We narrowed to 2,382 results for: T2A

1. pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

   Plasmid

   #64217

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   hU6 promoter; BbsI sites for sgRNA
   H1 promoter; BamHI site for shRNA
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh, H1, and U6

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFH2.107_PB-EPN24-MCP_p6-T2A-eGFP-PGK-Blast

   Plasmid

   #205552

   Purpose

   Express EPN24-MCP-p6-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN24-MCP-p6
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4

   Plasmid

   #78349

   Purpose

   Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

   Depositor

   Michael Elowitz

   Article

   Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

   Insert

   H2B-mCherry-T2A-rTetR-HDAC4 (HDAC4 Human, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   rTetR

   Expression

   Mammalian

   Mutation

   Promoter

   pEF alpha

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR

   Plasmid

   #199197

   Purpose

   Insert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digest

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[1-20]-N-int-BlastR
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB713B-pCMV-MITD-NeoMini-T2A-tCD19-WPRE

   Plasmid

   #174610

   Purpose

   PiggyBack transposon expressing a fusion of a tandem minigene antigens in KRAS, BRAF and CMV fused to an endosomal targeting sequence and translationally linked to human truncated CD19

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   human CD19, antigen minigene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-Flex-DN-AMPK-K45R-T2A-mCherry

   Plasmid

   #83491

   Purpose

   cre-dependent DN-AMPK under EF1a promoter

   Depositor

   Dong Kong

   Article

   Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

   Insert

   DN-AMPK-2A-mCherry
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   K45R

   Promoter

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry

   Plasmid

   #124431

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafish

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq) T2A mCherry
   

   Use

   Zebrafish (if expression level is too high in zeb…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-EF1S-Nluc-P2A-copGFP-T2A-Puro

   Plasmid

   #73032

   Purpose

   Express fluorescent and luminescence dual reporter

   Depositor

   Kazuhiro Oka

   Insert

   Nluc-P2A-copGFP-T2A-Puro
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Truncated EF1

   Available since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

   Plasmid

   #109428

   Purpose

   A lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   mCherry T2A GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Inserted 43 base pairs into mCherry to create rep…

   Promoter

   CMV

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDropIn-T2A-split-intein-Gal4[21-881]-C-int

    Plasmid

    #199196

    Purpose

    Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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