Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 22,079 results for: his

Showing: 5451 - 5500 of 22079 results

  1. pColdII-SIRPT1-Eptesicus fuscus

    Plasmid
    #78113
    Purpose
    Nt His-tag Eptesicus fuscus Sacsin 89-1368
    Depositor
    Kalle Gehring
    Insert
    Sacsin Eptesicus fuscus 89-1368
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78113-plasmid-map-sequence-id-145659

    Enlarge

  2. pColdII-SIRPT1-Bubalus bubalis

    Plasmid
    #75350
    Purpose
    Nt His-tag Bubalus bubalis Sacsin 86-1364
    Depositor
    Kalle Gehring
    Insert
    Sacsin Bubalus bubalis 86-1364
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75350-plasmid-map-sequence-id-142906

    Enlarge

  3. pColdII-SIRPT1-Latimeria chalumnae

    Plasmid
    #75356
    Purpose
    Nt His-tag Latimeria chalumnae Sacsin 94-1368
    Depositor
    Kalle Gehring
    Insert
    Sacsin Latimeria chalumnae 94-1368
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75356-plasmid-map-sequence-id-142889

    Enlarge

  4. pColdII-SIRPT1-Equus caballus

    Plasmid
    #78114
    Purpose
    Nt His-tag Equus caballus Sacsin 40-1317
    Depositor
    Kalle Gehring
    Insert
    Sacsin Equus caballus 40-1317
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78114-plasmid-map-sequence-id-145641

    Enlarge

  5. OsMATE

    Plasmid
    #117093
    Purpose
    protein expression in yeast
    Depositor
    Geoffrey Chang
    Insert
    AtTPT (ORF-HIS)
    Use
    Tags
    His
    Expression
    Yeast
    Mutation
    Promoter
    AOX
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    117093-plasmid-map-sequence-id-224955

    Enlarge

  6. pColdII-SIRPT1-Phoenix dactylifera

    Plasmid
    #78117
    Purpose
    Nt His-tag Phoenix dactylifera Sacsin 1-1295
    Depositor
    Kalle Gehring
    Insert
    Sacsin Phoenix dactylifera 1-1295
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78117-plasmid-map-sequence-id-145624

    Enlarge

  7. pColdII-SIRPT1-Nelumbo nucifera

    Plasmid
    #75358
    Purpose
    Nt His-tag Nelumbo nucifera Sacsin 1-1313
    Depositor
    Kalle Gehring
    Insert
    Sacsin Nelumbo nucifera 1-1313
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75358-plasmid-map-sequence-id-142884

    Enlarge

  8. Pv6PGDH-pNic-Bsa4

    Plasmid
    #231102
    Purpose
    Expresses Plasmodium vivax 6-phosphogluconate dehydrogeanse under T7 with HIS tag
    Depositor
    Josh Beckham
    Insert
    6-phosphogluconate dehydrogenase
    Use
    Tags
    HIS tag
    Expression
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    231102-plasmid-map-sequence-id-462632

    Enlarge

  9. pColdII-SIRPT1-Leptonychotes weddellii

    Plasmid
    #78115
    Purpose
    Nt His-tag Leptonychotes weddellii Sacsin 89-1367
    Depositor
    Kalle Gehring
    Insert
    Sacsin Leptonychotes weddellii 89-1367
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    78115-plasmid-map-sequence-id-145636

    Enlarge

  10. pColdII-SIRPT1-Chlorocebus sabaeus Sacsin

    Plasmid
    #78112
    Purpose
    Nt His-tag Chlorocebus sabaeus Sacsin 86-1375
    Depositor
    Kalle Gehring
    Insert
    Sacsin Chlorocebus sabaeus 86-1375
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78112-plasmid-map-sequence-id-145660

    Enlarge

  11. pColdII-SIRPT1-Sarcophilus harrisii

    Plasmid
    #75352
    Purpose
    Nt His-tag Sarcophilus harrisii Sacsin 90-1369
    Depositor
    Kalle Gehring
    Insert
    Sacsin Sarcophilus harrisii 90-1369
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75352-plasmid-map-sequence-id-142897

    Enlarge

  12. pColdII-SIRPT1-Serinus canaria

    Plasmid
    #75353
    Purpose
    Nt His-tag Serinus canaria Sacsin 90-1368
    Depositor
    Kalle Gehring
    Insert
    Sacsin Serinus canaria 90-1368
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75353-plasmid-map-sequence-id-142896

    Enlarge

  13. pColdII-SIRPT1-Pelodiscus sinensis

    Plasmid
    #75354
    Purpose
    Nt His-tag Pelodiscus sinensis Sacsin 90-1379
    Depositor
    Kalle Gehring
    Insert
    Sacsin Pelodiscus sinensis 90-1379
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75354-plasmid-map-sequence-id-142903

