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We narrowed to 7,002 results for: tet

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Showing: 5451 - 5500 of 7002 results

  10. TRIPZ-CTID195-GSQ-UBnc-PURO

    Plasmid
    #208037
    Purpose
    Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    Ubnc (UBC Human)
    Use
    Lentiviral
    Tags
    Myc, C-terminal Split-TurboID
    Mutation
    L73P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208037-plasmid-map-sequence-id-410268

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  12. TRE-RXRa-Myc; pGK-H2B-RFP

    Plasmid
    #242887
    Purpose
    For lentiviral overexpression of Myc-tagged human RXRa coupled to H2B-RFP (in the opposite direction)
    Depositor
    Elaine Fuchs
    Article
    Stewart et al Nature. 2024 Sep;633(8029):407-416. doi:
    Insert
    Rxra (RXRA Human)
    Use
    Lentiviral
    Tags
    H2B-mRFP
    Expression
    Mammalian
    Mutation
    Human RXRa cDNA was PCR-amplified from pSV-Sport-…
    Promoter
    Tetracycline response element upstream of a minim…
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    242887-plasmid-map-sequence-id-484988

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  16. TRIPZ-CTID195-GSQ-SUMO1nc-PURO

    Plasmid
    #208035
    Purpose
    Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    SUMO1nc (SUMO1 Human)
    Use
    Lentiviral
    Tags
    Myc, C-terminal Split-TurboID
    Mutation
    Q94P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208035-plasmid-map-sequence-id-410209

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  17. pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

    Plasmid
    #235307
    Purpose
    Lentiviral expression of ComMAND open-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)
    Depositor
    Kate Galloway
    Article
    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1
    Insert
    EGFP-Fmr1 (Fmr1 Mouse)
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    TRE3G (3rd-generation Tet system)
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235307-plasmid-map-sequence-id-472801

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  18. pLentiX1-[TRE3G-FXN-P2A-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-EFS-rtTA-P2A-mGL-WPRE

    Plasmid
    #235304
    Purpose
    Lentiviral expression of ComMAND open-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)
    Depositor
    Kate Galloway
    Article
    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1
    Insert
    FXN-P2A-mRuby2 (FXN Human)
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G (3rd-generation Tet system)
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235304-plasmid-map-sequence-id-472752

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  19. pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF4x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

    Plasmid
    #235308
    Purpose
    Lentiviral expression of ComMAND closed-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)
    Depositor
    Kate Galloway
    Article
    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1
    Insert
    EGFP-Fmr1 (Fmr1 Mouse)
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    TRE3G (3rd-generation Tet system)
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235308-plasmid-map-sequence-id-472753

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  20. pLentiX1-[TRE3G-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-EFS-rtTA-P2A-mGL-WPRE

    Plasmid
    #235305
    Purpose
    Lentiviral expression of ComMAND closed-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)
    Depositor
    Kate Galloway
    Article
    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1
    Insert
    FXN-P2A-mRuby2 (FXN Human)
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G (3rd-generation Tet system)
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235305-plasmid-map-sequence-id-472751

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  21. pAU003 - pTRE3G-Cterm_Nterm_switch_CTCF-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid
    #156430
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    Cterm_Nterm_switched
    Available Since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    156430-plasmid-map-sequence-id-310203

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  22. Desmoplakin I no force control (F40-based)

    Plasmid
    #119187
    Purpose
    The no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between mTFP1 and mEYFP that are tension-independent.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakinhuman Desmoplakin I (1-1945)-[mTFP1-F40-mEYFP] (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    truncation after aa1945
    Promoter
    TCE
    Available Since
    Dec. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    119187-plasmid-map-sequence-id-230534

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  23. pPD177.62

    Plasmid
    #1701
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    pes10UAS-270, pes10Tsa, IVSA, SV40NLS, TetRN, Tet…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1701-plasmid-map-sequence-id-307

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  24. pPD177.53

    Plasmid
    #1702
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    pes10UAS-270, pes10Tsa, IVSA, SV40NLS, TetRN, Tet…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1702-plasmid-map-sequence-id-308

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  26. pHL 233

    Plasmid
    #37754
    Depositor
    Han Lim
    Article
    Hussein et al Nucleic Acids Res. 2012 May 22.
    Inserts
    ptet_ompC_gfp
    pLac_st7 TetR_mCherry
    Use
    Synthetic Biology; E. coli
    Tags
    gfp and mCherry
    Promoter
    pLac and ptet
    Available Since
    Aug. 10, 2012
    Availability
    Academic Institutions and Nonprofits only

  28. pCCM

    Plasmid
    #162708
    Purpose
    Second CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM cluster
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    Second CCM operon cloned from H. neapolitanus
    Expression
    Bacterial
    Promoter
    PLtet0-1 promoter
    Available Since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162708-plasmid-map-sequence-id-324195

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  32. pKS014

    Plasmid
    #65467
    Purpose
    pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and beta-galactosidase
    Depositor
    Keith Tyo
    Article
    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do
    Insert
    Ntag-beta galactosidase
    Use
    Synthetic Biology
    Tags
    FLAG
    Expression
    Bacterial
    Promoter
    pl-TetO
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  33. pTrc99S-ssDsbA-TTlight-4xDQNAT

    Plasmid
    #128402
    Purpose
    Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Tetanus toxin light chain domain
    Tags
    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…
    Expression
    Bacterial
    Mutation
    Inactivating E234A mutation
    Promoter
    trc
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128402-plasmid-map-sequence-id-263357

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  34. pCB’ CbbL Y72R

    Plasmid
    #162707
    Purpose
    Carboxysome operon with point mutation to CbbL, prk; Substitution of CsoS2-binding tyrosine with arginine prevents rubisco encapsulation creating rubisco-less carboxysomes
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    pHnCB10 derived carboxysome operon, prk
    Expression
    Bacterial
    Mutation
    CbbL Y72R; Substitution of CsoS2-binding tyrosine…
    Promoter
    PLtet0-1 promoter
    Available Since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    162707-plasmid-map-sequence-id-326996

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  40. pCas9CR5-NG

    Plasmid
    #120228
    Purpose
    pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120228-plasmid-map-sequence-id-233263

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Showing: 5451 - 5500 of 7002 results