We narrowed to 8,313 results for: reporter

1. GB4542

   Plasmid

   #215261

   Purpose

   Module for the expression of the N. nambi HISPS gene under the regulation of the copper inducible CBS4x:mDFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   CBS4x:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. GB4401

   Plasmid

   #215252

   Purpose

   Module for the expression of the N. nambi HISPS gene under the regulation of PNOS and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   SF-PNOS:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF-ABEmax

   Plasmid

   #164415

   Purpose

   EF1alpha promoter + ABEmax (derived from #112095). Mammalian cell adenosine base editor expression vector.

   Depositor

   Xiao Wang

   Article

   Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

   Insert

   ABEmax
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMIII-Neo-BFP

   Plasmid

   #153426

   Purpose

   Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

   Depositor

   Maki Nakayama

   Article

   Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

   Insert

   mTagBFP2
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 11, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pDNR-SpCas9

   Plasmid

   #80425

   Purpose

   Used for in vitro transcription of SpCas9 mRNA.

   Depositor

   Melissa Little, Sara Howden

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   none

   Mutation

   codon optimized for human

   Promoter

   T7

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDT-sgRNA-XMAS-1x

   Plasmid

   #164413

   Purpose

   Dual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

   Depositor

   Xiao Wang

   Article

   Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

   Insert

   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. TetO-FUW-VdC9BV

   Plasmid

   #62195

   Purpose

   Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes

   Depositor

   Kam Leong

   Article

   Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7

   Insert

   VP64dCas9BFPVP64
   

   Use

   Lentiviral

   Tags

   Two VP64s tagged to dCas9 fused to BFP

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive) Cas9 (dCas9)

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5_tAKARβ

   Plasmid

   #119914

   Purpose

   Mammalian expression of the tAKARβ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

   Depositor

   Haining Zhong

   Article

   Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

   Insert

   tAKARβ
   

   Expression

   Mammalian

   Available Since

   Feb. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB_iNEUROD1_P2A_GFP_Puro

   Plasmid

   #168803

   Purpose

   PiggyBac transposon construct with doxycycline inducible NEUROD1 + GFP expression

   Depositor

   Prashant Mali

   Article

   Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

   Insert

   NEUROD1 (NEUROD1 Human, Synthetic)
   

   Use

   Synthetic Biology; Piggybac

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3-basic-hRARβP-FL-luciferase

    Plasmid

    #135447

    Purpose

    Human full-length promoter of retinoic acid receptor-beta gene in pGL3-Basic-luciferase plasmid vector

    Depositor

    Catharine Ross

    Article

    Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

    Insert

    Full-length promoter of human retinoic acid receptor-beta gene
    

    Promoter

    Human RARβ promoter

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGREAT2

    Plasmid

    #170890

    Purpose

    GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_NOSt+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    p35SoTMV

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a(+)::MBP-NL3F10H

    Plasmid

    #141291

    Purpose

    MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    MBP-NLUC3F10H
    

    Use

    Luciferase

    Tags

    Flag (x3), His (x10), and MBP

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGREAT1

    Plasmid

    #170889

    Purpose

    GoldenBraid Double Transcriptional Unit - pNOS_E-Luc_NOSt+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    pNOS

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. A3Bctd-max

    Plasmid

    #198888

    Purpose

    Express A3Bctd-max BE4max-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
    

    Tags

    6x His and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNLF1-C_hCOL4A5-Nluc_R1683X

    Plasmid

    #198124

    Purpose

    Express hCOL4A5_R1683X in mammalian cells

    Depositor

    Jeffrey Miner

    Article

    Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

    Insert

    COL4A5 (COL4A5 Human)
    

    Tags

    Nluc

    Expression

    Mammalian

    Mutation

    R1683X

    Promoter

    CMV, beta-globin/IgG chimeric intron

    Available Since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRK5_tAKARα

    Plasmid

    #119913

    Purpose

    Mammalian expression of the tAKARα sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARα
    

    Expression

    Mammalian

    Available Since

    March 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONOR-tagBFP-PSM-EGFP

    Plasmid

    #100603

    Purpose

    Donor scaffold with EGFP positive selection module, and tagBFP negative selection module

    Depositor

    Jens Schwamborn

    Article

    Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    EGFP-T2A-Puro and tagBFP

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGREAT26

    Plasmid

    #170914

    Purpose

    GoldenBraid Double Transcriptional Unit - pEstradiol_Inducible_E-Luc_NtEUt+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    pEstradiol_Inducible

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBAD/HisB-Medium-FT

    Plasmid

    #31917

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Medium-FT
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

    Promoter

    araB

    Available Since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJSK418

    Plasmid

    #205131

    Purpose

    Expression of GFP-2xFYVE in Dictyostelium

    Depositor

    Jason King

    Article

    Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

    Insert

    2xFYVE (from human Hrs) (HGS Synthetic)
    

    Use

    Dictyostelium expression

    Tags

    EGFP

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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