    Enlarge

  14. pColdII-SIRPT1-Cucumis sativus

    Plasmid
    #75357
    Purpose
    Nt His-tag Cucumis sativus Sacsin 1-1292
    Depositor
    Kalle Gehring
    Insert
    Sacsin Cucumis sativus 1-1292
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75357-plasmid-map-sequence-id-142885

    Enlarge

  15. pColdII-SIRPT1-Jatropha curcas

    Plasmid
    #75359
    Purpose
    Nt His-tag Jatropha curcas Sacsin 1-1304
    Depositor
    Kalle Gehring
    Insert
    Sacsin Jatropha-curcas 1-1304
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75359-plasmid-map-sequence-id-142713

    Enlarge

  16. pET28a hMDH2

    Plasmid
    #204230
    Purpose
    Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    hMDH2 (MDH2 Human)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does not include transit peptide and includes a M…
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204230-plasmid-map-sequence-id-413333

    Enlarge

  19. pCoofy50

    Plasmid
    #55189
    Purpose
    Mammalian expression vector for parallel SLIC cloning containing N-terminal HA, 8x His and FLAG tags. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Use
    Tags
    8x His, FLAG, HA, PreScission site (3C protease s…
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Aug. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    55189-plasmid-map-sequence-id-85571

    Enlarge

  20. pEcRNAP6

    Plasmid
    #128940
    Purpose
    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z
    Depositor
    Seth Darst
    Article
    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:
    Inserts
    RpoA (rpoA E. coli)
    RpoB
    RpoC-ppx(His)10
    RpoZ
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    PreScission protease site (GE Healthcare; LEVLFQG…
    Promoter
    T7
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128940-plasmid-map-sequence-id-254597

    Enlarge

  21. pet30b-7M SrtA

    Plasmid
    #51141
    Purpose
    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity
    Depositor
    Hidde Ploegh
    Insert
    S.aureus SrtA 7M
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    P94R, E105K, E108Q, D160N, D165A, K190E, K196T
    Promoter
    T7
    Available since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    51141-plasmid-map-sequence-id-211598

    Enlarge

  22. pColdII-SIRPT1-Xenopus (Silurana) tropicalis

    Plasmid
    #75355
    Purpose
    Nt His-tag Xenopus (Silurana) tropicalis Sacsin 87-1376
    Depositor
    Kalle Gehring
    Insert
    Sacsin Xenopus (Silurana) tropicalis 87-1376
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75355-plasmid-map-sequence-id-142888

    Enlarge

  23. NM-01

    Plasmid
    #69799
    Purpose
    Bacterial expression of Cas9 with His, MBP, and Strep tags
    Depositor
    Gerard Cagney
    Insert
    Cas9
    Use
    Tags
    HIS, MBP, and StrepII
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine (D10A) and Histidine 840 …
    Promoter
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  24. ALDO-1_KS

    Plasmid
    #224157
    Purpose
    IPTG-inducible expression of C. elegans protein ALDO-1 with a His tag
    Depositor
    Katherine Walstrom
    Insert
    ALDO-1
    Use
    Tags
    LeuGlu-6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    224157-plasmid-map-sequence-id-444332

    Enlarge

  25. thoc5.018.389

    Plasmid
    #137266
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    thoc5 (THOC5 Human)
    Use
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    018-389
    Promoter
    Available since
    Feb. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    137266-plasmid-map-sequence-id-323966

    Enlarge

  26. NKAP.001.140

    Plasmid
    #137206
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    NKAP (NKAP Human)
    Use
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    001-140
    Promoter
    Available since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    137206-plasmid-map-sequence-id-322594

    Enlarge

  27. pColdII-SIRPT1-Ceratotherium-simum-simum

    Plasmid
    #78111
    Purpose
    Nt His-tag Ceratotherium-simum-simum Sacsin 89-1366
    Depositor
    Kalle Gehring
    Insert
    Sacsin Ceratotherium-simum-simum 89-1366
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78111-plasmid-map-sequence-id-145661

    Enlarge

  28. pColdII-SIRPT1-Mustela putorius furo

    Plasmid
    #78116
    Purpose
    Nt His-tag Mustela putorius furo Sacsin 94-1381
    Depositor
    Kalle Gehring
    Insert
    Sacsin Mustela putorius furo 94-1381
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78116-plasmid-map-sequence-id-145629

    Enlarge

  30. pBad-mKalama1

    Plasmid
    #14892
    Purpose
    Bacterial expression of mKalama1 blue fluorescent protein
    Depositor
    Robert Campbell
    Article
    Ai et al Biochemistry. 2007 May 22;46(20):5904-10
    Insert
    mKalama1
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    The fluorescent protein was inserted between XhoI…
    Promoter
    Available since
    May 15, 2007
    Availability
    Academic Institutions and Nonprofits only

  31. pCoofy44

    Plasmid
    #55185
    Purpose
    Insect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Use
    Tags
    10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…
    Expression
    Bacterial
    Mutation
    Promoter
    pPH (Polyhedrin promoter)
    Available since
    July 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    55185-plasmid-map-sequence-id-85559

