We narrowed to 14,014 results for: TIM

1. OeNL/pcDNA3

   Plasmid

   #85202

   Purpose

   Orange color variant of bright luminescent protein

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   Orange enhanced Nano-lantern
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pG-HIV-2LTR

   Plasmid

   #104590

   Purpose

   standard for qPCR

   Depositor

   Kristine Yoder

   Article

   Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

   Insert

   2LTR circle junction of HIV-1
   

   Use

   Cloning vector

   Available Since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PT7lacO-His6-AlaRS

   Plasmid

   #224362

   Purpose

   Express AlaRS protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   AlaRS (alaS E. coli)
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEB1-mYPet

   Plasmid

   #103988

   Purpose

   Plasmid encoding mYPet

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mYPet
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (F46L, I47L, …

   Promoter

   proC

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPL5608_TEF1*-Cre_TRP1

   Plasmid

   #60931

   Purpose

   Constitutive expression vector for Cre-recombinase containing TRP1 selection marker.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Cre
   

   Tags

   Driven from a mutant TEF1 promoter with 0.16x act…

   Expression

   Yeast

   Promoter

   Mutant TEF1

   Available Since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-Ec.coaE (pESC124)

   Plasmid

   #50390

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

   Depositor

   Erick Strauss, Tadhg Begley

   Article

   Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

   Insert

   DPCK
   

   Tags

   6XHIS tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. ReNL_pRSETb

   Plasmid

   #89536

   Purpose

   Red color variant of bright luminescent protein for bacterial expression

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   ReNL
   

   Tags

   His X 6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJKR-H-cdaR

   Plasmid

   #62557

   Purpose

   Glucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.

   Depositor

   George Church

   Article

   Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

   Inserts

   beta lactamase
   sfGFP
   CdaR
   

   Expression

   Bacterial

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-link-yoEGFP-SpHis5

   Plasmid

   #44836

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized EGFP with SpHis5 selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoEGFP
   

   Expression

   Yeast

   Available Since

   May 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. [pSK600] pRK5 HA-Nprl2(R78A)

    Plasmid

    #136146

    Purpose

    mammalian expression of Nprl2

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al J Biol Chem. 2019 Feb 22;294(8):2970-297

    Insert

    Nprl2 (NPRL2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R78A

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIS.MTRIA-ATP

    Plasmid

    #184599

    Purpose

    For expression of a fluorescent sensor for ATP in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-ATP
    

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-CyRFP1

    Plasmid

    #84356

    Purpose

    Expresses CyRFP1 fluorescent protein in an empty backbone, under CAG promoter

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    CyRFP1
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. LT1_33_pTEFmut8-mCherry

    Plasmid

    #177706

    Purpose

    Construct for genome integration expressing constitutively mCherry under pTEFmut8 promoter

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    pTEFmut8-mCherry
    

    Use

    Used as donor dna in genome integration after lin…

    Expression

    Yeast

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMiniT:GFP-Gephyrin TKIT donor

    Plasmid

    #169433

    Purpose

    Donor DNA for TKIT knockin of GFP to Gephyrin in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO_NES_HaloTag9

    Plasmid

    #175537

    Purpose

    Strict cytosolic expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    NES-HaloTag9
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFA6a-link-yomCherry-SpHis5

    Plasmid

    #44841

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mCherry with SpHis5 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomCherry
    

    Expression

    Yeast

    Available Since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pH_35S/mEGFP

    Plasmid

    #135321

    Purpose

    mEGFP expression regulated by the CaMV35S promoter, co-expressed hygromycin phosphotransferase selectable marker (hpt)

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

    Insert

    mEGFP
    

    Expression

    Plant

    Promoter

    Cauliflower mosaic virus 35S

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME-Cas9

    Plasmid

    #64237

    Purpose

    expresses codon optimized Cas9 in zebrafish

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Insert

    codon optimized Cas9
    

    Use

    CRISPR

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. eMagAF-EGFP-Mito

    Plasmid

    #162243

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    eMagAF-EGFP-OMP25
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-BICD2(1-500)-FRB

    Plasmid

    #174648

    Purpose

    Rapalog-inducible coupling to dynein adaptor BICD2

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    HA-BICD2(1-500)-FRB
    

    Tags

    FRB and HA

    Expression

    Mammalian

    Mutation

    mmBICD2(aa1-500): Lys213Glu

    Promoter

    Chicken beta-actin

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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