We narrowed to 8,363 results for: construct
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Plasmid#187164Purposeoriginal vector to make all constructsDepositorInsertRight-stuffer-E1347A
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
TALED_Left-stuffer-8e
Plasmid#187162Purposeoriginal vector to make all constructsDepositorInsertstuffer-8e
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
TALED_Left-stuffer-E1347A
Plasmid#187161Purposeoriginal vector to make all constructsDepositorInsertLeft-stuffer-E1347A
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
TALED_Left-stuffer-1397N-UGI
Plasmid#187174Purposeoriginal vector to make all constructsDepositorInsertLeft-stuffer-1397N-UGI
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
TALED_Right-stuffer-1397C-UGI
Plasmid#187171Purposeoriginal vector to make all constructsDepositorInsertRight-stuffer-1397C-UGI
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-myc-FRNK
Plasmid#74481PurposeThis plasmid was constructed in order to characterize the function of Proline-rich tyrosine kinase 2 (PYK2).DepositorInsertFRNK (PTK2 Chicken)
UseTagsc-mycExpressionMammalianMutationFRNK is the C-terminal, noncatalytic domain of th…PromoterCMVAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLys-M1 backbone
Plasmid#109386PurposeEmpty plasmid of the pLys-M1 construct. This is medium copy (p15A), CmRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHFX-cas3UD
Plasmid#174385PurposePlasmids for gene knockout were constructed based on suicide plasmid pHFXDepositorInsertCas3UD
UseArchaeal gene knockout vector for haloferax medit…TagsExpressionMutationPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
plIVMD164
Plasmid#160936PurposeIn vivo mRNA display construct for GFPDepositorInsertMCP-sfGFP-HIS tag, 3'UTR MS2 Stem Loop
UseTagsHIS tag, 3'UTR MS2 Stem LoopExpressionYeastMutationPromoterMET25Available sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
LZF1723: mI12b-EGFP
Plasmid#104123PurposesynOptopatch constructDepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationPromotermI12bAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
p_3xFLAG-Rpp25
Plasmid#87194PurposeExpression of ectopic 3xFLAG-Rpp25 construct from CMV promoterDepositorInsert3xFLAG-Rpp25 (RPP25 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
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MCV-hVam6p-
Plasmid#32059DepositorInsertMCV-hVam6p-
UseTagsExpressionMutationTG to GC mutations at 3280-3281 (nt) of full cons…Promoternone from vector. Insert contains native promoter…Available sinceOct. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pYC64
Plasmid#63928PurposeRecipient replicative vector to construct FLAG tagged fusionsDepositorTypeEmpty backboneUseTagsFLAGExpressionYeastMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC62
Plasmid#63927PurposeRecipient replicative vector to construct HA tagged fusionsDepositorTypeEmpty backboneUseTagsHAExpressionYeastMutationPromoterAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Gal4pBS4-Pleum
Plasmid#60329PurposeContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBac(3xP3-gTc’v; pUb:lox-mYFP-lox-H2BmCherry)
Plasmid#119064PurposeCre/lox flip-out construct for marking cell clones in Tribolium castaneumDepositorInsertspBacL
pUb regulatory sequence
loxN
mYFP
loxN
H2B-mcherry
SV40polyA
Tc-vermilion
3xP3 TATA
pBacR
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYC25
Plasmid#63914PurposeRecipient overexpressing vector to construct YFP tagged fusionsDepositorTypeEmpty backboneUseTagsYFPExpressionYeastMutationPromoterTEF1 (Saccharomyces cerevisiae)Available sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0079
Plasmid#117646PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_ENTH/ANT {AT1G14910}(pEPOR1CB0100)and sgRNA_ENTH/ANT {AT2G01600}(pEPOR1CB0111)DepositorInsert[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYC66
Plasmid#63929PurposeRecipient replicative vector to construct MYC tagged fusionsDepositorTypeEmpty backboneUseTagsMYCExpressionYeastMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-fugu SLC2A4 promoter
Plasmid#65101PurposeLuciferase reporter construct under the fugu (Takifugu rubripes) SLC2A4 promoter.DepositorInsertSLC2A4 promoter
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAGH51
Plasmid#107255PurposeHU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.DepositorInsertHU-FRB
UseSynthetic BiologyTagsFRBExpressionMutationPromoternative HU promoterAvailable sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-myc-Med20 pBacPAK8
Plasmid#15370DepositorInsertMed20
UseBaculovirus constructionTagsHis and mycExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYC16
Plasmid#63913PurposeRecipient overexpressing vector to construct CFP tagged fusionsDepositorTypeEmpty backboneUseTagsCFPExpressionYeastMutationPromoterTEF1 (Saccharomyces cerevisiae)Available sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Px-mCerulean-pA
Plasmid#202048PurposeEncodes mCerulean, which can be expressed with the activation of a synthetic promoter by Cas enzymes. A cloning site is provided to construct the synthetic Cas-targeted promoter upstream of mCerulean.DepositorInsertmCerulean
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Px-mCherry-pA
