation

LAA tagged

Promoter

Prhl/lac

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pWT050d

Plasmid

#107901

Purpose

W2.5-2 plasmid. Contains PLacO-sgRNA1, PRha-sgRNA6-(sgRNA4-C16T-C18T) and is part of CAMERA 2.5

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PLacO-sgRNA1, PRha-sgRNA6-(sgRNA4-C16T-C18T)

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pWT046e

Plasmid

#107900

Purpose

W2.5-1 plasmid. Contains PTetO-SD8-BE2, PBAD-sgRNA5 (sgRNA3-A8C) and is part of CAMERA 2.5

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PBAD-sgRNA5 (sgRNA3-A8C)

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD3-proD/tdTomato-hph

Plasmid

#120327

Purpose

Bacterial tdTomato labeling

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

tdTomato

Expression

Bacterial

Promoter

proD

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGLx2-SET1

Plasmid

#53256

Purpose

GAL driven LexA-tagged SET1

Depositor

Jean Cook

Article

Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

Insert

SET1

Tags

LexA

Expression

Yeast

Promoter

GAL1/10

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK023

Plasmid

#44860

Depositor

Michael Grunstein

Article

Kitada et al Genes Dev. 2012 Nov 1;26(21):2443-55. do

Insert

ADH4-PURA3-URA3-G8-yEGFP1-CLN2PD-NLSSV40-TURA3-TG

Expression

Yeast

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pWT060e

Plasmid

#107905

Purpose

W2m.0-3, W2m.3-2 plasmid. Contains U6-sgRNA B (CCR5) and is part of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

U6-sgRNA B (CCR5)

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pNR280

Plasmid

#79579

Purpose

A118 attL-attR. GFP expression will stop if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pNR279

Plasmid

#79578

Purpose

BxbI attL-attR. GFP expression will stop if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pNR287

Plasmid

#79580

Purpose

TP901 attL-attR. GFP expression will stop if sites recombine.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1508

Plasmid

#62362

Purpose

pFA6a BirA-mVenus-SSH1::HIS5

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA-mVenus-Ssh1

Tags

BirA-mVenus

Expression

Yeast

Promoter

Gal1

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1516

Plasmid

#62386

Purpose

pFA6a Osm1(M1A)-yoEGFP::LEU2

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

OSM1-EGFP fusion

Tags

EGFP

Expression

Yeast

Mutation

Methionine 1 to Alanine

Promoter

Adh1p

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
eDHFR-E3

Plasmid

#236113

Purpose

Half of chemically inducible GFE3. When co-expressed with 236112 can degrade inhibitory synapses in presence of TH. (trimethoprim-HaloTag ligand).

Depositor

Don Arnold

Article

Bareghamyan et al eLife. 2025 Apr 29;13:RP103757. doi: 10.

Insert

eDHFR-E3

Expression

Mammalian

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLB025

Plasmid

#234634

Purpose

pET-28a(+) based plasmid for expression of H16_A1358 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_A1358

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SJZ2-TRP1

Plasmid

#228256

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker TRP1 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

TRP1

Expression

Yeast

Promoter

BBC1 S.c.

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SJZ10-LEU2

Plasmid

#228262

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker LEU2 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

LEU2

Expression

Yeast

Promoter

LEU2 K.l.

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHA9

Plasmid

#223642

Purpose

pUC18 plasmid with rpsM promoter

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pZLrhaB2plus

Plasmid

#211787

Purpose

pSCrhaB2plus with modifications of resistance cassette to CmR

Depositor

Silvia Cardona

Article

Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

Insert

CmR

Expression

Bacterial

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN099

Plasmid

#220044

Purpose

Mammalian expression of type III-A crRNA from Streptococcus thermophilus

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

crRNA

Expression

Mammalian

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC18T-mini-Tn7T-hph-lacZ

Plasmid

#218370

Purpose

Empty backbone for lacZ fusions

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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