    Enlarge

  32. pET28a hMDH1v3 (+TEV)

    Plasmid
    #204229
    Purpose
    Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    hMDH1v3 (MDH1 Human)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Inserted a G after ATG to maintain kozak site
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204229-plasmid-map-sequence-id-413334

    Enlarge

  35. pBad-mAmetrine

    Plasmid
    #18083
    Purpose
    Bacterial expression of mAmetrine fluorescent protein
    Depositor
    Robert Campbell
    Article
    Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1
    Insert
    mAmetrine fluorescent proteins
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    The fluorescent protein was inserted between XhoI…
    Promoter
    Available since
    May 29, 2008
    Availability
    Academic Institutions and Nonprofits only

  36. pMB-Tl-ADPHK

    Plasmid
    #237469
    Purpose
    Expression of His-tagged Thermococcus litoralis ADP hexokinase
    Depositor
    Michael Baker
    Insert
    ADP hexokinase
    Use
    Tags
    C-terminal 10 His-tag and N-terminal strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    237469-plasmid-map-sequence-id-473992

    Enlarge

  37. KPC2-pNic-Bsa4

    Plasmid
    #231082
    Purpose
    expresses Klebsiella pneumoniae carbapenemase-2 (KPC-2) under T7 with His tag
    Depositor
    Josh Beckham
    Insert
    carbapenemase-2 (kpc2 )
    Use
    Tags
    6x His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    231082-plasmid-map-sequence-id-462636

    Enlarge

  38. ADH5_KG

    Plasmid
    #224150
    Purpose
    IPTG-inducible expression of C. elegans protein ADH-5 with a His tag
    Depositor
    Katherine Walstrom
    Insert
    ADH-5 (adh-5 Nematode)
    Use
    Tags
    LeuGlu-6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    224150-plasmid-map-sequence-id-444363

    Enlarge

  39. Francisella tularensis enoyl reductase

    Plasmid
    #231104
    Purpose
    Expresses FabI under T7 with HIS tag
    Depositor
    Josh Beckham
    Insert
    Enoyl-acyl carrier protein reductase
    Use
    Tags
    6x HIS, GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231104-plasmid-map-sequence-id-465515

    Enlarge

  40. FtHAP-pNIC-Bsa4

    Plasmid
    #86778
    Purpose
    Expresses Francisella tularensis Histidine Acid Phosphatase under T7 with HIS tag
    Depositor
    Josh Beckham
    Insert
    Histidine Acid Phosphatase
    Use
    Tags
    6x His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    86778-plasmid-map-sequence-id-462633

    Enlarge

  44. pTDpelB-NTwinStrep

    Plasmid
    #45940
    Purpose
    use to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequence
    Depositor
    Thorben Dammeyer
    Article
    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.
    Type
    Empty backbone
    Use
    Tags
    6xHis, PelB, and Twin-Strep
    Expression
    Bacterial
    Mutation
    Promoter
    Trc (lactose/IPTG inducible)
    Available since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    45940-plasmid-map-sequence-id-64653

    Enlarge

  46. pCT02

    Plasmid
    #212152
    Purpose
    Plasmid expressing 6x His tagged FAST-PETase under inducible promoter PT7 control.
    Depositor
    Kang Zhou
    Insert
    FAST-PETase
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    S121E, D186H, R224Q, N233K, R280A
    Promoter
    PT7
    Available since
    March 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    212152-plasmid-map-sequence-id-424774

    Enlarge

  47. pFB-CT10HF-LIC

    Plasmid
    #39191
    Purpose
    SGC Baculovirus transfer vector with C-terminal His tag and FLAG tag, preceded by a TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection
    Depositor
    Nicola Burgess-Brown
    Type
    Empty backbone
    Use
    Baculovirus
    Tags
    TEV-His-FLAG
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39191-plasmid-map-sequence-id-136169

    Enlarge

  48. WoFabI-pNIC-Bsa4

    Plasmid
    #240437
    Purpose
    Expresses enoyl-ACP reductase FabI [Wolbachia endosymbiont of Brugia malayi] under T7 with HIS tag in bacterial cells)
    Depositor
    Josh Beckham
    Insert
    Enoyl-acyl carrier protein reductase
    Use
    Tags
    6x His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    240437-plasmid-map-sequence-id-481073

    Enlarge

  49. PRPF4.001.169.S34E

    Plasmid
    #137643
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene, with point mutation, in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Use
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    001-169-S34E
    Promoter
    Available since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137643-plasmid-map-sequence-id-381376

    Enlarge

  50. PRPF4.211.522.gst

    Plasmid
    #137650
    Purpose
    Expresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Use
    Tags
    GST-(His)6-thrombin
    Expression
    Bacterial
    Mutation
    211-522-gst
    Promoter
    Available since
    Nov. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    137650-plasmid-map-sequence-id-377729

    Enlarge

Showing: 5451 - 5500 of 22079 results