Plasmid#202049PurposeEncodes mCherry, which can be expressed with the activation of a synthetic promoter by Cas enzymes. A cloning site is provided to construct the synthetic Cas-targeted promoter upstream of mCherry.DepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Px-PhiYFP-pA
Plasmid#202045PurposeEncodes PhiYFP, which can be expressed with the activation of a synthetic promoter by Cas enzymes. A cloning site is provided to construct the synthetic Cas-targeted promoter upstream of PhiYFP.DepositorInsertPhiYFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-21_MBP-VidN
Plasmid#241013PurposeVidN construct that can be bacterially expressed, used to demonstrate intein splicing reaction.DepositorInsertMBP-VidN
UseTagsMBP-tagExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJM220-PhoA-mNeongreen-J23101-mScarletI
Plasmid#240201PurposeMiniTn7 donor plasmid for constructing a ratiometric reporter for P limitation in Pseudomonas synxantha. A strong Anderson promoter (J23101) drives expression of mScarlet-IDepositorInsertsmNeongreen
mScarlet-I
UseTagsExpressionMutationPromoterJ23101 and PphoAAvailable sinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJM220-PphoA-mNeongreen-J23116-mScarletI
Plasmid#240200PurposeMiniTn7 donor plasmid for constructing a ratiometric reporter for P limitation in Pseudomonas synxantha. A weak Anderson promoter (J23116) drives expression of mScarlet-IDepositorInsertsmNeongreen
mScarlet-I
UseTagsExpressionMutationPromoterJ23116 and PphoAAvailable sinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSWITCHER-TF1-dsRED
Plasmid#213043PurposeSWITCHER ready LoxP-EGFP reporter plasmid for cloning of a customized CRISPR-inducible Cre-constructDepositorInsertSWITCHER-TF1-dsRED
UseCRISPRTagsExpressionMammalianMutationPromotercBHAvailable sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSplit-SWITCHER1-TF1-dsRED
Plasmid#213044PurposeA CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassetteDepositorInsertSplit-SWITCHER1-TF1-dsRED
UseCRISPRTagsExpressionMammalianMutationPromotercBHAvailable sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSplit-SWITCHER2-TF1-dsRED
Plasmid#213045PurposeA CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassetteDepositorInsertSplit-SWITCHER1-TF1-dsRED
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6-LbCpf1-crF1
Plasmid#213046PurposeGuide RNA expression vector with the synthetic crRNA crF1 targeting the SWITCHER construct with the target site TF1DepositorInsertcrF1
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6-LbCpf1-crR2
Plasmid#213047PurposeGuide RNA expression vector with the synthetic crRNA crR2 targeting the SWITCHER construct with the target site TR2DepositorInsertcrR2
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLoxP-EGFP-crRNA-entry
Plasmid#213048PurposeSWITCHER ready LoxP-EGFP reporter plasmid for cloning of a customized CRISPR-inducible Cre-constructDepositorInsertLoxP-EGFP-MALAT1-triplex
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
POC1431
Plasmid#221578PurposeAssembled plasmid containing four inserts (from POC1426, POC1427, POC1428 and POC1429) assembled using the site-selective methylation protection approachDepositorInsertAssembled construct flanked by methylation-protectable BsaI sites and containing four always-methylable internal BsaI sites
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-dCas9-VPR-pgk-hph
Plasmid#233066PurposePiggybac CRISPRa constructDepositorInsertdCas9-VPR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_mutCKAR3
Plasmid#238414PurposeAAV construct with CKAR3 mutant expression under a human synapsin promotor and WPRE3 translation enhancer.DepositorInsertphosphorylation deficient mutant of CKAR3 (T to A)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_Zdk3-VPR_P2A_dCas9-AsLOVx3-NLSx3
Plasmid#231568PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertAll-in-one LOOMINA construct
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-sfGFP(1-157)
Plasmid#220743PurposeGAL4-sfGFP(1-157) construct expression in mammlian cells.DepositorInsertGAL4-sfGFP(1-157)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-sfGFP(158-238)
Plasmid#220744PurposeGAL4-sfGFP(158-238) construct expression in mammlian cells.DepositorInsertGAL4-sfGFP(158-238)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-NIR-FbLAG30
Plasmid#220746PurposeGAL4-NIR-FbLAG30 construct expression in mammlian cells.DepositorInsertGAL4-NIR-FbLAG30
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pETSUMOSyt1C2AB(80-421)
Plasmid#209005PurposeRecombinant protein expression of truncated syt1 constructDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 80-421 onlyPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(96-421)
Plasmid#209006PurposeRecombinant protein expression of truncated syt1 constructDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 96-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(143-421)
Plasmid#209007PurposeRecombinant protein expression of truncated syt1 constructDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 143-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2144
Plasmid#190344PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::GFPint-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB3410
Plasmid#190410PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proAA::GFPint-ADHter)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proAA::GFPint-ADHter
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only