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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,346 results for: 1186

Showing: 2201 - 2220 of 2346 results

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  2. pSHUT4-eYFP

    Plasmid
    #133094
    Purpose
    Agrobacterium T-DNA overexpression cassette comprising constitutive TEF-1alpha fused to reporter eYFP. Integrates near beta-tubulin gene in F. solani genome. Replace eYFP w/ XhoI BamHI
    Depositor
    Jens Laurids Sørensen
    Article
    Nielsen et al Fungal Biol Biotechnol. 2019 Dec 11;6:25
    Insert
    enhanced yellow flourescent protein
    Use
    Fungal expression
    Tags
    Expression
    Mutation
    Promoter
    TEF-1α
    Available Since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133094-plasmid-map-sequence-id-266874

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  7. pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP

    Plasmid
    #176027
    Purpose
    construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry
    Depositor
    Irmela Jeremias
    Article
    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1
    Inserts
    rtTA3
    Luc2
    mtagBFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    176027-plasmid-map-sequence-id-351264

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  8. pAAV-CaMKII-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

    Plasmid
    #165493
    Purpose
    Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity
    Depositor
    Thomas Oertner
    Article
    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10
    Insert
    2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
    Use
    AAV
    Tags
    darkVenus
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKII
    Available Since
    Oct. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165493-plasmid-map-sequence-id-330312

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  10. pLKO.1-shGBP1.1.mKO2

    Plasmid
    #85209
    Purpose
    TRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.
    Depositor
    Sandra Martha Gomes Dias
    Article
    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1
    Insert
    GBP1 (guanylate binding protein 1) (GBP1 Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RNA polymerase III promoter for human U6 snRNA fo…
    Available Since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    85209-plasmid-map-sequence-id-169780

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  11. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid
    #92102
    Purpose
    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    Cbh
    Available Since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92102-plasmid-map-sequence-id-197631

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  12. tRNA-gRNA position [D1_n-1] (GB1208)

    Plasmid
    #75409
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_n-1]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter (2-part multiplexing)
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA position [D1_n-1]
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75409-plasmid-map-sequence-id-211946

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  13. pDONR P4-P1R-EGFP

    Plasmid
    #48348
    Purpose
    Contributes the coding sequence for EGFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Depositor
    Anna Planas, Tomas Santalucia
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    EGFP
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Contains a Kozak sequence. Does not contain a sto…
    Promoter
    none
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48348-plasmid-map-sequence-id-70559

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  18. pAAV-Syn-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

    Plasmid
    #165492
    Purpose
    Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity
    Depositor
    Thomas Oertner
    Article
    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10
    Insert
    2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
    Use
    AAV
    Tags
    darkVenus
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn1
    Available Since
    Oct. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165492-plasmid-map-sequence-id-330311

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  20. tRNA-gRNA position [D1_2] (GB1205)

    Plasmid
    #75406
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_2]) of a polycistronic tRNA-gRNA regulated by the dicot U6-26 or U6-1 promoter (3-part multiplexing)
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75406-plasmid-map-sequence-id-146004

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