We narrowed to 23,099 results for: CRISPR
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Plasmid#90991PurposeNAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.DepositorInsertgRNA conserved, FRT, DS_HIS1
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS SK mCherryROSAegfp
Plasmid#54322PurposeEncodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.DepositorInsertmCherry and EGFP
UseCRISPR, Mouse Targeting, and TALENTagsExpressionMammalianMutationPromoterCMVAvailable SinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cas9-2A-EGFP
Plasmid#167928PurposeDoxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.DepositorInsertT2A-EGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotertTRE, hPGKAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-sgRNA(MS2)
Plasmid#102560PurposePiggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonTagsExpressionMammalianMutationPromoterU6Available SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas
Plasmid#62225PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRDepositorHas ServiceCloning Grade DNAInsertcas9
UseCRISPR; Lambda red recombinaseTagsExpressionBacterialMutationPromoternative cas9 promoterAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBECAb-apr
Plasmid#122001PurposeA cytidine deaminase-mediated base-editing plasmid in Acinetobacter baumanniiDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable SinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330S-2-PITCh
Plasmid#63670PurposeExpresses Cas9 nuclease and the PITCh-gRNADepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable SinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRU55
Plasmid#167684PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::asCpf1
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Cas9-EGFP
Plasmid#159126PurposeExpresses active Cas9 fused with EGFP for fluorescence microscopyDepositorInsertCas9 fused to EGFP
UseCRISPR and RetroviralTagsEGFPExpressionMammalianMutationPromoterMSCV LTR promoterAvailable SinceDec. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-bacteria
Plasmid#44251PurposeExpression of customizable guide RNA (gRNA) for bacterial gene knockdownDepositorInsertguide RNA (bacteria)
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)Available SinceApril 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-T2A-GFP
Plasmid#53190Purpose3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFPDepositorInserthumanized Cas9 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationPromoterhUbCAvailable SinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ150
Plasmid#69301PurposeGateway entry vector with pcoCas9 (Plant codon optimized)DepositorInsertpcoCas9 (plant codon-optimized)
UseCRISPRTags3XFLAGExpressionMutationNLS fusionPromoterAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330-LMNA-gRNA1
Plasmid#122507Purposeexpresses WT spCas9 and a chimeric gRNA targeting human LMNADepositorInsertLMNA-gRNA1
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available SinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
sgOpti
Plasmid#85681PurposeLentiviral vector to express sgRNAs. The cloning site is BsmBI.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS159
Plasmid#122378PurposeCRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
ampl43
Plasmid#161830PurposepRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterTEF1Available SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC0042-PguCas13b crRNA backbone
Plasmid#103853PurposeFor cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caacDepositorInsertU6-PguCas13b DR-BbsI-BbsI-polyT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable SinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
p201N Cas9
Plasmid#59175PurposeCas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
nptII
UseCRISPRTagsExpressionMutationPromoter2x35S and StUbi-3PAvailable SinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-dU6:3gRNA
Plasmid#49410Purposeexpression of gRNA under control of the Drosophila U6:3 promoterDepositorInsertdU6-3:gRNA
UseCRISPRTagsExpressionInsectMutationPromoterdU6-3Available SinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFC333
Plasmid#87844PurposeAMA1 plasmid with Aspergillus optimized Cas9 and ble selection markerDepositorInsertsCas9
ble (bleomycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only