We narrowed to 15,732 results for: LEA

1. pcDNA3-TRAIL-R3

   Plasmid

   #61384

   Purpose

   Expresses TRAIL receptor 3

   Depositor

   Francis Chan

   Article

   Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

   Insert

   TRAIL receptor 3 (TNFRSF10C Human)
   

   Tags

   HA tag and TNF receptor leader peptide

   Expression

   Mammalian

   Mutation

   TRAIL-R3 leader peptide (aa1-29) replaced with TN…

   Available Since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA-GFP-EBA175-Flag

   Plasmid

   #154970

   Purpose

   Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfEBA175
   

   Tags

   GFP and signal peptide (residues 1 to 32) from TG…

   Expression

   Mammalian

   Mutation

   insert codes for EBA175 residues 1284 to 1462

   Promoter

   CMV

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRD421

   Plasmid

   #163112

   Purpose

   Production of His6_TEV-cleavable-linker_mEos3.2_H-NSdbd in BL21 (DE3) pLysS

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   His-tag_TEV-cleavable-linker_mEos3.2_H-NSdbd
   

   Tags

   His-tag with TEV-cleavable linker

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. FASTHDR-Cterm-NanoLuc-Puromycin

   Plasmid

   #167209

   Purpose

   Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   NanoLuc

   Expression

   Mammalian

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKS7107

   Plasmid

   #89051

   Purpose

   Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   Nm crRNA
   Nm tracrRNA
   hNmCas9
   

   Use

   CRISPR

   Tags

   HA tag, NLS, and SV40 NLS

   Promoter

   EF1a and human U6

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-PM-InPAkt

   Plasmid

   #181915

   Purpose

   Indicator of Phosphoinositides using Akt; targeted to the plasma membrane.

   Depositor

   Jin Zhang

   Article

   Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

   Insert

   pm-InPAkt
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Contains the following mutations with respect to …

   Promoter

   CMV

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pExpreS2-1-CR-PA-10H-SIII

   Plasmid

   #175447

   Purpose

   HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

   Depositor

   Wyatt Yue

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Tags

   BiP Secretion Peptide and Thrombin-Protein A-10Hi…

   Expression

   Insect

   Promoter

   Fused Actin-HSP70

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. NET37 pEGFP-N2 (607)

   Plasmid

   #61990

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   NET37 (KIAA1161 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

9. NET50 pmRFP-N2 (489)

   Plasmid

   #61983

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

   Insert

   NET50 (DHRS7 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

10. NET97 pmRFP-N2 (641)

    Plasmid

    #61994

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    AADACL1 (NCEH1 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pFastBac-HS-NLRC4Δcard

    Plasmid

    #177276

    Purpose

    Purification of mouse NLRC4 (90-1024) from insect cells

    Depositor

    Hao Wu

    Article

    Zhang et al Science. 2015 Oct 23;350(6259):404-9. do

    Insert

    NLRC4 (Nlrc4 Mouse)
    

    Tags

    HIS-SUMO-PreScission

    Expression

    Insect

    Mutation

    I586T, N761S, M885V, K915R and F972L- please see …

    Promoter

    polyhedrin

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCLASP_Crz1(19A)_CLASP_RGS2membrane

    Plasmid

    #133085

    Purpose

    Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    Crz1*-CLASP
    

    Expression

    Bacterial and Yeast

    Promoter

    pADH1

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. NET37 pmRFP-N2 (450)

    Plasmid

    #61980

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET37 (KIAA1161 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pTE3987

    Plasmid

    #137716

    Purpose

    Expresses dLbCas12a-RVRR-BE in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    dLbCas12a-RVRR-BE
    

    Tags

    6xHis, APOBEC-1, SV40 NLS, and UGI

    Expression

    Mammalian

    Mutation

    G532A, K538V, Y542R, K595R

    Promoter

    CMV

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-NES-tdTomato-LINuS-WPRE

    Plasmid

    #159894

    Purpose

    AAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signal

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Biochem Biophys Res Commun. 2020 Jan 29;

    Insert

    NES-tdTomato-LINuS
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF1α promoter

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-JNKsub(TA)DD-YPet-NES

    Plasmid

    #84634

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    JNKsub(TA)DD-YPet-NES
    

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. TAPBPL pEGFP-C3 (1765)

    Plasmid

    #62055

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

    Insert

    TAPBPL (TAPBPL Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

18. Hy_pMT Flag-UAP56 MUT

    Plasmid

    #110124

    Purpose

    Expresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    UAP56 (DDX39B Human)
    

    Tags

    Flag

    Expression

    Insect

    Mutation

    4 mutations (KSLN motif changed to RSFS)

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. ADAP1

    Plasmid

    #163905

    Purpose

    Full length mouse ADAP1 for purification from insect cells

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    ADAP1 (Adap1 Mouse)
    

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-4T3-ZO-2-1st-PDZ-delta-NLS

    Plasmid

    #27425

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Deletion of NLS-Nuclear Localization Signal: Nucl…

    Available Since

    May 18, 2011

    Availability

    Academic Institutions and Nonprofits only