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We narrowed to 15,566 results for: LEA

Showing: 2181 - 2200 of 15566 results

  2. pcDNA3.1-mPCSK3 H194L

    Plasmid
    #122689
    Purpose
    Expresses mutated mouse PCSK3 in mammalian cells (histidine at position 194 mutated to leucine via site directed mutagenesis)
    Depositor
    Liming Pei
    Article
    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:
    Insert
    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Histidine 194 to Leucine
    Promoter
    Available since
    May 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    122689-plasmid-map-sequence-id-244742

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  4. pcDNA3.1-mPCSK3 S368A

    Plasmid
    #122680
    Purpose
    Expresses mutated mouse PCSK3 in mammalian cells (serine at position 368 mutated to alanine via site directed mutagenesis)
    Depositor
    Liming Pei
    Article
    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:
    Insert
    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Serine 368 to Alanine
    Promoter
    Available since
    May 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    122680-plasmid-map-sequence-id-244743

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  6. pRSET-XS-VWF

    Plasmid
    #64847
    Purpose
    Expresses aa1594–1670 of VWF A2 domain flanked by Venus and Cerulean and tagged with 7X His at C terminus
    Depositor
    Sriram Neelamegham
    Article
    Dayananda et al Anal Biochem. 2011 Mar 15;410(2):206-13.
    Insert
    Venus_VWF A2 domain aa 1594-1670_Cerulean_TEV_7X His (VWF Human, Synthetic)
    Use
    Tags
    7X His, Cerulean, and Venus
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    64847-plasmid-map-sequence-id-109340

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  7. pMTB2-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80067
    Purpose
    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Beta-actin2
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80067-plasmid-map-sequence-id-152621

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  8. pcDNA3-mCherry-TSC2(AA,3Q)

    Plasmid
    #192444
    Purpose
    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity.
    Depositor
    Jin Zhang
    Article
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Insert
    mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q ) (TSC2 Human)
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …
    Promoter
    CMV
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    192444-plasmid-map-sequence-id-379131

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  11. pMT-MCS-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80059
    Purpose
    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80059-plasmid-map-sequence-id-152593

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  12. pSN-A112C-GpA-G83I

    Plasmid
    #191239
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    Use
    Tags
    nuclease A from S. aureus fused to the TM domain …
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and Glycin…
    Promoter
    T7 promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191239-plasmid-map-sequence-id-380471

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  14. hTln1-R12DD-SCLS

    Plasmid
    #191443
    Purpose
    expresses a variant of human talin1 domains R12-DD, with a deletion between K2375 and A2397
    Depositor
    Ben Goult
    Article
    Elefant et al JCI Insight. 2024 Dec 20;9(24):e173664.
    Insert
    Human Talin1 R12DD (TLN1 Human)
    Use
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Mutation
    deletion between residues K2375 and A2397
    Promoter
    Available since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191443-plasmid-map-sequence-id-380513

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  20. MSNA-c028

    Plasmid
    #175470
    Purpose
    Protein expression in bacterial cells. FERM domain, M1-E346. Contains H288A point mutation that reduces binding to CD44.
    Depositor
    Opher Gileadi
    Article
    Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10
    Insert
    MSN (MSN Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    H288A
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175470-plasmid-map-sequence-id-353161

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Showing: 2181 - 2200 of 15566 results